Pre‐analytical stability of coagulation parameters in plasma stored at room temperature

Linskens, Eleni A.; Devreese, Katrien

doi:10.1111/ijlh.12784

Cited by 24 publications

(42 citation statements)

References 20 publications

(106 reference statements)

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“…According to The Clinical and Laboratory Standards Institute (CLSI), the French Study Group on Hemostasis and Thrombosis (GFHT) and European Concerted Action on Thrombosis (ECAT), specimens kept at room temperature (15°C-25°C) for routine haemostasis tests or determination of coagulation factors should be analyzed within 4 hours of collection, except for the quick time (TQ), which has a stability of up to 24 hours, and tests to monitor treatment with unfractionated heparin, for which the delay should not exceed 2 hours [17,18]. Several studies have demonstrated longer stability for many hemostasis parameters.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have demonstrated longer stability for many hemostasis parameters. This can be interesting, for example, when additional coagulation tests are requested or when laboratories must subcontract coagulation tests to a laboratory remote from the sampling site [2,[17][18][19]. However, further studies are needed to confirm the validity of this eventuality.…”

Section: Discussionmentioning

confidence: 99%

“…The rate of under-filling tubes from services (28%) was higher than that of tubes received from the blood drawing room (19%). Our rates are high by comparing them to the rates of similar studies [5][6][7][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Pre-analytical Phase in Hemostasis: The Main Anomalies and Means to Correct Them

Siham¹,

Ikhlas²,

Raihane³

et al. 2019

AJLM

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Pre-analytical errors still represent nearly 70% of all errors occurring in the laboratory, constituting a danger, a waste of time and an additional cost to the patient. The control of the different components of the pre-analytical step is important for the validity of the hemostasis exploration tests. The purpose of our work is to identify the main anomalies of the pre-analytical phase in hemostasis and to propose the means to correct them. We conducted a prospective and descriptive study on the pre-analytical phase of hemostasis. It was in the form of a survey, identifying the main errors related to this phase. It was performed at the Hematology laboratory of the Avicenna Military Hospital of Marrakech and spread over a period of 4 weeks. Our investigation took place at the hemostasis room, which received the collection tubes from the various hospital departments and the blood drawing room (for non-hospitalized patients). The hemostasis room received 400 prescription cards and their corresponding tubes. The parameters related to the prescription file: full name and gender of the patients, were mentioned on all the cards received and they were in conformity with those marked on the corresponding tube. The age of the patients and their clinical and therapeutic informations were mentioned in 73% and 13% of the exam requests, respectively. For the pre-analytical hemostasis parameters related to the blood collection: 63% of samples were taken at the laboratory's blood drawing room, while 37% came from the various hospital departments. Time of the realization of the samples was not mentioned on the cards or on the labels of the tubes. The anticoagulant used for all samples was sodium citrate at a concentration of 3.8%. The filling of the tubes was noncompliant in 22.25%. Registration and triage of the tubes systems were manual. Centrifugation was carried out at a rotation speed of 5000 G for 5 minutes and at a temperature set at 22°C. Hemolyzed samples accounted for 3% of the tubes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Pre-analytical Phase in Hemostasis: The Main Anomalies and Means to Correct Them

Siham¹,

Ikhlas²,

Raihane³

et al. 2019

AJLM

View full text Add to dashboard Cite

show abstract

“…The CLSI guideline is very strict, demanding all coagulation tests to be centrifuged and analyzed within 4 h, except PT-INR within 24 h and APTT with unfractionated heparin treatment within 2 h, but also states that longer storage time could be used if this is validated in one's own laboratory [7]. There are studies indicating that both PT-INR and fibrinogen are stable for 48 h and APTT for 24 h, both in citrated blood and plasma [5,6]. PT-INR and fibrinogen have even been shown to be stable for up to 72 h [27] and 7 days [28], respectively.…”

Section: Sample Stability and Storagementioning

confidence: 99%

“…In the case of insufficient mixing a clot could be formed in the sample [3]. Underfilled tubes or prolonged storage before testing may lead to a decrease of coagulation factor activities or altered impression of the anticoagulant effect in a monitoring situation [4][5][6][7]. In these circumstances, results would represent the situation in vitro and not in vivo.…”

Section: Introductionmentioning

confidence: 99%

Pre-analytical practices for routine coagulation tests in European laboratories. A collaborative study from the European Organisation for External Quality Assurance Providers in Laboratory Medicine (EQALM)

Kristoffersen

Stavelin

Ajzner³

et al. 2019

Clinical Chemistry and Laboratory Medicine (CCLM)

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Background Correct handling and storage of blood samples for coagulation tests are important to assure correct diagnosis and monitoring. The aim of this study was to assess the pre-analytical practices for routine coagulation testing in European laboratories. Methods In 2013–2014, European laboratories were invited to fill in a questionnaire addressing pre-analytical requirements regarding tube fill volume, citrate concentration, sample stability, centrifugation and storage conditions for routine coagulation testing (activated partial thromboplastin time [APTT], prothrombin time in seconds [PT-sec] and as international normalised ratio [PT-INR] and fibrinogen). Results A total of 662 laboratories from 28 different countries responded. The recommended 3.2% (105–109 mmol/L) citrate tubes are used by 74% of the laboratories. Tube fill volumes ≥90% were required by 73%–76% of the laboratories, depending upon the coagulation test and tube size. The variation in centrifugation force and duration was large (median 2500 g [10- and 90-percentiles 1500 and 4000] and 10 min [5 and 15], respectively). Large variations were also seen in the accepted storage time for different tests and sample materials, for example, for citrated blood at room temperature the accepted storage time ranged from 0.5–72 h and 0.5–189 h for PT-INR and fibrinogen, respectively. If the storage time or the tube fill requirements are not fulfilled, 72% and 84% of the respondents, respectively, would reject the samples. Conclusions There was a large variation in pre-analytical practices for routine coagulation testing in European laboratories, especially for centrifugation conditions and storage time requirements.

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Assessing Blood Clotting and Coagulation Factors in Mice

et al. 2019

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The mammalian blood coagulation system was designed to restrict blood loss due to injury as well as keep the blood fluid within the blood vessels of the organism. Blood coagulation activity in inbred mouse strains varies widely among strains, suggesting that many genomic variants affect hemostasis. Some of these molecules have been discovered and characterized; however, many are still unknown. Genetically modified mouse technologies are providing a plethora of new mouse models for investigating the regulation of blood coagulation. Here we provide a protocol for the tail bleeding time as a primary assessment of in vivo blood coagulation, as well as in vitro methods such as the prothrombin time, activated partial thromboplastin time, and thrombin generation assay. We also provide protocols for the assessment of the activities of specific known factors involved in blood coagulation. © 2019 by John Wiley & Sons, Inc.

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Pre‐analytical stability of coagulation parameters in plasma stored at room temperature

Cited by 24 publications

References 20 publications

Pre-analytical Phase in Hemostasis: The Main Anomalies and Means to Correct Them

Pre-analytical Phase in Hemostasis: The Main Anomalies and Means to Correct Them

Pre-analytical practices for routine coagulation tests in European laboratories. A collaborative study from the European Organisation for External Quality Assurance Providers in Laboratory Medicine (EQALM)

Assessing Blood Clotting and Coagulation Factors in Mice

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