Objective: to verify measures used in intensive care units to prevent infection related to health care. Methods: integrative review based on the data sources LILACS, SCOPUS, PubMed Central, Science Direct, Web of Science and CINAHL, whose descriptors used were: Infection Control and Intensive Care Units. Sample consisting of 146 studies. Results: thematic categories related to the relevant infection prevention measures were listed, developed in Intensive Care Units: bloodstream infection prevention measures, assistance-related pneumonia prevention measures, urinary tract infection prevention measure and preventive measures associated with general care. Conclusion: among the established thematic categories, care with skin preparation, oral hygiene, correct handling of the urinary catheter and daily bath with antiseptic were most frequently identified.