The aim of this study was to investigate the separate and combined effects of precooling and warm-up on a subsequent cycling time trial in a hot environment. Nine healthy men (mean±SD age=24±5 years; body mass=74.7±4.5 kg; height=171.4±7.7 cm; body fat=12.9±5.2%) completed 3 simulated 16.1-km time trials on a cycle ergometer in a hot environment (~33 °C, 45% relative humidity) after: 1) 20 min of fluid ingestion (10 °C ) followed by 30 min of ice-slurry ingestion (-1 °C ) coupled with ice-vest (PREC), 2) 30 min of ice-slurry ingestion coupled with ice-vest followed by 20 min of warm-up including ice-slurry and ice-vest (COMBO), 3) 30 min of fluid ingestion (10 °C) followed by 20 min of warm-up (WU). At baseline, rectal temperature (T re ), mean skin temperature ( sk ), and mean body temperature ( b ) were similar among treatments (all P>0.05). After treatment administration and before the start of the time trial, T re was lower in PREC (36.1±0.3) and COMBO (36.9±0.4) compared to WU (37.6±0.2) (all P<0.05). sk and b were all lower in PREC than COMBO and WU, and were lower in COMBO than WU (all P<0.05). T re remained lower in PREC than WU throughout exercise and was lower in PREC than COMBO for the first 12 km (all P<0.01), while T re in COMBO remained lower than WU for the first 4 km. sk during PREC was lower than COMBO at 4 and 8 km and lower than WU at 0 and 4 km, while during COMBO it was lower than WU at 0 and 4 km (all P<0.05). Heart rate (HR) at baseline was lower in PREC than COMBO and WU (68±10, 106±12, 101±13 beats/min, respectively; P<0.001). During exercise, HR increased similarly among all treatments throughout exercise (all P>0.05). Local sweat rate (SR) was lower in PREC than COMBO and WU for the first 4 km (P < iv ACKNOWLEDGMENTS All praise is due to Allah, the entirely merciful; I thank him and praise him. He is the one who protects and guards me in the days when I work, and in the nights when I sleep. He amply bestowed his favors and bounties upon me, and gave me from all I asked of him, and if I should count his favors, surely I could not enumerate.Then, I would like to express my deepest gratitude to my advisor Dr. Jonathan Wingo for his guidance, knowledge, patience, diligence, and dedication throughout this dissertation stages.He was very generous in teaching the procedures of research, from how to professionally use the laboratory equipment, to how to write a solid work of research. He has been sincere, respectful, and hardworking. I am also thankful to my dissertation committee, Dr. Philip Bishop, Dr. Mark Richardson, Dr. Edward Mansfield, and Dr. John Vincent. Their inputs, suggestions, and recommendations were very effective in directing the research into the right way. Also I thank them for their cooperation and sacrifices to finish this dissertation successfully.