Pre-Eclampsia: Microbiota possibly playing a role

Ahmadian, Elham; Saadat, Yalda Rahbar; Khatibi, Seyed Mahdi Hosseiniyan; Nariman‐Saleh‐Fam, Ziba; Bastami, Milad; Vahed, Fatemeh Zununi; Ardalan, Mohammadreza; Vahed, Sepideh Zununi

doi:10.1016/j.phrs.2020.104692

Cited by 33 publications

(17 citation statements)

References 92 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subsequently, GO enrichment of DERNAs suggested that immune injury substantially linked endothelial dysfunction to PE and thus might affect intercellular signaling; KEGG enrichment revealed that the microbiome and metabolic disorders had a profound influence on endothelial dysfunction and PE. These findings were consistent with those of previous studies on the mechanism of endothelial dysfunction in PE (Ahmadian et al, 2020;Aneman et al, 2020;Aplin et al, 2020;Vicen et al, 2020). In addition, it was remarkable that several reported PE-associated pathways, including the "Wnt signaling pathway" (Schlosser et al, 2020), "Toll-like receptor signaling pathway" (He et al, 2018), "mTOR signaling pathway" (He et al, 2019), "p53 signaling pathway" (Gao et al, 2016), and "JAK-STAT signaling pathway" (Zhao and Jiang, 2018) were also depicted.…”

Section: Discussionsupporting

confidence: 90%

Identification of mRNA-, circRNA- and lncRNA- Associated ceRNA Networks and Potential Biomarkers for Preeclampsia From Umbilical Vein Endothelial Cells

Chen

Zheng

et al. 2021

Front. Mol. Biosci.

View full text Add to dashboard Cite

ObjectiveThe etiology and pathogenesis of preeclampsia (PE) remain unclear, and ideal biomarkers for the early detection of PE are scarce. The involvement of the competing endogenous RNA (ceRNA) hypothesis in PE is only partially understood. The present study aimed to delineate a regulatory network in PE comprised of messenger RNAs (mRNAs), circular RNAs (circRNAs), long non-coding RNAs (lncRNAs), and microRNAs (miRNAs) via ceRNA profiles from human umbilical vein endothelial cells (HUVECs) to further reveal the pathogenesis of PE and potential biomarkers.MethodsDifferentially expressed mRNAs, circRNAs, and lncRNAs were detected in HUVECs from early onset preeclampsia (EOPE) cases (n = 4) and normal pregnancies (n = 4) by microarray analysis. Bioinformatics analysis was performed to systematically analyze the data, and a relevant ceRNA network was constructed. RNAs (ANGPT2, LIPG, hsa_circ_0025992, hsa_circ_0090396, hsa_circ_0066955, hsa_circ_0041203, hsa_circ_0018116, lnc-C17orf64-1:1, lnc-SLC27A2-2:1, and lnc-UEVLD-5:1) were validated by quantitative real-time PCR (qRT-PCR) in 10 pairs of HUVECs and placental tissues from PE patients and normal pregnancies. Furthermore, expression of hsa_circ_0025992 was detected in maternal peripheral blood samples from PE patients (n = 24) and normal pregnancies (n = 30) to confirm its potential as a novel biomarker. The receiver operating characteristic (ROC) curve was applied to analyze its diagnostic value.ResultsCompared with HUVECs from normal pregnancies, HUVECs from EOPE cases had 33 differentially expressed mRNAs (DEmRNAs), 272 DEcircRNAs, and 207 DElncRNAs. GO and KEGG analyses of the DERNAs revealed the biological processes and pathways involved in PE. Based on the microarray data and the predicted miRNAs, a ceRNA network was constructed with four mRNAs, 34 circRNAs, nine lncRNAs, and 99 miRNAs. GO and KEGG analyses of the network reinforced the crucial roles of metabolic disorders, the p53 and JAK/STAT signaling pathways in PE. In addition, ROC analysis indicated that hsa_circ_0025992 could be used as a novel biomarker for PE.ConclusionA novel ceRNA network was revealed in PE, and the potential of hsa_circ_0025992 to serve as a new biomarker was confirmed.

show abstract

Section: Discussionsupporting

confidence: 90%

Identification of mRNA-, circRNA- and lncRNA- Associated ceRNA Networks and Potential Biomarkers for Preeclampsia From Umbilical Vein Endothelial Cells

Chen

Zheng

et al. 2021

Front. Mol. Biosci.

