Practical use of chemical probes for reactive oxygen species produced in biological systems by γ-irradiation

Lee, Min Hee; Moon, Yu Ran; Chung, Byung Yeoup; Kim, Jae-Sung; Lee, Kang-Soo; Cho, Jae-Young; Kim, Jin Hong

doi:10.1016/j.radphyschem.2009.03.001

Cited by 35 publications

(14 citation statements)

References 21 publications

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“…Also in this case, we have to underline the fact that the HDR treatments induced an enhanced antioxidant response. The occurrence of oxidative stress and activation of antioxidant mechanisms were separately reported in response to γ -rays and salinity stress in several other studies [11, 12, 45–47]. The extent of these responses is mainly correlated with the used doses.…”

Section: Discussionmentioning

confidence: 91%

Synergistic Exposure of Rice Seeds to Different Doses ofγ-Ray and Salinity Stress Resulted in Increased Antioxidant Enzyme Activities and Gene-Specific Modulation of TC-NER Pathway

Macovei

Garg

Raikwar

et al. 2014

BioMed Research International

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Recent reports have underlined the potential of gamma (γ)-rays as tools for seed priming, a process used in seed industry to increase seed vigor and to enhance plant tolerance to biotic/abiotic stresses. However, the impact of γ-rays on key aspects of plant metabolism still needs to be carefully evaluated. In the present study, rice seeds were challenged with different doses of γ-rays and grown in absence/presence of NaCl to assess the impact of these treatments on the early stages of plant life. Enhanced germination efficiency associated with increase in radicle and hypocotyl length was observed, while at later stages no increase in plant tolerance to salinity stress was evident. APX, CAT, and GR were enhanced at transcriptional level and in terms of enzyme activity, indicating the activation of antioxidant defence. The profiles of DNA damage accumulation were obtained using SCGE and the implication of TC-NER pathway in DNA damage sensing and repair mechanisms is discussed. OsXPB2, OsXPD, OsTFIIS, and OsTFIIS-like genes showed differential modulation in seedlings and plantlets in response to γ-irradiation and salinity stress. Altogether, the synergistic exposure to γ-rays and NaCl resulted in enhanced oxidative stress and proper activation of antioxidant mechanisms, thus being compatible with plant survival.

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Section: Discussionmentioning

confidence: 91%

Synergistic Exposure of Rice Seeds to Different Doses ofγ-Ray and Salinity Stress Resulted in Increased Antioxidant Enzyme Activities and Gene-Specific Modulation of TC-NER Pathway

Macovei

Garg

Raikwar

et al. 2014

BioMed Research International

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“…direct deposit of ionizing energy resulting in DNA double strand breaks (DSBs), and indirect action of reactive oxygen species (e.g. H 2 O 2 , ⦁ OH, 2 O • ) generated by ionization-induced water radiolysis (Lee et al, 2009;Lagoda, 2012), which could result in damage at the nucleotide level. Hence it is not unexpected to detect SBS mutations in the P 2 M 2 plants.…”

Section: Type Of γ Irradiation-induced Mutationsmentioning

confidence: 99%

Frequency and type of inheritable mutations induced by γ rays in rice as revealed by whole genome sequencing

Zheng

Hairui

et al. 2016

J. Zhejiang Univ. Sci. B

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Mutation breeding is based on the induction of genetic variations; hence knowledge of the frequency and type of induced mutations is of paramount importance for the design and implementation of a mutation breeding program. Although γ ray irradiation has been widely used since the 1960s in the breeding of about 200 economically important plant species, molecular elucidation of its genetic effects has so far been achieved largely by analysis of target genes or genomic regions. In the present study, the whole genomes of six γ-irradiated M 2 rice plants were sequenced; a total of 144-188 million high-quality (Q>20) reads were generated for each M 2 plant, resulting in genome coverage of >45 times for each plant. Single base substitution (SBS) and short insertion/deletion (Indel) mutations were detected at the average frequency of 7.5×10 −6 -9.8×10 −6 in the six M 2 rice plants (SBS being about 4 times more frequent than Indels). Structural and copy number variations, though less frequent than SBS and Indel, were also identified and validated. The mutations were scattered in all genomic regions across 12 rice chromosomes without apparent hotspots. The present study is the first genome-wide single-nucleotide resolution study on the feature and frequency of γ irradiation-induced mutations in a seed propagated crop; the findings are of practical importance for mutation breeding of rice and other crop species.

