Pre-harvest biocontrol of Listeria and Escherichia coli O157 on lettuce and spinach by lactic acid bacteria

Yin, Hsin‐Bai; Chen, Chi-Hung; Gu, Ganyu; Nou, Xiangwu; Patel, Jitendra

doi:10.1016/j.ijfoodmicro.2022.110051

Cited by 7 publications

(4 citation statements)

References 37 publications

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“…The latest research investigated LAB's efficiency as a protective culture tool to control L. monocytogenes in ready-to-eat and dairy-ripened products [110]. Moreover, Yin et al confirmed the possible use of LAB as biocontrol agents for limiting/inhibiting pathogens contamination on leafy greens [32]. Here, we investigated the ability of Lpb.…”

Section: Discussionmentioning

confidence: 97%

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Lactiplantibacillus plantarum from Unexplored Tunisian Ecological Niches: Antimicrobial Potential, Probiotic and Food Applications

Selmi,

Rocchetti,

Capozzi

et al. 2023

Microorganisms

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The continued exploration of the diversity of lactic acid bacteria in little-studied ecological niches represents a fundamental activity to understand the diffusion and biotechnological significance of this heterogeneous class of prokaryotes. In this study, Lactiplantibacillus plantarum (Lpb. plantarum) strains were isolated from Tunisian vegetable sources, including fermented olive and fermented pepper, and from dead locust intestines, which were subsequently evaluated for their antimicrobial activity against foodborne pathogenic bacteria, including Escherichia coli O157:H7 CECT 4267 and Listeria monocytogenes CECT 4031, as well as against some fungi, including Penicillium expansum, Aspergilus niger, and Botrytis cinerea. In addition, their resistance to oro-gastro-intestinal transit, aggregation capabilities, biofilm production capacity, adhesion to human enterocyte-like cells, and cytotoxicity to colorectal adenocarcinoma cell line were determined. Further, adhesion to tomatoes and the biocontrol potential of this model food matrix were analyzed. It was found that all the strains were able to inhibit the indicator growth, mostly through organic acid production. Furthermore, these strains showed promising probiotic traits, including in vitro tolerance to oro-gastrointestinal conditions, and adhesion to abiotic surfaces and Caco-2 cells. Moreover, all tested Lpb. plantarum strains were able to adhere to tomatoes with similar rates (4.0–6.0 LogCFU/g tomato). The co-culture of LAB strains with pathogens on tomatoes showed that Lpb. plantarum could be a good candidate to control pathogen growth. Nonetheless, further studies are needed to guarantee their use as probiotic strains for biocontrol on food matrices.

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Section: Discussionmentioning

confidence: 97%

“…In this regard, Pediococcus pentosaceus [31], Lbp. plantarum, Lactococcus lactis [32], and Latilactobacillus graminis [33] have been described as promising species for preventing biological contamination and spoilage of fresh vegetables.…”

Section: Introductionmentioning

confidence: 99%

Lactiplantibacillus plantarum from Unexplored Tunisian Ecological Niches: Antimicrobial Potential, Probiotic and Food Applications

Selmi,

Rocchetti,

Capozzi

et al. 2023

Microorganisms

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“…When CE treatment was applied to fresh produce and packaged foods, the efficacy of CE treatment was impacted by the vegetable type and packaging materials. No noticeable antagonistic action against E. coli O157: H7 and L. monocytogenes induced by Lactobacillus was found in fresh-cut cabbages [106], but 1-2 logs of these two pathogens were found to be reduced on lettuce and spinach in a field study [107]. These observations were probably due to the catalase activity in the cut cabbages, which adversely affects the function of CE.…”

Section: Application Of Ce Strategies To Biologically Control Plant-o...mentioning

confidence: 88%

Perspectives on Using a Competitive Exclusion Approach to Control Listeria monocytogenes in Biological Soil Amendments of Animal Origin (BSAAO): A Review

