Preadministration of yerba mate (Ilex paraguariensis) helps functional activity and morphology maintenance of MC3T3-E1 osteoblastic cells after in vitro exposition to hydrogen peroxide

Increased Osteoblastic and Osteocytic in Vitro Cell Viability by Yerba Mate (Ilex paraguariensis)

“…[ 36 ] It was also recently demonstrated that pre-administration of YM extract may prevent deleterious effects in cell morphology, increasing cell adhesion and proliferation rate in MC3T3-E1 cells exposed to H 2 O 2 , which could enable the maintenance of extracellular matrix in the presence of oxidative stress. [ 25 ] Moreover, a positive effect of low concentration of soluble YM on osteoblast of bone marrow-derived mesenchymal stromal cells differentiation was found, with increased alkaline phosphatase activity, mineralization and gene expression of transcription factors (Runx2, Osterix, and β-catenin) and bone matrix proteins (osteopontin, bone sialoprotein, osteocalcin, and bone morphogenetic protein-2). [ 26 ] However, the same study showed that a higher YM concentration (≥50 μg/mL) had deleterious effects, including cytotoxicity.…”

“…[ 23 ] Additionally, it has been suggested that foods rich in antioxidants may represent a strategy to decrease age-related bone loss, while foods rich in polyphenols have been associated with better bone health attributable to their antioxidant capacity. [ 20 , 24 ] However, although there are few in vitro studies reporting the effects of YM on osteoblastic cells,[ 25 , 26 ] whether particular components of the infusion have effects on cell survival or not remains unknown.…”

Ilex paraguariensis Extracts Prevent Oxidative Damage in a Mouse Model of Age‐Related Macular Degeneration

“…Therefore, when mice received YM before exposure to sodium iodate, products of NRF2 and some of its target genes, such as SOD2, were available before oxidative stress. Priming effects could be essential for protection, as has been suggested for osteoblastic maintenance, [38] cardiometabolic disease, [29] and hepatic damage in overweight rats. [39] By contrast, NRF2 upregulation in the presence of cell damage might potentiate antisurvival effects of P53.…”

Avaliação in vitro da pré-administração de licopeno na atividade funcional e na manutenção da morfologia das células osteoblásticas MC3T3-E1 cultivadas sobre discos de titânio nanotexturizado e submetidas ao estresse oxidativo

“…As concentrações que estressam as células em estudos in vitro e podem levar até à apoptose variam entre 300 µM e 500 µM (Dan et al, 2018). Foi seguido o protocolo de CEVERINO et al (2021), adicionando-se 400 μmol/L H2O2 à cultura e inicialmente partiu-se de uma solução de peróxido de hidrogênio a 30% (100 volumes). Nos primeiros experimentos, as células morreram sem a possibilidade de realizar qualquer ensaio.…”

“…A simulação do estresse oxidativo que acontece na osteoporose pode ser feita in vitro por meio da adição de peróxido de hidrogênio à cultura. Estudos anteriores realizados por nosso grupo de pesquisa em modelo in vitro indicam que a pré-administração de substâncias antioxidantes como o extrato de ervamate podem prevenir os efeitos deletérios da exposição celular ao peróxido de hidrogênio, simulando o estresse oxidativo (CEVERINO et al, 2021). Sendo assim, a hipótese este trabalho é a de que a administração do licopeno em meio de cultura com células osteoblásticas (MC3T3-E1) cultivadas sobre discos de titânio nanotexturizados e submetidas ao estresse oxidativo possa promover: 1) a atividade funcional celular e 2) a manutenção da morfologia celular.…”