Mobility digital twins (MDTs), which utilize multi-modal microscopic (micro) traffic simulations and an activity-based demand generation, are envisioned as flexible and reliable planning tools for addressing today’s increasingly complex and diverse transport scenarios. Hybrid models may become a resource-efficient solution for building MDTs by creating large-scale, mesoscopic (meso) traffic simulations, using simplified, queue-based network-link models, in combination with more detailed local micro-traffic simulations focused on areas of interest. The overall objective of this paper is to develop an efficient toolchain capable of automatically generating, calibrating, and validating hybrid scenarios, with the following specific goals: (i) an automated and seamless merge of the meso- and micro-networks and demand; (ii) a validation procedure that incorporates real-world data into the hybrid model, enabling the meso- and micro-sub-models to be validated separately and compared to determine which simulation, micro- or meso-, more accurately reflects reality. The developed toolchain is implemented and applied to a case study of Munich, Germany, with the micro-simulation focusing on the city quarter of Schwabing, using real-word traffic flow and floating car data for validation. When validating the simulated flows with the detected flows, the regression curve shows acceptable values. The speed validation with floating car data reveals significant differences; however, it demonstrates that the micro-simulation achieves considerably better agreement with real speeds compared to the meso-model, as expected.