PRE-CLINICAL BNCT GLIOBLASTOMA RESEARCH. ICP-AES BORON DETERMINATION METHOD. STUDIES ON ¹⁰ B BORON BIODISTRIBUTION IN MICE’S ORGANS

Abstract

To accompany boron neutron capture therapy for cancerous tumours, there has been an optimised ICP-AES method for boron determination in animals' organs tissues. This technique is characterised by its universalism. The approach comprises preliminary acid decomposition at high temperatures and pressure (if necessary), ICP-AES boron determination in the gained solutions analysing comparative samples basing on a single element solution. Its validity is proved by the spike experiment and mass sample variation test. The ICP-AES method is used during the evaluation of BPA and BSH accumulation in organs and tissues when intravenously injecting U87 glioblastoma medication to SCID mice of SPF-status.