1984
DOI: 10.1016/0092-8674(84)90508-7
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Pre- and post-golgi vacuoles operate in the transport of semliki forest virus membrane glycoproteins to the cell surface

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Cited by 509 publications
(410 citation statements)
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“…4 shows that the recovery of activity was progressively slowed when temperatures decreased from 37°C (FR 1/2 ϭ 1 h) to 30°C (FR 1/2 ϭ 2 h) to 22°C (FR 1/2 ϭ 3.5 h). At 15°C, which typically blocks the ER-to-Golgi transition (20), the activity was recovered partially, up to 40% of the initial level (Fig. 4a).…”
Section: Resultsmentioning
confidence: 88%
See 1 more Smart Citation
“…4 shows that the recovery of activity was progressively slowed when temperatures decreased from 37°C (FR 1/2 ϭ 1 h) to 30°C (FR 1/2 ϭ 2 h) to 22°C (FR 1/2 ϭ 3.5 h). At 15°C, which typically blocks the ER-to-Golgi transition (20), the activity was recovered partially, up to 40% of the initial level (Fig. 4a).…”
Section: Resultsmentioning
confidence: 88%
“…3a and 4 a-c). It is known that lowering the temperature can stall the ER-to-Golgi or cis-to trans-Golgi transitions (20). In either case, only limited recovery should be observed, presumably caused by the vesicles in transit between trans-Golgi and the cell surface.…”
Section: Resultsmentioning
confidence: 99%
“…Specifically, transport vesicles budding from the ER are not able to fuse with the Golgi apparatus at temperatures below 15°C, and transport beyond the TGN is inhibited at 20°C (55)(56)(57)(58). As an additional test of the proposal that GPIs are transported to the cell surface via a vesicular transport mechanism, we investigated the effect of lowered temperature on GPI transport to the cell surface.…”
Section: Fig 3 Analysis Of P10; H8 Is Mainly Located In the Mitochomentioning
confidence: 99%
“…BHK21 cells were infected with the wild type SFV as described previously [17]. Pulse labeling with [ 35 S]methionine and immunoprecipitation with SFV-E2 speci¢c antiserum were performed according to Sariola et al [14].…”
Section: Viral Infection and Pulse-chase Experimentsmentioning
confidence: 99%