Pre- and Post-Harvest Conditions Affect Polyphenol Content in Strawberry (Fragaria × ananassa)

Koyama, Ryohei; Ishibashi, Misaki; Fukuda, Itsuko; Okino, Akitoshi; Osawa, Ro; Uno, Yuichi

doi:10.3390/plants11172220

Cited by 10 publications

(10 citation statements)

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“…Changes in genes associated with phenylpropanoid metabolism confirmed previous work where strawberry fruit were stored at a much colder temperature of 0°C ( Koyama et al., 2022 ) for a longer period of time (10 days). Genes encoding coniferyl-aldehyde dehydrogenase (EC:1.2.1.68), and beta-glucosidase (EC:3.2.1.21) were up-regulated in both studies while genes encoding cinnamyl alcohol dehydrogenase (EC:1.1.1.195) were down-regulated, and peroxidase (EC:1.11.1.7) genes were both up- and down-regulated during storage.…”

Section: Discussionsupporting

confidence: 86%

“…It is also of interest that ABA related genes were only up-regulated later, perhaps when storage dehydration is acting as a trigger. A peak in ABA content was noted on day 2 of storage at 0°C (Koyama et al, 2022) and would be consistent with the subsequent activation of ABA responsive genes. The down-regulation of abiotic stress related genes later in storage is perhaps surprising although it may reflect a general shutting down of metabolism linked to senescence.…”

Section: Discussionsupporting

confidence: 66%

“…The majority of the DEGs found here associated with starch and sugar metabolism increased in expression. Starch and sugar metabolism was also an enriched pathway when strawberry fruit were exposed to 0°C for 10 days ( Koyama et al., 2022 ). Most of the enzymes encoded catalyse the production of monosaccharides: glucose, maltose, and fructose or dextrin, a breakdown product of starch.…”

Section: Discussionmentioning

confidence: 99%

“…Genes encoding coniferyl-aldehyde dehydrogenase (EC:1.2.1.68), and beta-glucosidase (EC:3.2.1.21) were up-regulated in both studies while genes encoding cinnamyl alcohol dehydrogenase (EC:1.1.1.195) were down-regulated, and peroxidase (EC:1.11.1.7) genes were both up- and down-regulated during storage. However, genes for two further enzymes: shikimate O-hydroxycinnamoyl transferase (EC:2.3.1.133) and ferulate-5-hydroxylase (EC:1.14.-.-) were only up-regulated when fruit were exposed to 0°C for 10 days ( Koyama et al., 2022 ) and not here where storage was at 8°C suggesting that genes for these enzymes are only activated at a colder storage temperature or a longer period of time. Conversely genes encoding other enzymes in this pathway, including cinnamoyl-CoA reductase (EC 1.2.1.44) were only down-regulated, and others including coniferyl-aldehyde dehydrogenase (EC 1.2.1.68), were only up-regulated here.…”

Section: Discussionmentioning

confidence: 99%

“…Treatment with phytohormones postharvest ( Chen et al., 2016 ), oF fruit stored at ambient temperature for 2 days showed that auxin delayed postharvest ripening whereas ABA accelerated it. A study of changes in polyphenol content during fruit storage at 0°C over a 10 day period ( Koyama et al., 2022 ) identified changes in expression of genes for several key enzymes in the phenylpropanoid biosynthesis pathway which may be linked to changes in polyphenol content in response to cold storage.…”

Section: Introductionmentioning

confidence: 99%

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Storage of halved strawberry fruits affects aroma, phytochemical content and gene expression, and is affected by pre-harvest factors

et al. 2023

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IntroductionStrawberry fruit are highly valued for their aroma which develops during ripening. However, they have a short shelf-life. Low temperature storage is routinely used to extend shelf-life for transport and storage in the supply chain, however cold storage can also affect fruit aroma. Some fruit continue to ripen during chilled storage; however, strawberries are a non-climacteric fruit and hence ripening postharvest is limited. Although most strawberry fruit is sold whole, halved fruit is also used in ready to eat fresh fruit salads which are of increasing consumer demand and pose additional challenges to fresh fruit storage.MethodsTo better understand the effects of cold storage, volatilomic and transcriptomic analyses were applied to halved Fragaria x ananassa cv. Elsanta fruit stored at 4 or 8°C for up to 12 days over two growing seasons.Results and discussionThe volatile organic compound (VOC) profile differed between 4 or 8°C on most days of storage. Major differences were detected between the two different years of harvest indicating that aroma change at harvest and during storage is highly dependent on environmental factors during growth. The major component of the aroma profile in both years was esters. Over 3000 genes changed in expression over 5 days of storage at 8°C in transcriptome analysis. Overall, phenylpropanoid metabolism, which may also affect VOCs, and starch metabolism were the most significantly affected pathways. Genes involved in autophagy were also differentially expressed. Expression of genes from 43 different transcription factor (TF) families changed in expression: mostly they were down-regulated but NAC and WRKY family genes were mainly up-regulated. Given the high ester representation amongst VOCs, the down-regulation of an alcohol acyl transferase (AAT) during storage is significant. A total of 113 differentially expressed genes were co-regulated with the AAT gene, including seven TFs. These may be potential AAT regulators.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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