Practical marker-assisted selection using two SCAR markers for fruit astringency type in crosses of ‘Taiten’×PCNA cultivars in persimmon breeding

Mitani, Nobuhito; Kono, Atsushi; Yamada, Masahiko; Sato, Akihiko; Kobayashi, Shozo; Ban, Yusuke; Ueno, Takatoshi; Shiraishi, Masayuki; Kanzaki, Sho; Tsujimoto, Tomoyuki; Yonemori, Keizo

doi:10.1016/j.scienta.2014.03.001

Cited by 14 publications

(9 citation statements)

References 23 publications

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“…These SCAR markers have been applied to offspring from two backcross families using 'Taigetsu' (J-PCNA × 'Kurokuma'; Yamada et al 2012b) and 'Taiten' (J-PCNA × 'Kurokuma'; Yamada et al 2012c). The effectiveness of these markers has been confirmed in practical persimmon breeding (Mitani et al 2014a(Mitani et al , 2014b.…”

Section: Development Of Molecular Markers For Discriminating J-pcna Amentioning

confidence: 93%

Persimmon breeding in Japan for pollination-constant non-astringent (PCNA) type with marker-assisted selection

Sato

Yamada

2016

Breed. Sci.

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Oriental persimmon (Diospyros kaki) originated in Eastern Asia, and many indigenous cultivars have been developed in China, Japan, and Korea. These cultivars are classified into four groups based on their natural astringency loss on the tree and seed formation: pollination-constant non-astringent (PCNA), pollinationvariant non-astringent (PVNA), pollination-constant astringent (PCA), and pollination-variant astringent (PVA). PCNA is the most desirable type because the fruit can be eaten without any postharvest treatment; therefore, one of the goals of our persimmon breeding programs is to release superior PCNA cultivars. The PCNA genotype is recessive to the other three non-PCNA genotypes, and PCNA-type F 1 offspring are obtained exclusively from crosses among PCNA genotypes. Moreover, the number of superior PCNA crossparents have been limited. In the late 1980s, inbreeding depression became obvious, especially in terms of fruit size, tree vigor, and productivity. To mitigate the inbreeding, a backcross program using PCNA [(non-PCNA × PCNA) × PCNA] was started in 1990. This process, however, was inefficient because only 15% of the offspring were PCNA, and all offspring had to be grown to the fruiting stage. Therefore, molecular markers linked to the PCNA locus were developed for discriminating PCNA offspring. A molecular marker linked to Chinese PCNA has also been developed.

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Section: Development Of Molecular Markers For Discriminating J-pcna Amentioning

confidence: 93%

Persimmon breeding in Japan for pollination-constant non-astringent (PCNA) type with marker-assisted selection

Sato

Yamada

2016

Breed. Sci.

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“…To select the minimum primer combination for selecting PCNA at seedling stage, E4/E9r and AST-F/PCNA-F/5R3R were considered as appropriate markers for use in PCNA MAS. But Mitani et al (2014) assessed use of SCAR markers E4/A2r and 7H9F/AST-R for selecting astringency type in segregating populations derived from crossing 'Taiten' with PCNA cultivars. However, no E4/A2r PCR products specific for the ast allele were observed despite using identical PCR conditions as previously described (Mitani et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…But Mitani et al (2014) assessed use of SCAR markers E4/A2r and 7H9F/AST-R for selecting astringency type in segregating populations derived from crossing 'Taiten' with PCNA cultivars. However, no E4/A2r PCR products specific for the ast allele were observed despite using identical PCR conditions as previously described (Mitani et al 2014). In addition, it is noteworthy that these markers can produce PCR products from plants in a segregating population derived from crossing with a Korean cultivar 'Cheongdo-Bansi' (Table 1), even though the markers originated from Japanese cultivars.…”

Section: Discussionmentioning

confidence: 99%

Efficiency of Sequence Characterized Amplified Region Markers for Selecting Non-Astringent Persimmon (Diospyros kaki Thunb.)

Park¹,

Jae²,

Shon³

et al. 2016

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Persimmon (Diospyros kaki Thunb.) is classified into four types based on the fruit traits, astringency and flesh color. Of the four types, the pollination-constant non-astringent (PCNA) fruit is typically most desirable for consumption. In the present study, we used five sequence characterized amplified region (SCAR) markers associated with astringency in persimmon fruit, namely E4/E9r, E4/A2r, 7H9F/AST-R, AST-F/AST-R, and AST-F/PCNA-F/5R3R, to improve the efficiency of PCNA-type persimmon breeding via marker-assisted selection (MAS). A total of 84 cultivars of the four types and their segregating F1 progeny were used to evaluate the association of SCAR markers with the fruit astringency phenotype. Polymerase chain reaction evaluation of each SCAR marker showed that E4/E9r combined with AST-F/PCNA-F/5R3R was appropriate for selecting the ast allele responsible for PCNA-type fruit, as the phenotype-genotype match percentages of these two markers were 94% and 99%, respectively. This MAS was verified by the successful use of AST-F/PCNA-F/5R3R to select 107 PCNA-type individuals from 609 F1 hybrid progeny derived from various crosses.

