Pre-Transplant Myeloid Dendritic Cell Deficiency Associated with Cytomegalovirus Infection and Death after Kidney Transplantation

Sun, Qianmei; Hall, Eric R.; Huang, Yanfei; Chen, P.; Dibadj, K.; Murawski, Matthew R.; Shraybman, Renata; Ka, Kirk; Tang, Vitor; Peng, Ruihua; Patton, Pamela R.; Clare‐Salzler, Michael; Kaplan, Bruce; Segev, Dorry L.; Womer, Karl L.

doi:10.1097/00007890-201211271-00066

Cited by 1 publication

(6 citation statements)

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“…It is possible that the lower BDC levels in the HD unit patients reflect the more debilitated state of these patients, the majority of whom had multiple medical co‐morbidities and would never be considered for transplantation. However, in our previous studies, we have been unable to demonstrate an association of serum albumin, a marker for nutritional status, with either BDC level or post‐transplant viral infection . Likewise, in our combined work, we have never been able to demonstrate an association of time on HD with either of these outcomes.…”

Section: Discussioncontrasting

confidence: 62%

“…Our previous work demonstrated an association between pretransplant mDC deficiency and post‐transplant reactivation of BKV and CMV . Given the importance of DCs to maintenance of the lymphocyte pool and the critical role CD8 + T cells play in antiviral immunity, we determined the correlation of mDC and CD8 + T‐cell levels in the Eval/Tx and HD groups.…”

Section: Resultsmentioning

confidence: 99%

“…BDCs were analyzed by four‐color flow cytometry, as previously reported by our group and others , with limited modifications. The four‐color Dendritic Value Bundle Kit was obtained from BD Biosciences (San Jose, CA, USA), which includes fluorescein isothiocyanate (FITC)‐conjugated antilineage 1 (Lin1) cocktail antibodies, anti‐HLA‐DR‐PerCP, anti‐CD11c‐APC, anti‐CD123‐PE, and isotype control mouse IgG2a‐APC and IgG1‐PE antibodies.…”

Section: Methodsmentioning

confidence: 99%

“…However, additional DC subtypes are now known, with newer methods available to detect and isolate these cells . However, as the current report builds on our previous publications , we elected to continue to use the older technique.…”

Section: Methodsmentioning

confidence: 99%

“…Given the importance of DCs to immunity and the variability of levels observed in the kidney disease population, we have chosen to explore the association of BDC levels with post‐transplant viral infections. In two previous reports, we demonstrated an association of pretransplant mDC level deficiency with post‐transplant BK viremia and CMV disease . In an effort to evaluate how pretransplant BDC deficiency might influence these outcomes mechanistically, we studied the correlation of BDC deficiency with T‐cell level and function in patients with kidney disease.…”

Section: Introductionmentioning

confidence: 99%

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Blood dendritic cell levels associated with impaired IL-12 production and T-cell deficiency in patients with kidney disease: implications for post-transplant viral infections

et al. 2014

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Summary Reduced pretransplant blood myeloid dendritic cell (mDC) levels are associated with post-transplant BK viremia and cytomegalovirus (CMV) disease after kidney transplantation. To elucidate potential mechanisms by which mDC levels might influence these outcomes, we studied the association of mDC levels with mDC IL- 12 production and T-cell level/function. Peripheral blood (PB) was studied in three groups: (i) end stage renal disease patients on hemodialysis (HD; n = 81); (ii) chronic kidney disease stage IV-V patients presenting for kidney transplant evaluation or the day of transplantation (Eval/Tx; n = 323); and (iii) healthy controls (HC; n = 22). Along with a statistically significant reduction in mDC levels, reduced CD8+ T-cell levels were also demonstrated in the kidney disease groups compared with HC. Reduced PB mDC and monocyte-derived DC (MoDC) IL-12 production was observed after in vitro LPS stimulation in the HD versus HC groups. Finally, ELISpot assays demonstrated less robust CD3+ INF-γ responses by MoDCs pulsed with CMV pp65 peptide from HD patients compared with HC. PB mDC level deficiency in patients with kidney disease is associated with deficient IL-12 production and T-cell level/function, which may explain the known correlation of CD8+ T-cell lymphopenia with deficient post-transplant antiviral responses.

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Section: Discussioncontrasting

confidence: 62%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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