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“…In a spontaneous reaction catalyzed by ferrous iron (Fenton reaction), H 2 O 2 can be converted to highly reactive ·OH (Lisher and Giedroc, 2013). Intracellular ·OH is trapped by ethanol/4-POBN, forming hydroxyethyl-POBN adducts, which can be detected by electron paramagnetic resonance (EPR) spin trapping (Lee et al, 2009). The relative intensity of the six-line EPR signal, which is characteristically exhibited by POBN radical adducts, was markedly decreased in Ec-PprM compared with Ec-pASK (Figure 3), demonstrating that the ·OH level is lower in Ec-PprM than in Ec-pASK.…”

Section: Resultsmentioning

confidence: 99%

“…The amounts of hydroxyl radical (·OH) in Ec-PprM and Ec-pASK cells were detected using electron paramagnetic resonance (EPR) spectroscopy with the specific probe ethanol/a-(4-pyridyl-1-oxide)-N-tert-butylnitrone (4-POBN) as previously described (Lee et al, 2009). Following 2 h incubation with AHT (200 ng/ml), the cells were washed twice with ice-cold Chelex-treated Hank's balanced salt solution (HBSS) and resuspended in ice-cold HBSS.…”

Section: Methodsmentioning

confidence: 99%

PprM, a Cold Shock Domain-Containing Protein from Deinococcus radiodurans, Confers Oxidative Stress Tolerance to Escherichia coli

et al. 2017

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Escherichia coli is a representative microorganism that is frequently used for industrial biotechnology; thus its cellular robustness should be enhanced for the widespread application of E. coli in biotechnology. Stress response genes from the extremely radioresistant bacterium Deinococcus radiodurans have been used to enhance the stress tolerance of E. coli. In the present study, we introduced the cold shock domain-containing protein PprM from D. radiodurans into E. coli and observed that the tolerance to hydrogen peroxide (H2O2) was significantly increased in recombinant strains (Ec-PprM). The overexpression of PprM in E. coli elevated the expression of some OxyR-dependent genes, which play important roles in oxidative stress tolerance. Particularly, mntH (manganese transporter) was activated by 9-fold in Ec-PprM, even in the absence of H2O2 stress, which induced a more than 2-fold increase in the Mn/Fe ratio compared with wild type. The reduced production of highly reactive hydroxyl radicals (·OH) and low protein carbonylation levels (a marker of oxidative damage) in Ec-PprM indicate that the increase in the Mn/Fe ratio contributes to the protection of cells from H2O2 stress. PprM also conferred H2O2 tolerance to E. coli in the absence of OxyR. We confirmed that the H2O2 tolerance of oxyR mutants reflected the activation of the ycgZ-ymgABC operon, whose expression is activated by H2O2 in an OxyR-independent manner. Thus, the results of the present study showed that PprM could be exploited to improve the robustness of E. coli.

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Practical use of chemical probes for reactive oxygen species produced in biological systems by γ-irradiation

Cited by 35 publications

References 21 publications

Synergistic Exposure of Rice Seeds to Different Doses ofγ-Ray and Salinity Stress Resulted in Increased Antioxidant Enzyme Activities and Gene-Specific Modulation of TC-NER Pathway

Synergistic Exposure of Rice Seeds to Different Doses ofγ-Ray and Salinity Stress Resulted in Increased Antioxidant Enzyme Activities and Gene-Specific Modulation of TC-NER Pathway

Frequency and type of inheritable mutations induced by γ rays in rice as revealed by whole genome sequencing

PprM, a Cold Shock Domain-Containing Protein from Deinococcus radiodurans, Confers Oxidative Stress Tolerance to Escherichia coli

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