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“…The D. kaki population, named the B515 population, was made from a cross between 'Taiten' (non-PCNA; A 2 A 3 aaaa) and 'Kanshu' (PCNA; aaaaaa) cultivars, with the resulting plants grown in the orchard of the Grape and Persimmon Research Division, NARO Institute of Fruit Tree and Tea Science, Japan. The AST genotype of the parents has been reported by Mitani et al (2014). A 2 and A 3 are dominant AST alleles while a is a recessive allele.…”

Section: Plant Materials and Phenotypingmentioning

confidence: 99%

“…Two functional AST alleles, A 2 and A 3 , are present in the D. kaki B515 population (Mitani et al, 2014). Molecular markers that could separately detect the A 2 and A 3 haplotypes of B515 were developed to improve the genetic characterization of the AST locus in this study.…”

Section: Linkage Analysismentioning

confidence: 99%

Comparative Mapping of the ASTRINGENCY Locus Controlling Fruit Astringency in Hexaploid Persimmon (Diospyros kaki Thunb.) with the Diploid D. lotus Reference Genome

et al. 2018

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Persimmon (Diospyros kaki) is a tree crop species that originated in East Asia, consists mainly of hexaploid individuals (2n = 6x = 90) with some nonaploid individuals. One of the unique characteristics of persimmon is the continuous accumulation of proanthocyanidins (PAs) in its fruit until the middle of fruit development, resulting in a strong astringent taste even at commercial fruit maturity. Among persimmon cultivars, pollination-constant and non-astringent (PCNA) types cease PA accumulation in early fruit development and become non-astringent at commercial maturity. PCNA is an allelic trait to non-PCNA and is controlled by a single locus called the ASTRINGENCY (AST) locus. Previous segregation analyses indicated that the AST locus shows hexasomic inheritance; a recessive allele, ast, at this locus confers PCNA. Here, we report a shuttle mapping approach to delimit the AST locus region in the hexaploid persimmon genome by using D. lotus, a diploid relative of D. kaki, as a reference. A D. lotus F 1 population of 333 individuals and 296 D. kaki siblings segregating for the PCNA trait were used to map the AST region using haplotype-specific markers covering the AST region. This indicated that the AST locus is syntenic to an approximately 915-kb region of the D. lotus genome. In this 915-kb region, we found several candidates for AST that were revealed from the fruit transcriptome of a population segregating for the PCNA trait. These results could provide important clues for the isolation of AST in hexaploid persimmon.Key Words: candidate gene, chromosome walking, polyploidy, proanthocyanidins. IntroductionProanthocyanidins (PAs) are a class of final products of the flavonoid biosynthetic pathway that generally have protective roles against biotic and abiotic stresses. PAs are potentially attractive compounds in terms of human health because of their scavenging capacity for free radicals; PAs are also known to confer astringency or bitterness to fruit and vegetables (Aron and Kennedy, 2008;Bagchi et al., 2014;Dixon et al., 2005). The commercial and biological significance of PA biosynthesis has resulted in extensive studies being conducted on the PA biosynthetic pathway and its transcriptional regulators, especially in the model plant Arabidopsis thaliana (Lepiniec et al., 2006;Saito et al., 2013;Xu et al., 2015). Persimmon (Diospyros kaki Thunb.), an economically important fruit crop cultivated in temperate and Mediterranean climates, shows a distinctive characteristic of PA accumulation in its fruit. In persimmon fruit, the mean degree of polymerization of PA is high (approximately 10-40), and unlike other important fruit crops, persimmon fruit accumulates a vast amount of PA until the middle of fruit development, resulting in a This article is an Advance Online Publication of the authors' corrected proof. Note that minor changes may be made before final version publication. strong astringent taste even at commercial maturity (Akagi et al., 2009a(Akagi et al., , 2011. Depending on the genotype, persimmon fru...

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Practical marker-assisted selection using two SCAR markers for fruit astringency type in crosses of ‘Taiten’×PCNA cultivars in persimmon breeding

Cited by 14 publications

References 23 publications

Persimmon breeding in Japan for pollination-constant non-astringent (PCNA) type with marker-assisted selection

Persimmon breeding in Japan for pollination-constant non-astringent (PCNA) type with marker-assisted selection

Efficiency of Sequence Characterized Amplified Region Markers for Selecting Non-Astringent Persimmon (Diospyros kaki Thunb.)

Comparative Mapping of the <i>ASTRINGENCY</i> Locus Controlling Fruit Astringency in Hexaploid Persimmon (<i>Diospyros kaki</i> Thunb.) with the Diploid <i>D. lotus</i> Reference Genome

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