Pre-treatment with high molecular weight free PEG effectively suppresses anti-PEG antibody induction by PEG-liposomes in mice

McSweeney, Morgan D.; Shen, Limei; DeWalle, Alexander C.; Joiner, Jordan B.; Ciociola, Elizabeth C.; Raghuwanshi, Dharmendra; Macauley, Matthew; Lai, Samuel K.

doi:10.1016/j.jconrel.2020.10.011

Cited by 29 publications

(17 citation statements)

References 44 publications

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“…Even though PEG is nontoxic and nonimmunogenic, − the cytotoxicity of PEG (P8k) was evaluated by a standard MTT assay (see Supporting Information). , The results showed that, after culturing for 24 h in the presence of PEG (1–20 mg/mL), HeLa cell viability was fully kept at 100% (Figure S19), confirming that PEG is effectively non-cytotoxic and may be used for cellular imaging.…”

Section: Resultsmentioning

confidence: 99%

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Fluorescence Behavior and Mechanisms of Poly(ethylene glycol) and Their Applications in Fe³⁺ and Cr⁶⁺ Detections, Data Encryption, and Cell Imaging

Sun

Jiang

et al. 2021

ACS Sustainable Chem. Eng.

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In contrast to conventional fluorescent polymers featured by large conjugation structures, a new class of fluorescent polymers without any conjugations is gaining great interest in immerging applications beyond the possibility to achieve by the conjugated polymers. Poly(ethylene glycol) (PEG), widely used in biomedical fields for a long time owing to its nontoxicity and nonimmunogenicity, is found to be fluorescence emissive in the solid state and in aqueous solution, though deemed as not fluorescent in numerous reports. Through systematic study under different conditions, the emission is ascribed to the cluster formation of its chains; thereby the blue-shift of the emission with the excitation wavelength was interpreted through the Forster resonance energy transfer. The clusterization was ascertained through size measurements, Fourier transform infrared spectroscopy, NMR analyses, and the dependence on temperature, pH, and nonsolvent presence. Tested in the presence of competitive metal ions, selective emission quenching by Fe 3+ and Cr 6+ was observed. PEG was used as a sensor for the detection of Cr 6+ , Fe 3+ , and H 2 O 2 , outperforming most of the reported sensors alike. Its uses for data encryption and cell imaging were also presented. This work provides therefore a novel face of PEG with great potential in a variety of emerging applications, in particular, as sensors in the biomedical area.

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Section: Resultsmentioning

confidence: 99%

“…Poly(ethylene glycol) (PEG) has been well-known to be nontoxic and nonimmunogenic, − and approved by FDA for various uses . PEGs themselves and their derivatives are used extensively in biosciences, cosmetics, and in the pharmaceutical, food, and textile industries.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence Behavior and Mechanisms of Poly(ethylene glycol) and Their Applications in Fe³⁺ and Cr⁶⁺ Detections, Data Encryption, and Cell Imaging

Sun

Jiang

et al. 2021

ACS Sustainable Chem. Eng.

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“…These findings are contrary to the so-called "stealth effect" where PEGylation was thought to impart improved NP pharmacokinetic properties leading researchers to develop PEG alternatives and anti-PEG antibody accommodations. 15,16 Given this, there is a need to characterize the BC influence in both pristine and sterically-coated NP formulations. In addition to NP physicochemical properties, several parameters have a significant influence on the composition of the BC, including biomolecule binding affinities, relative concentration, and exposure time.…”

Section: Personalized Biomolecular Coronamentioning

confidence: 99%

Nanoparticle personalized biomolecular corona: implications of pre-existing conditions for immunomodulation and cancer

Shaw

Pearson

2022

Biomater. Sci.

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“…However, substantially improved circulation times are attained by adjusting the lipid composition of the membrane in order to minimize the undesired interactions with the defense system of the host. Addition of PEGylated lipids to the self-assembled membranes is often employed to extend the lifetime of liposomes in circulation, and such compositions are used for producing liposomes intended for cancer therapy and other in vivo applications [16][17][18]46,48,49]. To verify if electrodialysis is suitable for formation and loading of long-circulating liposomes, we prepared lipid mixtures containing DSPC, Chol, and DSPE-PEG (8.2:3.8:2.6 weight ratios).…”

Section: Production Of Long-circulating Liposomes By Electrodialysismentioning

confidence: 99%

Rapid Production and Purification of Dye-Loaded Liposomes by Electrodialysis-Driven Depletion

et al. 2021

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Liposomes are spherical-shaped vesicles that enclose an aqueous milieu surrounded by bilayer or multilayer membranes formed by self-assembly of lipid molecules. They are intensively exploited as either model membranes for fundamental studies or as vehicles for delivery of active substances in vivo and in vitro. Irrespective of the method adopted for production of loaded liposomes, obtaining the final purified product is often achieved by employing multiple, time consuming steps. To alleviate this problem, we propose a simplified approach for concomitant production and purification of loaded liposomes by exploiting the Electrodialysis-Driven Depletion of charged molecules from solutions. Our investigations show that electrically-driven migration of charged detergent and dye molecules from solutions that include natural or synthetic lipid mixtures leads to rapid self-assembly of loaded, purified liposomes, as inferred from microscopy and fluorescence spectroscopy assessments. In addition, the same procedure was successfully applied for incorporating PEGylated lipids into the membranes for the purpose of enabling long-circulation times needed for potential in vivo applications. Dynamic Light Scattering analyses and comparison of electrically-formed liposomes with liposomes produced by sonication or extrusion suggest potential use for numerous in vitro and in vivo applications.

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Pre-treatment with high molecular weight free PEG effectively suppresses anti-PEG antibody induction by PEG-liposomes in mice

Cited by 29 publications

References 44 publications

Fluorescence Behavior and Mechanisms of Poly(ethylene glycol) and Their Applications in Fe³⁺ and Cr⁶⁺ Detections, Data Encryption, and Cell Imaging

Fluorescence Behavior and Mechanisms of Poly(ethylene glycol) and Their Applications in Fe³⁺ and Cr⁶⁺ Detections, Data Encryption, and Cell Imaging

Nanoparticle personalized biomolecular corona: implications of pre-existing conditions for immunomodulation and cancer

Rapid Production and Purification of Dye-Loaded Liposomes by Electrodialysis-Driven Depletion

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Pre-treatment with high molecular weight free PEG effectively suppresses anti-PEG antibody induction by PEG-liposomes in mice

Cited by 29 publications

References 44 publications

Fluorescence Behavior and Mechanisms of Poly(ethylene glycol) and Their Applications in Fe3+ and Cr6+ Detections, Data Encryption, and Cell Imaging

Fluorescence Behavior and Mechanisms of Poly(ethylene glycol) and Their Applications in Fe3+ and Cr6+ Detections, Data Encryption, and Cell Imaging

Nanoparticle personalized biomolecular corona: implications of pre-existing conditions for immunomodulation and cancer

Rapid Production and Purification of Dye-Loaded Liposomes by Electrodialysis-Driven Depletion

Contact Info

Product

Resources

About

Fluorescence Behavior and Mechanisms of Poly(ethylene glycol) and Their Applications in Fe³⁺ and Cr⁶⁺ Detections, Data Encryption, and Cell Imaging

Fluorescence Behavior and Mechanisms of Poly(ethylene glycol) and Their Applications in Fe³⁺ and Cr⁶⁺ Detections, Data Encryption, and Cell Imaging