Preadsorption of polymers on glass and silica to reduce fibrinogen adsorption

Abstract

Glass and silica beads were precoated with various polymers to obtain steric exclusion chromatography (SEC) supports which are nonadsorbant for hydrophilic macromolecules. The efficiency of this treatment was estimated by subsequent radiolabeled fibrinogen adsorption. The result obtained with a block copolymer was better than with various hydrophilic homopolymers. This ABA type block copolymer, where A is a poly(N-acetylethyleneimine) (PAEI) sequence and B a polyethylene oxide (PEO) sequence was preadsorbed at pH 4.5 and 25 degrees C; the fibrinogen adsorption was reduced to less than 5% of the value observed on untreated solid surfaces. Thus the hemocompatibility of solid supports should be increased by precoating with this block copolymer. Results for nonporous glass beads and porous silica particles were in good correlation.

Deposition of 2‐oxazoline‐based plasma polymer coatings using atmospheric pressure helium plasma jet

“…In recent years, the interest in POx has grown substantially, largely due to the discovery that their excellent biocompatibility and "stealth" behaviour are comparable to the gold standard polyethylene glycol. [17][18][19][20][21] For many applications, especially in the biomedical field, POx are applied onto biomaterials surface as thin film coatings from aqueous solutions. [21][22][23][24] The current strategy for application of POx coatings on biomaterials is a multistep process, substrate-dependent, requires presynthesis of POx polymers and employs wet chemistry methods.…”

Reduced protein adsorption on plastics via direct plasma deposition of triethylene glycol monoallyl ether

“…A recent monoof PEO units to various substrates. Attachment procegraph on poly(ethylene glycol) chemistry provides dedures have ranged from simple physical adsorption tailed reviews of and abundant references for the effistudies [2][3][4][5][6][7][8] to structures achieved via covalent bonding cacy of surface-attached PEO molecules in reducing of PEO molecules to targeted surfaces. [9][10][11][12][13][14] These approtein and cellular adsorption on surfaces.…”

Easily grafted polyurethanes with reactive main chain functional groups. Synthesis, characterization, and antithrombogenicity of poly(ethylene glycol)-grafted poly(urethanes)

“…The aqueous portions were combined, neutralized with saturated aqueous citric acid, and extracted with diethyl ether (3 ϫ 50 mL). The ether extracts were dried over MgSO 4 …”

“…In the study of protein resistance by poly-(ethers), PEG has been introduced onto the surface of glass 3,4 and gold, 5 as well as incorporated into poly(ethylene terephthalate), 6 -9 poly(vinylidene chloride), 9 polypropylene, 9 poly(styrene), 10 and poly(urethanes). 11,12 Liposomes 13 and Langmuir-Blodgett phosphatidyl lipid bilayer films 14 incorporating even small amounts of PEG have shown to resist binding to blood proteins and cells.…”