Pre- and Peri-/Post-Compaction Follistatin Treatment Increases In Vitro Production of Cattle Embryos

Guo, Zhenhua; Rajput, Sandeep; Folger, Joseph K.; Liu, Di; Knott, Jack H.; Smith, George W.

doi:10.1371/journal.pone.0170808

Cited by 10 publications

(6 citation statements)

References 34 publications

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“…Albumin has been found to increase blastocyst development in individual culture of bovine embryos [ 117 ]. Follistatin has been shown to be important for bovine early embryo development [ 118 , 119 ]. Complement C5a has been shown to support human embryonic stem cell pluripotency even in the absence of other growth factors [ 120 ] ( S1 Fig ).…”

Section: Resultsmentioning

confidence: 99%

Profiling of proteins secreted in the bovine oviduct reveals diverse functions of this luminal microenvironment

et al. 2017

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The oviductal microenvironment is a site for key events that involve gamete maturation, fertilization and early embryo development. Secretions into the oviductal lumen by either the lining epithelium or by transudation of plasma constituents are known to contain elements conducive for reproductive success. Although previous studies have identified some of these factors involved in reproduction, knowledge of secreted proteins in the oviductal fluid remains rudimentary with limited definition of function even in extensively studied species like cattle. In this study, we used a shotgun proteomics approach followed by bioinformatics sequence prediction to identify secreted proteins present in the bovine oviductal fluid (ex vivo) and secretions from the bovine oviductal epithelial cells (in vitro). From a total of 2087 proteins identified, 266 proteins could be classified as secreted, 109 (41%) of which were common for both in vivo and in vitro conditions. Pathway analysis indicated different classes of proteins that included growth factors, metabolic regulators, immune modulators, enzymes, and extracellular matrix components. Functional analysis revealed mechanisms in the oviductal lumen linked to immune homeostasis, gamete maturation, fertilization and early embryo development. These results point to several novel components that work together with known elements mediating functional homeostasis, and highlight the diversity of machinery associated with oviductal physiology and early events in cattle fertility.

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Section: Resultsmentioning

confidence: 99%

Profiling of proteins secreted in the bovine oviduct reveals diverse functions of this luminal microenvironment

et al. 2017

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“…We previously demonstrated that follistatin treatment had a positive impact on increasing TE cell numbers in bovine embryos (Lee et al, 2009; Zhenhua et al, 2017). Importantly, in the present study, we do not believe that increased TE cell numbers dramatically affected the observed methylation changes on CDX2 regulatory regions.…”

Section: Discussionmentioning

confidence: 97%

“…Follistatin transcripts were higher in BCB positive (BCB+) high‐quality oocytes compared to BCB− oocytes (Ashry et al, 2015). Functional studies revealed that maternally derived follistatin is essential for early development in bovine preimplantation embryos and that exogenous follistatin supplementation during the first 72 hr of in vitro embryo culture improves the developmental capacity of bovine (Ashry et al, 2015; Ashry, Lee, Folger, Rajput, & Smith, 2018; Ashry, Rajput, et al, 2018; Lee, Bettegowda, Wee, Ireland, & Smith, 2009; Patel et al, 2007; Zhenhua et al, 2017) and Rhesus macaque embryos (VandeVoort, Mtango, Lee, Smith, & Latham, 2009). Interestingly, in vitro‐produced (IVP) blastocysts derived from embryos treated with follistatin exhibit higher trophectoderm (TE) cell numbers and increased expression of caudal‐type homeobox 2 ( CDX2 ) messenger RNA (mRNA; Lee et al, 2009; Zhenhua et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Follistatin treatment modifies DNA methylation of the CDX2 gene in bovine preimplantation embryos

