2020
DOI: 10.1002/wrna.1633
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Practical guide for circular RNA analysis: Steps, tips, and resources

Abstract: Recent technological advances in RNA sequencing and analysis have allowed an increasingly thorough investigation of a previously unexplored class of transcripts, circular (circ)RNAs. Accumulating evidence suggests that circRNAs have unique functions which often rely on their association with microRNAs and RNA-binding proteins. Through these interactions, circRNAs have been implicated in major cellular processes and hence in the pathophysiology of a range of diseases. Here, we provide guidelines to consider whe… Show more

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Cited by 17 publications
(11 citation statements)
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“…Moreover, the possible functions of these circRNAs could be validated by visualizing their distribution and cellular localization by circFISH [ 66 ], or by verifying in which compartments are the circRNAs localized by circRNA expression profiling among the subcellular fractions [ 67 ]. In addition, the circRNA–miRNA interactions could be further examined by tagging a novel PRMT1 circRNA for affinity pulldown assays using a biotinylated antisense oligonucleotide that targets the back-splice junction and can be pulled down specifically with streptavidin-coated beads [ 68 ]. However, there lies a limitation of our study.…”
Section: Discussionmentioning
confidence: 99%
“…Moreover, the possible functions of these circRNAs could be validated by visualizing their distribution and cellular localization by circFISH [ 66 ], or by verifying in which compartments are the circRNAs localized by circRNA expression profiling among the subcellular fractions [ 67 ]. In addition, the circRNA–miRNA interactions could be further examined by tagging a novel PRMT1 circRNA for affinity pulldown assays using a biotinylated antisense oligonucleotide that targets the back-splice junction and can be pulled down specifically with streptavidin-coated beads [ 68 ]. However, there lies a limitation of our study.…”
Section: Discussionmentioning
confidence: 99%
“…CircRNA is a type of non-coding RNA widely distributed in eukaryotes. CircRNA is highly stable as it is resistant to exonuclease- or ribonuclease-mediated degradation ( 13 ). It has been reported that circRNA is a key regulator of tumor gene expression and serves key roles in the signaling networks in oral squamous cell carcinoma, including the Wnt/β-catenin and PI3K/AKT pathways ( 14 ).…”
Section: Discussionmentioning
confidence: 99%
“…Circular RNAs (circRNAs) are a novel class of discovered ncRNAs with structural stability, formed by reverse splicing of the 3′-end and 5′-end of a chain-like pre-mRNA [ 43 , 44 ]. In general, circRNAs are formed via exon or intron cyclization, has no 3′-end or 5′-end of linear RNA, their structure is very stable and cannot be hydrolyzed by ordinary RNase; [ 45 47 ]. Circular RNA homeodomain-interacting protein kinase three (circHIPK3) is produced from exon two of HIPK3 and have been studied in many recent studies.…”
Section: Introductionmentioning
confidence: 99%