Practical guide to medical student assessment

Complete genome sequence and construction of infectious full-length cDNA clones of tobacco ringspot Nepovirus, a viral pathogen causing bud blight in soybean

“…Purified particles examined by transmission electron microscopy (Philips CM20, Eindhoven, Netherlands) were observed to contain the three different components referred to as component B, M, and T (Fig. 1c) [9]. For Western blot analysis, total protein was isolated from both infiltrated and upper uninfiltrated leaves of N. benthamiana plants displaying viral symptoms at 9 days after agroinfiltration with the extraction buffer (125 mM Tris-Cl, pH 8.8, 1 % SDS, 10 % Glycerol, 50 mM Na 2-S 2 O 5 and 1 mM PMSF).…”