Ashry

Rajput

Folger

et al. 2020

Molecular Reproduction Devel

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CDX2 plays a crucial role in the formation and maintenance of the trophectoderm epithelium in preimplantation embryos. Follistatin supplementation during the first 72 hr of in vitro culture triggers a significant increase in blastocyst rates, CDX2 expression, and trophectoderm cell numbers. However, the underlying epigenetic mechanisms by which follistatin upregulates CDX2 expression are not known. Here, we investigated whether stimulatory effects of follistatin are linked to alterations in DNA methylation within key regulatory regions of the CDX2 gene. In vitro-fertilized (IVF) zygotes were cultured with or without 10 ng/ml of recombinant human follistatin for 72 hr, then cultured without follistatin until Day 7. The bisulfitesequencing analysis revealed differential methylation (DM) at specific CpG sites within the CDX2 promoter and intron 1 following follistatin treatment. These DM CpG sites include five hypomethylated sites at positions −1384, −1283, −297, −163, and −23, and four hypermethylated sites at positions −1501, −250, −243, and +20 in the promoter region. There were five hypomethylated sites at positions +3060, +3105, +3219, +3270, and +3545 in intron 1. Analysis of transcription factor binding sites using MatInspector combined with a literature search revealed a potential association between differentially methylated CpG sites and putative binding sites for key transcription factors involved in regulating CDX2 expression. The hypomethylated sites are putative binding sites for FXR, STAF, OCT1, KLF, AP2 family, and P53 protein, whereas the hypermethylated sites are putative binding sites for NRSF. Collectively, our results suggest that follistatin may increase CDX2 expression in early bovine embryos, at least in part, by modulating DNA methylation at key regulatory regions.

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“…CDX2 is a transcription factor that is localized in the nucleus of the trophectoderm [ 58 ]. Studies have shown that CDX2 is essential for early development and gene expression involved in the differentiation of inner cell mass and trophectoderm lineages in cattle embryos [ 30 , 59 ]. KDM6B , also known as JMJD3 , is a histone demethylase expressed in bovine cleavage embryos [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

Assessing genomic diversity and signatures of selection in Pinan cattle using whole-genome sequencing data

Zhang

Yao

et al. 2022

BMC Genomics

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Background Crossbreeding is an important way to improve production beef cattle performance. Pinan cattle is a new hybrid cattle obtained from crossing Piedmontese bulls with Nanyang cows. After more than 30 years of cross-breeding, Pinan cattle show a variety of excellent characteristics, including fast growth, early onset of puberty, and good meat quality. In this study, we analyzed the genetic diversity, population structure, and genomic region under the selection of Pinan cattle based on whole-genome sequencing data of 30 Pinan cattle and 169 published cattle genomic data worldwide. Results Estimating ancestry composition analysis showed that the composition proportions for our Pinan cattle were mainly Piedmontese and a small amount of Nanyang cattle. The analyses of nucleotide diversity and linkage disequilibrium decay indicated that the genomic diversity of Pinan cattle was higher than that of European cattle and lower than that of Chinese indigenous cattle. De-correlated composite of multiple selection signals, which combines four different statistics including θπ, CLR, FST, and XP-EHH, was computed to detect the signatures of selection in the Pinan cattle genome. A total of 83 genes were identified, affecting many economically important traits. Functional annotation revealed that these selected genes were related to immune (BOLA-DQA2, BOLA-DQB, LSM14A, SEC13, and NAALADL2), growth traits (CYP4A11, RPL26, and MYH10), embryo development (REV3L, NT5E, CDX2, KDM6B, and ADAMTS9), hornless traits (C1H21orf62), and climate adaptation (ANTXR2). Conclusion In this paper, we elucidated the genomic characteristics, ancestry composition, and selective signals related to important economic traits in Pinan cattle. These results will provide the basis for further genetic improvement of Pinan cattle and reference for other hybrid cattle related studies.

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Pre- and Peri-/Post-Compaction Follistatin Treatment Increases In Vitro Production of Cattle Embryos

Cited by 10 publications

References 34 publications

Profiling of proteins secreted in the bovine oviduct reveals diverse functions of this luminal microenvironment

Profiling of proteins secreted in the bovine oviduct reveals diverse functions of this luminal microenvironment

Follistatin treatment modifies DNA methylation of the CDX2 gene in bovine preimplantation embryos

Assessing genomic diversity and signatures of selection in Pinan cattle using whole-genome sequencing data

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