View full text Add to dashboard Cite

show abstract

“…In this study, the decreased Phocaeicola (former name: Bacteroides ) vulgatus was worth noting, which was not only involved in the folate biosynthesis, etc., but also could fight against LPS-induced acute intestinal injury and DSS-induced colitis ( Li et al., 2021 ; Wang et al., 2022 ). After all, these are the potential risk factors of PE ( Ahmadian et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Preeclampsia (PE) is a complex multi-system disease with maternal hypertension and systemic arteriole spasm as basic pathological changes, leading to the second cause of maternal death worldwide ( Brown et al., 2018 ). In addition to severe complications such as eclampsia, cerebral hemorrhage and multiple organ failure, it can also cause fetal intrauterine growth restriction (FGR), fetal distress, premature delivery, stillbirth, and so on ( Ahmadian et al., 2020 ). It is an important factor in increasing perinatal morbidity and mortality, and a serious burden on patients and families.…”

Section: Introductionmentioning

confidence: 99%

Deep metagenomic characterization of gut microbial community and function in preeclampsia

Li²,

Wen

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Preeclampsia (PE) is a pregnancy complication characterized by severe hypertension and multiple organ damage. Gut microbiota has been linked to PE by previous amplicon sequencing studies. To resolve the PE gut microbiota in a higher taxonomy resolution, we performed shotgun metagenomic sequencing on the fecal samples from 40 early-onset PE and 37 healthy pregnant women. We recovered 1,750 metagenome-assembled genomes (representing 406 species) from the metagenomic dataset and profiled their abundances. We found that PE gut microbiota had enriched in some species belonging to Blautia, Pauljensenia, Ruminococcus, and Collinsella and microbial functions such as the bacitracin/lantibiotics transport system, maltooligosaccharide transport system, multidrug efflux pump, and rhamnose transport system. Conversely, the gut microbiome of healthy pregnant women was enriched in species of Bacteroides and Phocaeicola and microbial functions including the porphyrin and chlorophyll metabolism, pyridoxal-P biosynthesis, riboflavin metabolism, and folate biosynthesis pathway. PE diagnostic potential of gut microbial biomarkers was developed using both species and function profile data. These results will help to explore the relationships between gut bacteria and PE and provide new insights into PE early warning.

show abstract

“…24) These data suggest that changes in the maternal microbiome may play a role in the development of pre-eclampsia and provide insight into mechanisms involved in the onset of this disorder. 25,26) Associations between the vaginal microbiome and specific bacteria and preterm births have been reported. 27) Although ample evidence exists that gut microbiota directly or indirectly plays a role in the pathogenesis of hypertension and pre-eclampsia, less information is available on the vaginal microbiome of women with pre-eclampsia.…”

Section: Discussionmentioning

confidence: 99%

High serum concentrations of lipopolysaccharide binding protein in pregnancies with pre-eclampsia

Sakabe

Nishizawa

Kato

et al. 2021

Hypertens Res Pregnancy

View full text Add to dashboard Cite

Pre-eclampsia is considered a multi-factorial disease, in which both fetal and maternal factors are involved in its onset. In this study, we aimed to test the longstanding hypothesis that the immune response to bacterial endotoxin could explain the etiology of pre-eclampsia. Methods: Serum lipopolysaccharide binding protein (LBP) concentrations and the vaginal microbiome were compared between subjects with pre-eclampsia and those with normotensive pregnancies. Results: Serum LBP concentrations were higher in subjects with pre-eclampsia compared to those with normotensive pregnancies. LBP concentrations were poorly correlated with disease onset, blood pressure, birthweight, and placental weight. Moreover, there was no evidence of bacterial species specific to pre-eclampsia based on a comparative analysis of the vaginal microbiome of subjects with pre-eclampsia and those with normotensive pregnancies. Conclusion: Serum LBP concentrations were significantly higher in subjects with pre-eclampsia compared to those with normotensive pregnancies. The clinical significance of high serum LBP concentrations in pre-eclampsia requires further investigation.

show abstract

Pre-Eclampsia: Microbiota possibly playing a role

Cited by 33 publications

References 92 publications

Identification of mRNA-, circRNA- and lncRNA- Associated ceRNA Networks and Potential Biomarkers for Preeclampsia From Umbilical Vein Endothelial Cells

Identification of mRNA-, circRNA- and lncRNA- Associated ceRNA Networks and Potential Biomarkers for Preeclampsia From Umbilical Vein Endothelial Cells

Deep metagenomic characterization of gut microbial community and function in preeclampsia

High serum concentrations of lipopolysaccharide binding protein in pregnancies with pre-eclampsia

Contact Info

Product

Resources

About