Pre- and postmortem imaging of transplanted cells

Andrzejewska, A; Nowakowski, Adam; Janowski, Mirosław; Bulte, Jeff W.M.; Gilad, Assaf A.; Walczak, Piotr; Łukomska, Barbara

doi:10.2147/ijn.s83557

Cited by 7 publications

(3 citation statements)

References 174 publications

(173 reference statements)

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“…Labeling NSCs, and then monitoring their proliferation, migration, and integration into the tissue is possible thanks to real-time magnetic resonance imaging (MRI) ( Obenaus et al, 2011 ). Many other methods of imaging are also applicable for transplanted cadaveric stem cells ( Andrzejewska et al, 2015 ). All of them aim to assist researchers in assessing the effectiveness of the transplant.…”

Section: Limitations and Possibilitiesmentioning

confidence: 99%

Cadaveric Stem Cells: Their Research Potential and Limitations

Cieśla

Tomsia

2021

Front. Genet.

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In the era of growing interest in stem cells, the availability of donors for transplantation has become a problem. The isolation of embryonic and fetal cells raises ethical controversies, and the number of adult donors is deficient. Stem cells isolated from deceased donors, known as cadaveric stem cells (CaSCs), may alleviate this problem. So far, it was possible to isolate from deceased donors mesenchymal stem cells (MSCs), adipose delivered stem cells (ADSCs), neural stem cells (NSCs), retinal progenitor cells (RPCs), induced pluripotent stem cells (iPSCs), and hematopoietic stem cells (HSCs). Recent studies have shown that it is possible to collect and use CaSCs from cadavers, even these with an extended postmortem interval (PMI) provided proper storage conditions (like cadaver heparinization or liquid nitrogen storage) are maintained. The presented review summarizes the latest research on CaSCs and their current therapeutic applications. It describes the developments in thanatotranscriptome and scaffolding for cadaver cells, summarizes their potential applications in regenerative medicine, and lists their limitations, such as donor’s unknown medical condition in criminal cases, limited differentiation potential, higher risk of carcinogenesis, or changing DNA quality. Finally, the review underlines the need to develop procedures determining the safe CaSCs harvesting and use.

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Section: Limitations and Possibilitiesmentioning

confidence: 99%

Cadaveric Stem Cells: Their Research Potential and Limitations

Cieśla

Tomsia

2021

Front. Genet.

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“…There are a variety of cellular labels, although few have strong clinical potential. Magnetic and radioactive labels are the most prevalent due to their very high sensitivity and detection by magnetic resonance imaging (MRI) and positron emission tomography (PET), both clinically applicable imaging modalities [5,6]. While studies on the stability of radioactive labeling of stem cells are scarce, there are a lot of confusing data with regard to magnetic labeling of stem cells, which is worth further investigation.…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stem Cells Do Not Lose Direct Labels Including Iron Oxide Nanoparticles and DFO-89Zr Chelates through Secretion of Extracellular Vesicles

et al. 2021

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Rapidly ageing populations are beset by tissue wear and damage. Stem cell-based regenerative medicine is considered a solution. Years of research point to two important aspects: (1) the use of cellular imaging to achieve sufficient precision of therapeutic intervention, and the fact that (2) many therapeutic actions are executed through extracellular vesicles (EV), released by stem cells. Therefore, there is an urgent need to interrogate cellular labels in the context of EV release. We studied clinically applicable cellular labels: superparamagnetic iron oxide nanoparticles (SPION), and radionuclide detectable by two main imaging modalities: MRI and PET. We have demonstrated effective stem cell labeling using both labels. Then, we obtained EVs from cell cultures and tested for the presence of cellular labels. We did not find either magnetic or radioactive labels in EVs. Therefore, we report that stem cells do not lose labels in released EVs, which indicates the reliability of stem cell magnetic and radioactive labeling, and that there is no interference of labels with EV content. In conclusion, we observed that direct cellular labeling seems to be an attractive approach to monitoring stem cell delivery, and that, importantly, labels neither locate in EVs nor affect their basic properties.

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“…There are various approaches to pre- and postmortem imaging of transplanted cells with a variable robustness and complexity [11]. The simplest approach is based on pre-transplantation labeling of stem cells with a fluorescent tag followed by a postmortem identification using microscopy.…”

Section: Introductionmentioning

confidence: 99%

Labeling of human mesenchymal stem cells with different classes of vital stains: robustness and toxicity

et al. 2019

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Background Mesenchymal stem cell (MSC) transplantation has been explored as a new clinical approach to repair injured tissues. However, in order to evaluate the therapeutic activity of MSC, cell tracking techniques are required to determine the fate of transplanted cells in both preclinical and clinical studies. In these aspects, different vital stains offer the potential for labeling and monitoring of grafted cells in vivo . It is desirable to have tracking agents which have long-term stability, are not toxic to the cells, and do not affect cell function. Methods Here, we selected three different labels: CellTracker™ Green CMFDA, eGFP-mRNA (genetic pre-tag), and Molday ION Rhodamine B™ (nanoparticle-based fluorescent and magnetic label) and performed extensive analysis of their influence on in vitro expansion of human bone marrow-derived mesenchymal stem cells (hBM-MSCs), as well as potential of affecting therapeutic activity and the impact on the durability of staining. Results Our study showed that basic hBM-MSC characteristics and functions might be affected by labeling. We observed strong alterations of metabolic activity and morphology after eGFP and CellTracker™ Green CMFDA hBM-MSC staining. Molday ION Rhodamine B™ labeling revealed superior properties relatively to other vital stains. The relative expression level of most of the investigated growth factors remained stable after cell labeling, but we have observed some changes in the case of EGF, GDNF, HGF, and IGF gene expression. Conclusions Taken together, we suggest performing similar to ours extensive analysis prior to using any cell label to tag MSC in experiments, as it can thoroughly bias results. Electronic supplementary material The online version of this article (10.1186/s13287-019-1296-8) contains supplementary material, which is available to authorized users.

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Pre- and postmortem imaging of transplanted cells

Cited by 7 publications

References 174 publications

Cadaveric Stem Cells: Their Research Potential and Limitations

Cadaveric Stem Cells: Their Research Potential and Limitations

Mesenchymal Stem Cells Do Not Lose Direct Labels Including Iron Oxide Nanoparticles and DFO-89Zr Chelates through Secretion of Extracellular Vesicles

Labeling of human mesenchymal stem cells with different classes of vital stains: robustness and toxicity

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