Practical method combining loop-mediated isothermal amplification and bait trap to detect Pythium helicoides from hydroponic culture solutions

Miyake, Noriyuki; Nagai, Hirofumi; Kato, Shinro; Matsusaki, Masashi; Fukuta, Shiro; Takahashi, Reiko; Suzuki, Ryoji; Ishiguro, Yasushi

doi:10.1007/s10327-016-0691-6

Cited by 6 publications

(1 citation statement)

References 18 publications

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“…The first study on the detection of plant diseases was published by Fukuta et al nearly two decades ago, in 2003 [28]. The study concerned the Japanese yam mosaic virus (JYMV) and was conducted using RT-LAMP, which allows for quick, simple, and very sensitive identification of the RNA of plant viruses, as well as viroids, fungi, bacteria, and oomycetes [15,26,[29][30][31][32][33][34][35]. The speed and specificity of LAMP-based tests mean that they can be performed even on-site to roll back a contaminated product immediately and prevent the pathogen from spreading.…”

Section: Detection Of Plant Pathogensmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP): The Better Sibling of PCR?

Soroкa

Wasowicz

Rymaszewska

2021

Cells

229

100

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In 1998, when the PCR technique was already popular, a Japanese company called Eiken Chemical Co., Ltd. designed a method known as the loop-mediated isothermal amplification of DNA (LAMP). The method can produce up to 109 copies of the amplified DNA within less than an hour. It is also highly specific due to the use of two to three pairs of primers (internal, external, and loop), which recognise up to eight specific locations on the DNA or RNA targets. Furthermore, the Bst DNA polymerase most used in LAMP shows a high strand displacement activity, which eliminates the DNA denaturation stage. One of the most significant advantages of LAMP is that it can be conducted at a stable temperature, for instance, in a dry block heater or an incubator. The products of LAMP can be detected much faster than in standard techniques, sometimes only requiring analysis with the naked eye. The following overview highlights the usefulness of LAMP and its effectiveness in various fields; it also considers the superiority of LAMP over PCR and presents RT-LAMP as a rapid diagnostic tool for SARS-CoV-2.

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