Practical aspects of DNA-based forensic studies in dentistry

Muruganandhan, Jayanandan; Sivakumar, G

doi:10.4103/0975-1475.85295

Cited by 24 publications

(17 citation statements)

References 16 publications

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“…However, research has revealed that varying qualities and quantities of DNA are extracted from different individuals from the same site, even under similar conditions (Muruganandhan and Sivakumar, 2011). Therefore, the DNA recovered from the evidence may have (by chance) been of sufficient quality and quantity to facilitate STR typing in this case.…”

Section: Discussionmentioning

confidence: 99%

Relevance of sampling and DNA extraction techniques for the analysis of salivary evidence from bite marks: a case report

Ml¹,

Hernández-Cortés²,

Jaramillo‐Rangel³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Bite mark evidence has been repeatedly found in criminal cases. Physical comparison of a bite mark to the teeth of available suspects may not always be possible. Experimental studies have shown that the analysis of DNA present in the saliva recovered from bite marks might help in the identification of individuals. However, the application of this approach to an actual criminal case has been reported only once before in forensic literature. Therefore, there is very limited scientific and technical information available on this subject. The current study focuses on a woman found dead in her home; the autopsy ruled the death to be a result of manual strangulation. A bite mark was found on each breast. The single swab technique was used to collect evidence from these bite marks, and an organic extraction method was employed for DNA isolation. Short tandem repeat (STR) sequence typing was performed using a commercially available kit, and the result was compared to the STR profile of a suspect. A full singlesource STR profile was obtained from both bite marks, which matched the STR profile of the suspect. To the best of our knowledge, this is the second report on the analysis of DNA isolated from bite marks on the victim used to identify the crime perpetrator. Our results indicated that, contrary to most theoretical indications, a single swab technique for evidence collection and an organic method for DNA isolation could be very useful in solving this class of criminal cases.

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Section: Discussionmentioning

confidence: 99%

Relevance of sampling and DNA extraction techniques for the analysis of salivary evidence from bite marks: a case report

Ml¹,

Hernández-Cortés²,

Jaramillo‐Rangel³

et al. 2015

Genet. Mol. Res.

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show abstract

“…Saliva is deposited in the skin at the time of biting and should be collected - preferably using the double cotton swab technique ( 36 ). Dry saliva is hard to detect, and the amylase test is needed to identify its presence ( 37 ).…”

Section: Human Bites As a Methods For Identifying An Aggressormentioning

confidence: 99%

“…The main DNA source is blood, though in some situations this type of sample is not available for analysis. In teeth, DNA is found in the pulp tissue, dentin, cement, periodontal ligament and alveolar bone ( 37 ). Due to the resistance of the hard tissues of the teeth to environmental actions such as incineration, immersion, trauma or decomposition, pulp tissue is an excellent source of DNA ( 5 ).…”

Section: The Role Of Dna In Dental Identificationmentioning

confidence: 99%

Forensic dentistry in human identification: A review of the literature

Ata-Ali

2014

J Clin Exp Dent

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An update is provided of the literature on the role of odontology in human identification, based on a PubMed-Medline search of the last 5 years and using the terms: “forensic dentistry” (n = 464 articles), “forensic odontology” (n = 141 articles) and “forensic dentistry identification” (n = 169 articles). Apart from these initial 774 articles, others considered to be important and which were generated by a manual search and cited as references in review articles were also included. Forensic dentistry requires interdisciplinary knowledge, since the data obtained from the oral cavity can contribute to identify an individual or provide information needed in a legal process. Furthermore, the data obtained from the oral cavity can narrow the search range of an individual and play a key role in the victim identification process following mass disasters or catastrophes. This literature search covering the last 5 years describes the novelties referred to buccodental studies in comparative identification, buccodental evaluation in reconstructive identification, human bites as a method for identifying the aggressor, and the role of DNA in dental identification. The oral cavity is a rich and noninvasive source of DNA, and can be used to solve problems of a social, economic or legal nature. Key words:Forensic identification, DNA, forensic dentistry, rugoscopy, cheiloscopy, saliva.

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“…The tooth may not contain pulp at all due to previous endodontic therapy or it 5 may be infected, contaminating the DNA samples . Dentine has also been found to be a potential source of mitochondrial DNA 5,6 when the quantity and quality of available DNA is limited .…”

Section: Tooth Sectioningmentioning

confidence: 99%

Quantitative evaluation of DNA from the tooth pulp exposed to varying temperatures

Ibrahim

Bhat

Shenoy³

et al. 2016

Journal of Health and Allied Sciences NU

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Context: During natural calamities like fire accidents, many times the human body gets charred beyond recognition. Tooth, being the hardest structure in the human body protects the pulp within from such accidents. Forensic identification through the pulpal DNA could be avery useful tool in such situations. Aim: This study was designed to evaluate the quantity of DNA obtained from the pulp when exposed to varying temperatures. Methods and Material: Extracted teeth were subjected to the following temperatures:-80°C,37°C,100°C, 200°C, 300°C, 500°C and 1000°C. Pulp from these teeth were retrieved by horizontally sectioning the teeth using a fine needle diamond point in a high speed air rotor, followed by extraction of DNA with the HipuraA™ Forensic Genomic DNA purification spink it. The samples were quantified using personal computer(PC) based double beam spectro photo meter. Kruskal Wallis test and Mann WhitneyU test were used for statistical analysis. Results: Statistically significant difference (p<0.005) was seen in the quantity of DNA obtained from pulp subjected to higher temperatures as compared to oral temperature. However, there was no significant difference (p>0.05) in the quantity of DNA obtained from teeth subjected to-80°C Conclusions: Increase in temperature decreases the amount of DNA from the tooth pulp where as a decrease in temperature does not cause any change in the quantity of DNA.

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Practical aspects of DNA-based forensic studies in dentistry

Cited by 24 publications

References 16 publications

Relevance of sampling and DNA extraction techniques for the analysis of salivary evidence from bite marks: a case report

Relevance of sampling and DNA extraction techniques for the analysis of salivary evidence from bite marks: a case report

Forensic dentistry in human identification: A review of the literature

Quantitative evaluation of DNA from the tooth pulp exposed to varying temperatures

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