Pre-treatment of soybean plants with calcium stimulates ROS responses and mitigates infection by Sclerotinia sclerotiorum

Arfaoui, Arbia; Hadrami, Abdelbasset El; Daayf, Fouad

doi:10.1016/j.plaphy.2017.11.014

Cited by 30 publications

(19 citation statements)

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“…Stem rot caused by the phytopathogenic fungus Sclerotinia sclerotiorum is a very detrimental disease in many economically important crops including soybean, rapeseed oil, and sunflower (Wu et al, 2013; Arfaoui et al, 2018; Sabaté et al, 2018). S. sclerotiorum can infect more than 400 plant species belonging to 75 families and lead to typical stem rot symptoms, such as soft watery lesions or areas of light brown discoloration on leaves, main stems and branches (Kamal et al, 2015; Zhang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Due to the unique life cycle pattern, it is difficult to control the disease caused by S. sclerotiorum . Although various disease management strategies such as crop rotation, fungicide treatments and the use of resistant varieties have been employed, none of these strategies can completely control the disease (Arfaoui et al, 2018). With the increasing concerns on the environmental pollution, food safety and chemical pesticide resistance, the use of beneficial microorganisms is considered as an environmentally friendly alternative way to combat crop disease.…”

Section: Introductionmentioning

confidence: 99%

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Antifungal, Plant Growth-Promoting, and Genomic Properties of an Endophytic Actinobacterium Streptomyces sp. NEAU-S7GS2

Liu

Yan

et al. 2019

Front. Microbiol.

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Diseases caused by Sclerotinia sclerotiorum have caused severe losses of many economically important crops worldwide. Due to the long-term persistence of sclerotia in soil and the production of air-borne ascospores, synthetic fungicides play limited roles in controlling the diseases. The application of antagonistic microorganisms can effectively reduce the number of sclerotia and eventually eradicate S. sclerotiorum from soil, and therefore considerable interest has been focused on biological control. Streptomyces sp. NEAU-S7GS2 was isolated from the root of Glycine max and its rhizosphere soil. It showed significant inhibitory activity against the mycelial growth of S. sclerotiorum (99.1%) and completely inhibited sclerotia germination. Compared to the control, in the pot experiment the application of NEAU-S7GS2 not only demonstrated excellent potential to control sclerotinia stem rot of soybean with 77 and 38% decrease in disease incidence and disease index, respectively, but could promote the growth of soybean. The light microscopy and scanning electron microscopy showed that co-culture of NEAU-S7GS2 with S. sclerotiorum on potato dextrose agar could lead to contorted and fragmented mycelia of S. sclerotiorum, which was associated with the secretion of hydrolytic glucanase and cellulase and the production of active secondary metabolites by NEAU-S7GS2. The plant growth promoting activity of NEAU-S7GS2 was related to the solubilization of inorganic phosphate, and production of 1-aminocyclopropane-1-carboxylate (ACC) deaminase and indole acetic acid (IAA). To further explore the plant growth promoting and antifungal mechanisms, the complete genome of strain NEAU-S7GS2 was sequenced. Several genes associated with ammonia assimilation, phosphate solubilization and IAA synthesis, together with genes encoding ACC deaminase, glucanase and α-amylase, were identified. AntiSMASH analysis led to the identification of four gene clusters responsible for the biosynthesis of siderophores including desferrioxamine B and enterobactin. Moreover, the biosynthetic gene clusters of lydicamycins, phenazines, and a glycosylated polyol macrolide showing 88% gene similarity to PM100117/PM100118 were identified. These results suggested that strain NEAU-S7GS2 may be a potential biocontrol agent and biofertilizer used in agriculture.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antifungal, Plant Growth-Promoting, and Genomic Properties of an Endophytic Actinobacterium Streptomyces sp. NEAU-S7GS2

Liu

Yan

et al. 2019

Front. Microbiol.

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“…The mitochondrial membrane potential, Ca 2+ concentration, and ROS level are closely related to cell metabolism. Changes in mitochondrial membrane potential modulate the activity of important enzymes in mitochondria, and the mitochondrial membrane potential, or ΔΨ, is a reflection of mitochondrial metabolic status ( Arfaoui et al, 2018 ). Additionally, Ca 2+ regulates cell metabolism together with ROS ( Zhou et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Fra-1 Inhibits Cell Growth and the Warburg Effect in Cervical Cancer Cells via STAT1 Regulation of the p53 Signaling Pathway

Manying

Liang

et al. 2020

Front. Cell Dev. Biol.

