Practical Points Regarding Routine Determination of γ -Glutamyl Transferase (γ-GT) in Serum with a Kinetic Method at 37°C

Huseby, Nils‐Erik; Strømme, J H

doi:10.3109/00365517409049892

Cited by 78 publications

(41 citation statements)

References 14 publications

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“…In the supernatant from each sample, an assay was made in duplicate of both the dehydrogenase activity (XDH) and the oxidase activity (XO) of the enzyme xanthine oxidoreductase (XOR), both according to the method of Benboubetra, Baghiani, Atmani, and Harrison (2004), and of the gammaglutamyltranspeptidase (g-GT) alkaline phosphatase (AP), and 5 0 nucleotidase (5 0 -N) activities, according to the methods of Bergmeyer (1983), Huseby and Strömme (1974) and Fini, Camici, Minelli, Floridi, and Ipata (1986), respectively.…”

Section: Extraction Mfgm Proteins and Enzyme Assaysmentioning

confidence: 99%

Fat globule membranes in ewes' milk: The main enzyme activities during lactation

Martini

Altomonte

Pesi

et al. 2013

International Dairy Journal

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Section: Extraction Mfgm Proteins and Enzyme Assaysmentioning

confidence: 99%

Fat globule membranes in ewes' milk: The main enzyme activities during lactation

Martini

Altomonte

Pesi

et al. 2013

International Dairy Journal

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“…However, the fact that plasma isoprostanes are elevated not only in experimental ethanol-induced liver damage (not shown) generally recognized as an oxidative stress injury 60 but also in alcoholic patients [61][62][63][64] lends some support to such a possibility. Finally, thiacetamide, which also induces an increased level of plasma isoprostanes, 18 has long been known as a cirrhotic drug.…”

mentioning

confidence: 98%

F2-isoprostanes stimulate collagen synthesis in activated hepatic stellate cells: a link with liver fibrosis?

Comporti

Arezzini

Signorini

et al. 2005

Laboratory Investigation

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Carbon tetrachloride (CCl 4 )-induced hepatic fibrosis has been considered to be linked to oxidative stress and mediated by aldehydic lipid peroxidation products. In the present study, we investigated whether collagen synthesis is induced by F 2 -isoprostanes, the most proximal products of lipid peroxidation and known mediators of important biological effects. By contrast with aldehydes, F 2 -isoprostanes act through receptors able to elicit definite signal transduction pathways. In a rat model of CCl 4 -induced hepatic fibrosis, plasma F 2 -isoprostanes were markedly elevated for the entire experimental period; hepatic collagen content also increased. When hepatic stellate cells (HSCs) from normal liver were cultured with F 2 -isoprostanes in the concentration range found in the in vivo studies (10 À9 -10 À8 M), a striking increase in DNA synthesis (reversed by the thromboxane A 2 antagonist SQ 29 548), in cell proliferation and in collagen synthesis was observed. Total collagen content was similarly increased. Moreover, F 2 -isoprostanes markedly increased the production of transforming growth factor-b1 by U937 cells, considered a model of liver macrophages. The data provide evidence for the possibility that F 2 -isoprostanes generated by lipid peroxidation in hepatocytes mediate HSC proliferation and collagen production seen in hepatic fibrosis.

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“…Enzyme activity was measured spectrophotometrically according to Husby and Stromme [1974] with modifications as described elsewhere [Kretzschmar and Klinger, 1989], Protein was determined with the biuret reaction using bovine scrum albumin as stan dard.…”

Section: Determination O F Ggt Activitymentioning

confidence: 99%

Perinatal Activity of Hepatic Gamma-Glutamyltranspeptidase in Rats from Large and Small Litters

Glöckner¹,

Kretzschmar²

1989

Neonatology

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In rat pups, the activity of hepatic gamma-glutamyltranspeptidase (GGT) is not influenced by the process of labor. Maximum activities are observed prenatally occurring at the 21st day of gestation in fetuses from normal, large litters, but at the 20th day of gestation in fetuses from experimentally reduced litters (2 fetuses per litter). This different developmental pattern may depend on processes of fetal maturation rather than on processes of luteolysis.

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Practical Points Regarding Routine Determination of γ -Glutamyl Transferase (γ-GT) in Serum with a Kinetic Method at 37°C

Cited by 78 publications

References 14 publications

Fat globule membranes in ewes' milk: The main enzyme activities during lactation

Fat globule membranes in ewes' milk: The main enzyme activities during lactation

F2-isoprostanes stimulate collagen synthesis in activated hepatic stellate cells: a link with liver fibrosis?

Perinatal Activity of Hepatic Gamma-Glutamyltranspeptidase in Rats from Large and Small Litters

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