Pre- and Post-Resection Urine Metabolic Profiles of Bladder Cancer Patients: Results of Preliminary Studies on Time Series Metabolomics Analysis

Abstract

The incidence of bladder cancer (BCa) has remained high for many years. Nevertheless, its pathomechanism has not yet been fully understood and is still being studied. Therefore, multiplatform untargeted urinary metabolomics analysis has been performed in order to study differences in the metabolic profiles of urine samples collected at three time points: before transurethral resection of bladder tumor (TURBT), the day after the procedure and two weeks after TURBT. Collected samples were analyzed with the use of high-performance liquid chromatography hyphenated with time-of-flight mass spectrometry detection (HPLC-TOF/MS) and gas chromatography coupled with triple quadrupole mass spectrometry detection (GC-QqQ/MS, in a scan mode). Levels of metabolites selected in our previous study were assessed in order to confirm their potential to differentiate the healthy and diseased samples, regardless of the risk factors and individual characteristics. Hippuric acid, pentanedioic acid and uridine confirmed their potential for sample differentiation. Based on the results of statistical analysis for the paired samples (comparison of metabolic profiles of samples collected before TURBT and two weeks after), a set of metabolites belonging to nucleotide metabolism and methylation processes was also selected. Longitudinal studies proved to be useful for the evaluation of metabolic changes in bladder cancer.

Metabolomics for the diagnosis of bladder cancer: A systematic review

“…After reviewing the titles, abstracts, and the full texts, we selected 30 studies for qualitative synthesis ( Fig. 1 ) [ [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] , [39] , [40] , [41] , [42] , [43] , [44] , [45] , [46] , [47] , [48] , [49] , [50] ].

Figure 1 Flowchart of selected studies.

…”

Serum metabolites as early detection markers of non-muscle invasive bladder cancer in Chinese patients

“…Bca is still a worldwide problem in people over the age of 50, and the incidence differs among races, with a lower morbidity but a poor survival rate in African Americans (1). The mortality rates for men and women are similar, and the development risk is four times higher in men than in women (2). The risk factors for Bca include age, race, sex, body mass index (BMI), smoking, pathogen infections, and socioeconomic status.…”

A Time-Series Metabolomic Analysis of SARS-CoV-2 Infection in a Ferret Model

“…Sphingomyelin and lysophosphatidylethanolamine were identified as putative biomarkers in the time series analysis for discriminating between multiple sclerosis patients and healthy individuals [13]. Jacyna, et al [14] investigated the urine metabolic profiles of bladder cancer patients pre-and post-resection. It was reported that hippuric acid, pentanedioic acid and uridine could potentially be used for sample differentiation [14].…”

“…Jacyna, et al [14] investigated the urine metabolic profiles of bladder cancer patients pre-and post-resection. It was reported that hippuric acid, pentanedioic acid and uridine could potentially be used for sample differentiation [14]. Huang, et al [15] analyzed the time-series lipidomics data to study the development of hepatocellular carcinoma in a hepatocarcinogenesis rat model.…”