Practical Method for the Detection of Tetracyclines in Honey by HPLC and Derivative UV-Vis Spectra

Abstract

A technique to detect residual tetracyclines (TCs) in honey by HPLC and derivative UV-Vis spectra is examined in this study. Chromatograms of honey products often experience overlapped peaks and sloping background due to similar retention properties of components or to the presence of impurities. The derivatives provide resolution of overlapped peaks in an UV-Vis spectrum and eventually bring chromatograms that show pure elution behavior of TCs. Calibration models for oxytetracycline (OTC), tetracycline (TC) and chlortetracycline (CTC) are built out of the derivative spectra of standard solutions of honey and their prediction abilities are examined. The results show that the calibration models for OTC, TC and CTC can yield sufficient levels for the determination. The technique reported here may be effectively used for quantitative determination of TCs.

Tetracyclines Residues in Honey

“…Different analytical methods are reported for the determination TC residues in honey. These include HPLC with UV detection [33][34][35][36][37][38][39][40], HPLC with fluorescence detection [41,42] liquid chromatography with mass spectrometry [43,44]. Nowadays, liquid chromatography coupled with mass spectrometry and tandem mass spectrometry seem to be the techniques of choice for analysis of these groups of antibiotics [45].…”

Residue Determination in Honey

“…Accordingly, European Union MRLs in food are established as sum of tetracycline and its epimer, that is, tetracycline and epi-tetracycline, oxytetracycline and epi-oxytetracycline, chlortetracycline and epi-chlortetracycline [1]. Table 10 [80][81][82][83][84][85][86][87][88], besides the classical reversed-phase solid-phase extraction cartridges (phenyl, Oasis HLB, Strata-X and C18), tetracyclines can be selectively purified applying a particular type of solid-phase extraction, that is, metal chelate affinity chromatography (MCAC) [82,86]. As mentioned before for quinolones (Section 5.6), MCAC exploits tetracycline metal complexing properties to allow for additional clean-up.…”

A Simple Colorimetric Analytical Assay for the Determination of Tetracyclines Based on In-situ Generation of Gold Nanoparticles Coupling with a Gold Staining Technique

“…Long-term and overdosage use of TCs causes damage to the liver and kidney, gastrointestinal disorder, and bone deposition. 2 Nowadays, TCs are also employed in animal husbandry to promote the growth of animals, thus, may cause undesirable residues in food and environment. 3 The residue of TCs can accumulate in human body via the food chain, which may induce antibiotic resistance cases.…”