Preanalytical Factors Affecting RIA Measurement of Plasma Kisspeptin

Ramachandran, Radha; Patterson, Michael; Murphy, Kevin G.; Dhillo, Waljit S.; Patel, Suhani; Казарян, А.В.; Ghatei, Mohammad A.; Bloom, Stephen R.

doi:10.1373/clinchem.2007.093005

Cited by 34 publications

(31 citation statements)

References 7 publications

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“…This was also true for each tumour subtype and indicates that a substantial and measurable systemic Kiss-10 expression even exists in patients with a low tumour burden as observed in this study. These findings are in line with the current literature in which elevated levels of Kisspeptin were found in tumour patients and low levels in controls were found most probably because of an inactivated kisspeptin system [26] .…”

Section: Discussionsupporting

confidence: 93%

Evaluation of Plasmatic Kisspetin-10 as a Biomarker for Malignancy and Subtype Differentiation in Small Renal Tumours

Horstmann

Krause²,

Steinbach³

et al. 2017

Urol Int

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Objective: To evaluate Kisspeptin-10 (Kiss-10) in patients with small renal tumours (SRTs) and controls. Material and Methods: Kiss-10 was measured in preoperative plasma samples in a cohort of 143 patients with unilateral renal tumours smaller than or equal to 4 cm and 40 age-matched controls by a competitive ELISA test kit. The cohort of patients included 56 patients with clear cell renal cell carcinoma (ccRCC), 43 with papillary RCC (pRCC), 12 with chromophobe RCC (chRCC) and 32 with oncocytomas. Results: Kiss-10 was detected in all patients and controls. SRT patients revealed significantly higher Kiss-10 levels than controls (mean value 10.04 vs. 6.37 pmol/l, p < 0.001). In SRT patients, Kiss-10 was detected at significantly different concentrations between the subgroups (p = 0.021). The highest concentration was observed in those with oncocytomas (11.50 pmol/) followed by chRCC, pRCC and ccRCC patients (9.89, 10.01 and 9.25 pmol/l, respectively). Receiver operating characteristic curve analyses revealed an area under the curve (AUC) of 0.82 for the comparison of all tumours vs. controls (p < 0.001) and an AUC of 0.671 for all malignant tumours vs. oncoytomas (p = 0.003). Conclusion: This study shows that Kiss-10 levels are significantly altered by malignancy and tumour subtypes even in patients with SRTs. Kiss-10 therefore deserves further attention as a plasmatic biomarker for renal tumours.

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Section: Discussionsupporting

confidence: 93%

Evaluation of Plasmatic Kisspetin-10 as a Biomarker for Malignancy and Subtype Differentiation in Small Renal Tumours

Horstmann

Krause²,

Steinbach³

et al. 2017

Urol Int

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“…Samples were centrifuged immediately (3000 rotation per minute), separated and stored at À20 C until measurement of kisspeptin immunoreactivity using a previously described, in-house radioimmunoassay based on a sheep GQ2 antibody. 6 Assay sensitivity was 2pmol/L, and the intra-and inter-assay coefficients of variation were 8.3% and 10.2%, respectively.…”

Section: Methodsmentioning

confidence: 93%

Age-dependent elevations in plasma kisspeptin are observed in boys and girls when compared with adults

et al. 2013

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Background: Kisspeptin is a hypothalamic neuropeptide playing a physiological role in human reproduction. Genetic over-activation of kisspeptin causes precocious puberty in children. Concentrations of circulating kisspeptin are low in adults. The concentrations of plasma kisspeptin in boys and girls have not been studied previously. Methods: Blood was obtained from 51 children and 63 adults. Plasma samples were analysed using radioimmunoassay. Children were aged 2-18 years, and attending hospital for a medically requested blood test unrelated to reproductive development. Data on pubertal status were not collected due to ethical reasons. Results: Mean plasma kisspeptin was significantly higher in children when compared with adults (mean plasma kisspeptin in pmol/L: 12.3 AE 0.9, adults; 40.9 AE 3.3, children, P < 0.001 vs. adults). Overall mean concentrations of plasma kisspeptin were not significantly different between sexes (mean plasma kisspeptin in pmol/L: 39.5 AE 3.2, boys; 44.3 AE 6.3, girls, P ¼ 0.48). In both sexes, concentrations of plasma kisspeptin increased with age to peak concentrations between 9 and 12 years of age, before decreasing beyond 12 years of age to adulthood. Plasma kisspeptin concentrations were highly significantly elevated in both girls and boys aged 9-12 when compared with adults (mean plasma kisspeptin in pmol/L: 59.5 AE 18.3, girls, P < 0.01 vs. adult women; 43.8 AE 6.2, boys, P < 0.001 vs. adult men). Conclusions: We report that circulating kisspeptin is elevated in both boys and girls when compared with adults. Furthermore both boys and girls may have distinct, age-dependent concentrations of circulating kisspeptin. Further studies may determine if plasma kisspeptin could be used as a clinically useful biochemical marker of reproductive development in children.