Wang

Huang

Jiang

2023

Applied Microbiology

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Biological soil amendments of animal origin (BSAAO), such as animal waste or animal-waste-based composts, may contain foodborne pathogens such as Listeria monocytogenes. Due to the ubiquitous nature of Listeria, it is essential to understand the behavior of L. monocytogenes in BSAAO in order to develop preharvest prevention strategies to reduce pathogen contamination. As biological control agents, competitive exclusion (CE) microorganisms have been widely utilized in agriculture to control plant- or foodborne pathogens. Due to the diverse microbial community, animal wastes and composts are the potential sources for isolating CE strains for pathogen control. To explore the potential of using CE to control L. monocytogenes in BSAAO, we thoroughly reviewed the studies on the fate of L. monocytogenes in the agriculture field, and in the isolation and identification of CE from different matrices, and the applications of CE as a biological control method. Future studies using a next-generation sequencing approach to identify and characterize CE strains in complex microbial communities can provide a comprehensive picture of the microbial interactions between invading pathogens and the indigenous microbiota in BSAAO. This comprehensive review will provide insight into the development of effective biological control measures for preventing L. monocytogenes contamination in the agricultural field and enhancing food safety.

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“…Also, in spinach and lettuce leaves, the spraying of a LAB cocktail ( L. lactis , Lactiplantibacillus plantarum , Lactobacillus johnsonii , and L. acidophilus ) reduced in 1–2 log 10 the viable cells of L. monocytogenes and E. coli O157:H7, under growth chamber conditions. Surprisingly the antimicrobial effect of LAB against E. coli O157:H7 remained effective for up to 5 days after inoculation (Yin et al, 2023).…”

Section: Biological Control Agents For Human Pathogens On Producementioning

confidence: 99%

Adhesion to and survival of foodborne pathogens in produce and strategies for their biocontrol

Barba‐León,

Arista‐Regalado,

Mancilla‐Becerra

et al. 2024

Journal of Food Safety

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Foodborne pathogens can cause gastrointestinal infections in consumers and in some cases can even lead to outbreaks. In the last decade, it has been observed that some zoonotic pathogenic bacteria can use plants as secondary hosts. Contamination with foodborne bacteria becomes relevant in foods that are regularly eaten raw, such as lettuce, cilantro, fenugreek, rocket leaves, basil, and so forth, and some fruits such as tomatoes, melons, and green peppers; because the elimination of these pathogenic bacteria is difficult to achieve with conventional sanitization processes. Contamination of produce can occur throughout the entire production chain. In farmlands, pathogenic bacteria can contaminate the seed, mainly when contaminated water is used for irrigation. Later, bacteria can reach other plant tissues such as the stems, leaves, and fruits. Another form of contamination is when the produce is in contact with feces from domestic, production, or wild animals. Additionally, poor handling practices during harvest, packaging, distribution, and sale can contaminate produce. Studies have shown that foodborne pathogens can adhere to produce, sometimes forming a biofilm, and can also be internalized into the plant or fruit, which protects them from sanitation processes. For this reason, in this text we address three biocontrol strategies such as bacteria, lytic bacteriophages, and some fungi, as an alternative approach for the control of both foodborne and plant pathogens. Additionally, the use of these biological agents can represent an advantage for the development of the plant, making them a good strategy to favor yield.

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Pre-harvest biocontrol of Listeria and Escherichia coli O157 on lettuce and spinach by lactic acid bacteria

Cited by 7 publications

References 37 publications

Lactiplantibacillus plantarum from Unexplored Tunisian Ecological Niches: Antimicrobial Potential, Probiotic and Food Applications

Lactiplantibacillus plantarum from Unexplored Tunisian Ecological Niches: Antimicrobial Potential, Probiotic and Food Applications

Perspectives on Using a Competitive Exclusion Approach to Control Listeria monocytogenes in Biological Soil Amendments of Animal Origin (BSAAO): A Review

Adhesion to and survival of foodborne pathogens in produce and strategies for their biocontrol

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