Preanalytical Stability of Adrenocorticotropic Hormone Depends on Time to Centrifugation Rather than Temperature

Reisch, Nicole; Reincke, Martín; Bidlingmaier, Martin

doi:10.1373/clinchem.2006.080622

Cited by 31 publications

(19 citation statements)

References 5 publications

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“…8,9 Our results are consistent with recent studies using modern assays that suggest that ACTH is stable for at least 6 hours at room temperature in whole blood collected onto EDTA plasma with some studies suggesting longer stability (up to 24 hours). 6,7,10 They are also consistent with previous research suggesting greater post-centrifugation stability in EDTA plasma than serum gel. 11 Previous research into the stability of aldosterone is also consistent with our findings, with aldosterone previously demonstrated to be stable for over 13 hours in both EDTA plasma and serum gel.…”

Section: Discussionsupporting

confidence: 90%

Current laboratory requirements for adrenocorticotropic hormone and renin/aldosterone sample handling are unnecessarily restrictive

et al. 2017

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Samples for adrenocorticotropic hormone (ACTH) and aldosterone/renin analysis usually require rapid transport to the receiving laboratory for immediate separation and freezing. In practice, this means assessment is limited to hospital settings and many samples are rejected. We examined whether these requirements are necessary by assessing the stability of ACTH, aldosterone and renin over 48 hours in whole blood collected in serum gel and EDTA plasma from 31 participants. Our results show that ACTH collected into EDTA plasma is stable at room temperature for at least 6 hours, mean change at 6 hours -2.6% (95% CI -9.7 to 4.5). Both aldosterone and renin were stable collected on serum gel at room temperature for at least 6 hours: mean change aldosterone +0.2% (95% CI -3.6 to 4.0), renin -1.9% (95% CI -7.0 to3.2). Therefore, by using appropriate preservatives, ACTH and aldosterone/renin can be measured on samples collected at room temperature and processed within 6 hours. This would facilitate outpatient and emergency room assessment of these analytes.

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Section: Discussionsupporting

confidence: 90%

Current laboratory requirements for adrenocorticotropic hormone and renin/aldosterone sample handling are unnecessarily restrictive

et al. 2017

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“…These results are similar to those of investigations performed with human plasma, in which ACTH concentrations were demonstrated to remain within 10% of original values for up to 8 h at 30°C and 18-24 h at 24°C [12][13][14]. Although the effects of storage at lower temperatures were not investigated in the present study, in human plasma there was minimal protective benefit in reducing the storage temperature from 24 to 4°C [12,13]. However, the mechanism responsible for the inactivation of ACTH does appear to be influenced by higher temperatures, as rapid degradation above 30°C has been reported [15].…”

Section: Table 1: Median (Range) Adrenocorticotrophic Hormone (Acth) supporting

confidence: 90%

“…In addition to imprecision of the assay, other possible explanations would be cross-reactivity of the assay with other components Equine Veterinary Journal 47 (2015) 587-591 © 2014 EVJ Ltd of plasma, ACTH release from a binding protein or reservoir of hormone, or matrix changes that could influence interference [12]. Some of the variation seen over time may have been the result of analytical error; however, the manufacturers of the chemiluminescent device used in the present investigation claim <9% variance (Immulite Product Manual, Siemens), and the coefficient of variation for the assay in the test laboratory was 6%, so it is unlikely that assay variability influenced results significantly.…”

Section: Table 1: Median (Range) Adrenocorticotrophic Hormone (Acth) mentioning

confidence: 99%

The effects of sample handling andN‐phenylmaleimide on concentration of adrenocorticotrophic hormone in equine plasma

Rendle

Litchfield

Gough

et al. 2014

Equine Veterinary Journal

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“…Significant changes were observed for ACTH in the plasma fraction of human blood that is held at 4º or 24º C for up to 24 h before separation (Ellis et al, 2003). However, other human studies have shown that immediate centrifugation of plasma samples prolongs the stability of ACTH to 24 h even when stored at room temperature (Reisch et al, 2007). ACTH was found to be stable in whole blood from horses when held in plastic tubes for 8 h prior to separating the plasma (Perkins et al, 2002).…”

Section: Discussionmentioning

confidence: 92%

Influence of Collection and Storage Conditions on Adrenocorticotropic Hormone (ACTH) Measurements in Bottlenose Dolphins (Tursiops truncatus)

Lewis¹

2013

Aquat Mamm

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Identifying the effects of stressors on marine mammal health is critical for species conservation and collection. Adrenocorticotropic Hormone (ACTH) is a key indicator of peracute stress; however, handling methods may affect results. Thus, the aim of this study was to examine the effects of collection methods and storage temperature on the stability of bottlenose dolphin (Tursiops truncatus) ACTH measurements. Blood was collected from five managed-care bottlenose dolphins in four vacutainers ™ in the following sequence: EDTA, sodium heparin, serum separator, and 2nd EDTA. The specimens were centrifuged, processed immediately, aliquoted into plasma and serum, and divided into four equal volumes. These aliquots were immediately stored at either 4º or -80º C for 24 h prior to the ACTH assay. ACTH concentrations were compared between the collection tube types and the storage temperatures. There was a significant difference in ACTH concentration across collection tube types. ACTH concentrations were highest in ethylenediaminetetraacetic acid (EDTA) tubes and lowest in serum separator tubes at both temperatures. Twenty-four hour storage temperature did not have a statistically significant impact on mean ACTH concentration. There was no significant difference in ACTH concentration between the first and last drawn EDTA tubes. Bottlenose dolphin whole blood should be collected in EDTA for ACTH measurement. Samples may be refrigerated and shipped on cold packs if assayed within 24 h of collection or immediately frozen and shipped. When multiple samples are collected in various collection tubes at the same time, the order in which tubes are filled does not affect the ACTH measurement.

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Preanalytical Stability of Adrenocorticotropic Hormone Depends on Time to Centrifugation Rather than Temperature

Cited by 31 publications

References 5 publications

Current laboratory requirements for adrenocorticotropic hormone and renin/aldosterone sample handling are unnecessarily restrictive

Current laboratory requirements for adrenocorticotropic hormone and renin/aldosterone sample handling are unnecessarily restrictive

The effects of sample handling andN‐phenylmaleimide on concentration of adrenocorticotrophic hormone in equine plasma

Influence of Collection and Storage Conditions on Adrenocorticotropic Hormone (ACTH) Measurements in Bottlenose Dolphins (Tursiops truncatus)

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