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“…Measurements using a commercially available assay suggested that the intravenous administration schedule used in our in vivo experiments produced increases in plasma kisspeptin (9 nmol/l) of the same order of magnitude as the physiological increases (2.5 nmol/l) reported in pregnant women [19], suggesting that there are circumstances where circulating kisspeptin could influence islet function. However, some caution is required in interpreting these data, since plasma kisspeptin can be difficult to measure accurately [35]. In the current study we used a human kisspeptin assay because there are no available assays for rat kisspeptin and the inter-species specificity of the assay is unknown.…”

Section: Discussionmentioning

confidence: 99%

Kisspeptin stimulation of insulin secretion: mechanisms of action in mouse islets and rats

et al. 2009

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Aims/hypothesis Kisspeptin is a novel peptide identified as an endogenous ligand of the G-protein-coupled receptor 54 (GPR-54), which plays a crucial role in puberty and reproductive function. High levels of GPR-54 and kisspeptin have been reported in the pancreas and we have previously shown that kisspeptin potentiates glucose-induced insulin release from isolated islets, although the mechanisms underlying this effect were unclear. Methods Insulin secretion from isolated mouse islets was measured to characterise the effects of kisspeptin. The effects of kisspeptin on both p42/44 mitogen-activated protein kinase (MAPK) phosphorylation and intracellular Ca 2+ ([Ca 2+ ] i ) in mouse islets were also investigated. Furthermore, kisspeptin was administered to rats in vivo and effects on plasma insulin levels measured. Results In the current study, kisspeptin induced a concentration-dependent potentiation of glucose-induced (20 mmol/l) insulin secretion from mouse islets, with maximal effects at 1 µmol/l, but had no effect on insulin secretion at a substimulatory concentration of glucose (2 mmol/l). Activation of GPR-54 by kisspeptin also caused reversible increases in [Ca 2+ ] i in Fura-2 loaded dispersed islet cells.The kisspeptin-induced potentiation of glucose-induced insulin secretion was completely abolished by inhibitors of phospholipase C and p42/44 MAPK, but not by inhibitors of protein kinase C or p38 MAPK. Intravenous administration of kisspeptin into conscious, unrestrained rats caused an increase in circulating insulin levels, whilst central administration of kisspeptin had no effect, indicating a peripheral site of action. Conclusions/interpretation These observations suggest that neither typical protein kinase C isoforms nor p38 MAPK are involved in the potentiation of glucose-induced insulin release by kisspeptin, but intracellular signalling pathways involving phospholipase C, p42/44 MAPK and increased [Ca 2+ ] i are required for the stimulatory effects on insulin secretion. The observation that kisspeptin is also capable of stimulating insulin release in vivo supports the conclusion that kisspeptin is a regulator of beta cell function.

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Preanalytical Factors Affecting RIA Measurement of Plasma Kisspeptin

Abstract: Kisspeptins are peptide products of the KiSS-1 metastasis-suppressor (KISS1) gene and the natural ligands of the G-protein-coupled receptor GPR54. KISS1 was initially investigated as an antimetastasis gene. More recent studies have demonstrated that the kisspeptins are potent stimula

Cited by 34 publications

References 7 publications

Evaluation of Plasmatic Kisspetin-10 as a Biomarker for Malignancy and Subtype Differentiation in Small Renal Tumours

Evaluation of Plasmatic Kisspetin-10 as a Biomarker for Malignancy and Subtype Differentiation in Small Renal Tumours

Age-dependent elevations in plasma kisspeptin are observed in boys and girls when compared with adults

Kisspeptin stimulation of insulin secretion: mechanisms of action in mouse islets and rats

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