Praxis der präparativen Free-Flow-EIektrophorese

Wagner, H.; Kuhn, Reinhard; Hoffstetter, S.

doi:10.1007/978-3-642-73622-3_6

Cited by 10 publications

(2 citation statements)

References 152 publications

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“…The apparatus was developed by Wagner et al [9] and can accept some liters of analyte solution within a reasonable time. In this technique, a higher conductivity is used to focus the ions in the boundary zone between the zones aA and HC (see Fig.…”

Section: Continuous Electrophoretic Ion Focusingmentioning

confidence: 99%

Complexation behaviour of neptunium with humic acid

Seibert¹,

Mansel²,

Marquardt³

et al. 2001

Radiochimica Acta

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The complexation of Np(V) with humic acid (HA) was studied under environmental conditions (pH ≥ 6, concentration of HA 1-150 mg/L) over a wide range of metal ion concentrations (10 −14 to 10 −4 mol/L). The experimental methods used for the determination of the complexation constants were continuous electrophoretic ion focusing, anion exchange, and ultrafiltration in combination with radiometric measurements, and, as a direct speciation method, UV/Visspectroscopy. The interaction between Np(V) and humic acid has been described with the charge neutralization model including a correction for different loading capacities (LC) which makes possible the comparison of results obtained at various experimental conditions. Furthermore, the degree of deprotonation has also been used to calculate the complexation constants. The complexation constants calculated for Np(V) with the LC-concept vary with the metal ion concentration in the range between 10 −14 and 10 −4 mol/L. At metal concentrations below 10 −11 mol/L, a complexation constant log β LC = 5.0 ± 0.3 was obtained. In the concentration range 10 −7 to 5 × 10 −4 mol/L, the log β LC values decrease continuously to 3.7 ± 0.4 (10 −5 -5 × 10 −4 mol/L). The latter is in agreement with direct measurements (UV/Vis-spectroscopy) at this concentration and data from the literature.

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Section: Continuous Electrophoretic Ion Focusingmentioning

confidence: 99%

Complexation behaviour of neptunium with humic acid

Seibert¹,

Mansel²,

Marquardt³

et al. 2001

Radiochimica Acta

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“…FFZE was the most popular separation mode utilized for isolation of enzyme. The detail of principle can be found in [38]. However, these works [31][32][33][34][35][36][37] concerned large-scale separation rather than the micropreparation of enzyme.…”

Section: Introductionmentioning

confidence: 99%

Reassemblable quasi‐chip free‐flow electrophoresis with simple heating dispersion for rapid micropreparation of trypsin in crude porcine pancreatin

et al. 2011

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An increasing number of small biosamples (e.g. proteins and enzymes) need micropreparation in lab. However, neither large-scale free-flow electrophoresis (LS-FFE) nor chip FFE (C-FFE) could fit the growing demands. Herein, a simple quasi-chip FFE (QC-FFE) was constructed. In contrast to C-FFE, the features of QC-FFE are as follows: (i) its separation chamber is reassemblable and rewashable avoiding discard of C-FFE due to blockage of solute precipitation in chamber; (ii) its chamber size is 45 mm × 30 mm × (80-500) μm (108-654 μL volume) having function of micropreparation; (iii) there are up to 16 outlets in QC-FFE bestowing fine fraction for micropurification. The QC-FFE was used for the micropurification of model enzyme of self-digestible trypsin in crude pancreatin. Under the given conditions, the purification factor of enzyme was 11.7, the specific activity reached 6236 U/mg, the run time for 19 μL sample purification was 45 s and the throughput of trypsin was 3.34 mg/h, and the yield of pure trypsin was 55.2%. All of the results show the feasibility of enzyme micropreparation via QC-FFE. The developed device and procedure have potential use to other micropurification of protein or peptide sample.

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2008

Proteomics in Practice

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Praxis der präparativen Free-Flow-EIektrophorese

Cited by 10 publications

References 152 publications

Complexation behaviour of neptunium with humic acid

Complexation behaviour of neptunium with humic acid

Reassemblable quasi‐chip free‐flow electrophoresis with simple heating dispersion for rapid micropreparation of trypsin in crude porcine pancreatin

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