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The oncogenesis of cervical cancer is a multi-factor and multi-step process, and major risk factors include oncogene activation with tumor suppressor gene inactivation, viral factors, and immune factors. For example, the human papillomavirus (HPV) has been linked to the occurrence of cervical cancer. At present, the pathogenesis of cervical cancer remains unclear. Fra-1 (Fos-related antigen 1, also known as FOSL1) is a member of the Fos family and an important nuclear transcription factor that regulates normal cell growth, differentiation, and apoptosis. In the present study, we found that Fra-1 inhibited the proliferation of cervical cancer cells while also promoting apoptosis and affecting cell cycle distribution. Moreover, Fra-1 up-regulated STAT1 expression and modulated p53 signal pathway activity in cervical cancer cells. Overexpression of Fra-1 inhibited cell senescence by altering sirtuin 1 (SIRT1) expression in HeLa cells, and Fra-1 overexpression restored mitochondrial disorder and suppressed metabolic reprogramming in HeLa cells. Silencing of STAT1 impaired the inhibitory effect of Fra-1 on cervical cancer cell growth, while knock-down of STAT1 reversed the effect on cell senescence and mitochondrial dysfunction caused by Fra-1 in HeLa cells. Silencing of STAT1 also recovered metabolic reprogramming in cervical cancer cells. In summary, our results show that Fra-1 inhibited cervical cancer cell growth and the Warburg effect via STAT1-mediated regulation of the p53 signaling pathway.

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“…In the plant– Sclerotinia system, pathogen detection is based on the recognition of pathogen-associated molecular patterns (PAMPs) by pattern recognition receptors, such as RLP30 (receptor-like protein 30) [ 7 ] and other receptor-like kinases, or via cytoplasmic leucine rich proteins (LRRs) [ 55 ]. The subsequent signal transduction involves a MAPK (mitogen-activated protein kinase) cascade or MAPK-independent pathways [ 56 ] involving many signaling molecules, including reactive oxygen species (ROS) [ 57 ], nitric oxide [ 58 ], salicylic acid [ 59 ], jasmonic acid and ethylene [ 60 ]. These signals drive plant PAMP-triggered immunity through the production of nuclear proteins including transcription factors and protein kinases that activate ROS production, detoxification, oxidative protection, callose deposition, camalexin production and the production of other specialized metabolites [ 61 , 62 ].…”

Section: Discussionmentioning

confidence: 99%

Differential Alternative Splicing Genes and Isoform Regulation Networks of Rapeseed (Brassica napus L.) Infected with Sclerotinia sclerotiorum

et al. 2020

Genes

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Alternative splicing (AS) is a post-transcriptional level of gene expression regulation that increases transcriptome and proteome diversity. How the AS landscape of rapeseed (Brassica napus L.) changes in response to the fungal pathogen Sclerotinia sclerotiorum is unknown. Here, we analyzed 18 RNA-seq libraries of mock-inoculated and S. sclerotiorum-inoculated susceptible and tolerant B. napus plants. We found that infection increased AS, with intron retention being the main AS event. To determine the key genes functioning in the AS response, we performed a differential AS (DAS) analysis. We identified 79 DAS genes, including those encoding splicing factors, defense response proteins, crucial transcription factors and enzymes. We generated coexpression networks based on the splicing isoforms, rather than the genes, to explore the genes’ diverse functions. Using this weighted gene coexpression network analysis alongside a gene ontology enrichment analysis, we identified 11 modules putatively involved in the pathogen defense response. Within these regulatory modules, six DAS genes (ascorbate peroxidase 1, ser/arg-rich protein 34a, unknown function 1138, nitrilase 2, v-atpase f, and amino acid transporter 1) were considered to encode key isoforms involved in the defense response. This study provides insight into the post-transcriptional response of B. napus to S. sclerotiorum infection.

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Pre-treatment of soybean plants with calcium stimulates ROS responses and mitigates infection by Sclerotinia sclerotiorum

Cited by 30 publications

References 50 publications

Antifungal, Plant Growth-Promoting, and Genomic Properties of an Endophytic Actinobacterium Streptomyces sp. NEAU-S7GS2

Antifungal, Plant Growth-Promoting, and Genomic Properties of an Endophytic Actinobacterium Streptomyces sp. NEAU-S7GS2

Fra-1 Inhibits Cell Growth and the Warburg Effect in Cervical Cancer Cells via STAT1 Regulation of the p53 Signaling Pathway

Differential Alternative Splicing Genes and Isoform Regulation Networks of Rapeseed (Brassica napus L.) Infected with Sclerotinia sclerotiorum

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