Practical experience with commercial embryo transfer in pigs

Cameron, R. D. A.; Durack, M.; Fogarty, R; Putra, D.K.H.; McVeigh, J. F.

doi:10.1111/j.1751-0813.1989.tb09714.x

Cited by 32 publications

(12 citation statements)

References 5 publications

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“…Litter size at birth was 6.8, while 15-21 embryos were transferred. There are no indications that the transfer conditions in this study are any different from established collection conditions (embryo collection by slaughter: Schlieper and Holtz 1986) or surgical-transfer conditions (medium and temperature: Schlieper and Holtz 1986;Cameron et al 1989;Niemann et al 1989). The only essential difference to the established procedures was the transcervical deposition of the embryos in the uterus near the cervical junction.…”

Section: Discussionmentioning

confidence: 93%

Farrowing Rate and Litter Size after Transcervical Embryo Transfer in Sows

Hazeleger¹,

Kemp²

1994

Reprod Domestic Animals

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Section: Discussionmentioning

confidence: 93%

Farrowing Rate and Litter Size after Transcervical Embryo Transfer in Sows

Hazeleger¹,

Kemp²

1994

Reprod Domestic Animals

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“…The cervical folds and the length and coiled nature of the pig uterine horns have been the principal obstacles for the development of an effective procedure for non-surgical ET over the past decades [1]. In the 1990s, several non-surgical procedures to deposit morulae and/or blastocysts into the cervix or uterine body were developed [2], [3], but the farrowing rates (<40%) and litter sizes (5–7.5 piglets born) were lower compared with those previously reported using surgical ET (farrowing rates: 80%; litter sizes: 8 piglets born) [4]. Because the middle and last third of the uterine horn are more physiologically appropriate locations for these embryos than the cervix or the uterine body, we developed a new and unique procedure for non-surgical deep uterine (NsDU) transfer of porcine embryos with acceptable and promising reproductive performance of the recipients (71.4% farrowing rate and 6.9 piglets born) [5].…”

Section: Introductionmentioning

confidence: 99%

Successful Non-Surgical Deep Uterine Transfer of Porcine Morulae after 24 Hour Culture in a Chemically Defined Medium

et al. 2014

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Excellent fertility and prolificacy have been reported after non-surgical deep uterine transfers of fresh in vivo-derived porcine embryos. Unfortunately, when this technology is used with vitrified embryos, the reproductive performance of recipients is low. For this reason and because the embryos must be stored until they are transferred to the recipient farms, we evaluated the potential application of non-surgical deep uterine transfers with in vivo-derived morulae cultured for 24 h in liquid stage. In Experiment 1, two temperatures (25°C and 37°C) and two media (one fully defined and one semi-defined) were assessed. Morulae cultured in culture medium supplemented with bovine serum albumin and fetal calf serum at 38.5°C in 5% CO2 in air were used as controls. Irrespective of medium, the embryo viability after 24 h of culture was negatively affected (P<0.05) at 25°C but not at 37°C compared with the controls. Embryo development was delayed in all experimental groups compared with the control group (P<0.001). Most of the embryos (95.7%) cultured at 37°C achieved the full or expanded blastocyst stage, and unlike the controls, none of them hatched at the end of culture. In Experiment 2, 785 morulae were cultured in the defined medium at 37°C for 24 h, and the resulting blastocysts were transferred to the recipients (n = 24). Uncultured embryos collected at the blastocyst stage (n = 750) were directly transferred to the recipients and used as controls (n = 25). No differences in farrowing rates (91.7% and 92.0%) or litter sizes (9.0±0.6 and 9.4±0.8) were observed between the groups. This study demonstrated, for the first time, that high reproductive performance can be achieved after non-surgical deep uterine transfers with short-term cultured morulae in a defined medium, which opens new possibilities for the sanitary, safe national and international trade of porcine embryos and the commercial use of embryo transfer in pigs.

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“…6), which indicated that more vitrified embryos are necessary for nonsurgical ETs than for surgical ETs. This is not a surprising finding because successful nonsurgical ET requires almost twice the number of fresh embryos recommended for surgical transfers [4,51,53]. A reasonable explanation for the difference may be the site of deposition of the embryo within the uterus, which is related to the ET procedure used.…”

Section: Recipients (N) Embryos Transferred (N) Embryo Transfer Methomentioning

confidence: 80%

“…Overall, these studies have reported pregnancy rates of 60% to 90% with 8 to 10 fetuses per pregnant recipient at 25 to 40 days of pregnancy [13,48,50] or farrowing rates and litter sizes of 60% to 80% and 7 to 8 piglets born, respectively [51,52]. These results are higher than those reported after nonsurgical ET into the uterine body (farrowing rates of 33%-40%; litter sizes of 6.7-7.4 piglets) [46,47] but similar to those achieved in the first attempt of the NsDU-ET technique (71.4% farrowing rate and 6.9 piglets/litter) [4].…”

Section: Fresh and Short-term-stored Embryosmentioning

confidence: 94%