Practical considerations in the chiral separation of dns-amino acids by reversed-phase liquid chromatography using metal chelated additives

Tapuhi, Yitzhak; Miller, Neil T.; Karger, Barry L.

doi:10.1016/s0021-9673(00)82660-6

Cited by 132 publications

(13 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fluorescence detection was by excitation at 339 nm and emission at 513 nm. The sensitivity of detection and precision of assay of these aminoacids where similar to those previously reported for other Dns-aminoacids ( 19). Hydrolysis experiment.…”

Section: Isolation Of Peripheral Blood Mononuclear Cells (Pbmc)supporting

confidence: 84%

Generation of Human Lak Cells in Tissue Culture Bags Using Recombinant Il-2 and Serum Free Medium Effects of pretreatment with phenylalanine-methylester

Wersäll

Masucci²,

Pihlstedt³

et al. 1990

Acta Oncologica

View full text Add to dashboard Cite

Section: Isolation Of Peripheral Blood Mononuclear Cells (Pbmc)supporting

confidence: 84%

Generation of Human Lak Cells in Tissue Culture Bags Using Recombinant Il-2 and Serum Free Medium Effects of pretreatment with phenylalanine-methylester

Wersäll

Masucci²,

Pihlstedt³

et al. 1990

Acta Oncologica

View full text Add to dashboard Cite

“…Amino acids were recovered quantitatively in the eluate from the resin washed with five bed volumes of ammonia (2 N) (18). The amino acid fraction was dried by rotary film evaporation and excess ammonia was removed from the residue while it was stored in the presence of concentrated H2SO4 at reduced pressure for 8 h. The residue was dissolved in water to give 0.05 to 0.5 mM amino acids and these were converted to the corresponding 5-dimethylaminonaphthalene-l-sulfonamino acids (DNS-amino acids) using DNS-Cl as previously described (24) except that the volumes were reduced to one-tenth and the reaction was not terminated with an amine. Instead,20 ,u vulgaris exposed to phaseolotoxin.…”

Section: Methodsmentioning

confidence: 99%

Association between Symptom Development and Inhibition of Ornithine Carbamoyltransferase in Bean Leaves Treated with Phaseolotoxin

Turner¹,

Mitchell²

1985

Plant Physiol.

View full text Add to dashboard Cite

The chlorosis symptom that characterizes the halo blight disease of Phaseolus vulgaris L. is caused by phaseolotoxin produced by the plant pathogenic bacterium Pseudomonas syringae pv phaseolicola. Phaseolotoxin is hydrolyzed by plant peptidases to N(N'-sulpho-diaminophosphinyl)-L-ornithine which also causes chlorosis and is reported to be an irreversible inhibitor of ornithine carbamoyltransferase (OCIase). We have examined the hypothesis that inhibition of OClase is the primary action of phaseolotoxin that leads to chlorosis.Chlorotic spots appeared on the primary leaves of P. vulgars seedlings during the 2 days following leaf prick application of a minimum of 30 picomole phaseolotoxin. OCTase in extracts of the lesions was reduced to 20%, or less, of the activity in controls. Four hours after the application of phaseolotoxin the concentration of free ornithine increased more than 2-fold. Other amino acids, especially glutamine and asparagine-but not arginine-increased later. Chlorophyll remained at a constant level in the phaseolotoxin-treated tissue and the appearance of chlorosis was due to the increase in chlorophyll in the surrounding unaffected tissue.Clear halo symptoms developed only on primary leaves of the youngest seedlings (treated 6-7 days after germination). Lesions did not develop on primary leaves of seedlings more than 14 days old, in which the chlorophyll concentration had reached a maximum. OCIase also was inhibited in the symptomless tissue from older leaves treated with phaseolotoxin, but there was no accumulation of amino acids, including ornithine. A single appliction of 200 nanomoles arginine resulted in the complete regreening of the chlorosis caused by phaseolotoxin. Soluble protein was lower in the chlorotic tissue than in the controls, but increased to greater than the control value following the appliction of arginine.These results suggest that phaseolotoxin-induced chlorosis results from reduced chlorophyll synthesis that is associated with arginine-starvation in the tissue where OCTase is inhibited.

show abstract

“…Dansylation was performed using the method described by Tapuhi et al [19] with minor modifications. The reaction temperature and time were optimized in the preliminary study.…”

Section: Dansylationmentioning

confidence: 99%

Determination of muscimol and ibotenic acid in Amanita mushrooms by high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry

Tsujikawa

Kuwayama

Miyaguchi

et al. 2007

Journal of Chromatography B

View full text Add to dashboard Cite

Practical considerations in the chiral separation of dns-amino acids by reversed-phase liquid chromatography using metal chelated additives

Cited by 132 publications

References 30 publications

Generation of Human Lak Cells in Tissue Culture Bags Using Recombinant Il-2 and Serum Free Medium Effects of pretreatment with phenylalanine-methylester

Generation of Human Lak Cells in Tissue Culture Bags Using Recombinant Il-2 and Serum Free Medium Effects of pretreatment with phenylalanine-methylester

Association between Symptom Development and Inhibition of Ornithine Carbamoyltransferase in Bean Leaves Treated with Phaseolotoxin

Determination of muscimol and ibotenic acid in Amanita mushrooms by high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry

Contact Info

Product

Resources

About