There has been an increase in research concerning the quality of dyadic interactions between humans and dogs in university-based animal assisted activities (AAAs). While interactions between students and dogs are commonly an area of focus, studies examining interactions between handlers and their dogs are needed. We coded 10-min long video-recorded observations (N = 151) using a mixed methods approach to capture the duration and frequency of dog-directed handler behavior (i.e., postural state, verbal and physical contact) before and during interactions with student participants in an AAA. Analyses showed a significant decrease in the proportion of time handlers spent petting their dog, and a significant increase in the proportion of time handlers spent sitting on the floor and restraining their dog by the leash in response to student introduction. Two dimensions of handlers’ dog-directed behavior emerged (e.g., warmth, control) revealing distinct handler interaction styles: Hands-off (L Warmth, L Control), permissive (H warmth, L control), authoritative (H Warmth, H Control), and authoritarian (L Warmth, H Control). Handlers’ interaction styles were influenced by student presence, leading some handlers to increase warmth behaviors directed to their dogs, while others decreased such behaviors. Implications for the facilitation of university-based AAAs are discussed.