Preanalytical temperature and storage stability of specific IgE antibodies in serum

Østergaard, Mette; Sandfeld-Paulsen, Birgitte

doi:10.1080/00365513.2023.2188606

Cited by 3 publications

(3 citation statements)

References 9 publications

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“…Moreover, studies have shown that the degradation of large peptides/proteins is minimized after several freeze–thaw cycles or long-term storage at − 80 °C, encouraging the use of valuable samples in archival biobanks for biomarker research 32 . Therefore, standardized sample preservation and management operations are crucial 17 , 33 , 34 .…”

Section: Discussionmentioning

confidence: 99%

“…Ultra-low temperature storage, an indispensable technology in BioBanks, facilitates the preservation of frozen serum or plasma, commonly utilized in retrospective studies 16 . However, concerns arise regarding the long-term storage of biological samples at such temperatures, as potential changes in concentration and composition may compromise the reliability of specific detections, such as sIgE, thereby posing challenges to ongoing research and diagnostic accuracy 17 – 19 . For instance, donkey serum has been demonstrated to remain stable when stored at − 80 °C for up to a year 16 .…”

Section: Introductionmentioning

confidence: 99%

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Impact of ultra-low temperature storage on serum sIgE detection and allergic disease biobank feasibility

Chang,

Hu,

Pan

et al. 2023

Sci Rep

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Research has shown that the concentration and composition of biological samples may change after long-term ultra-low temperature storage. Consequently, this study examined the effect of ultra-low temperature storage on serum sIgE detection by comparing sIgE concentrations at various durations from the time of sample storage to subsequent testing. We selected 40 serum samples from the Guangzhou Medical University Affiliated First Hospital Biobank, which had been tested for house dust mites, dog hair, tobacco mold, cockroaches, and cow milk allergen sIgE. Samples were categorized by storage duration: 14 samples stored for 10 years, 12 for 5 years, and 14 for 3 years. They were also classified by sIgE positive levels: 15 samples at levels 1–2, 15 at levels 3–4, and 10 at levels 5–6. The allergen sIgE of these samples was retested using the same technology. Regardless of the type of allergen or the level of positivity, the majority of sIgE concentrations measured at the time of storage were higher than the current measurements, but the difference was not statistically significant. The correlation between the sIgE results at the time of storage and the current results was high for samples stored for 10 years (rs = 0.991, P < 0.001) and 5 years (rs = 0.964, P < 0.001). Serum allergen sIgE is stable when stored under ultra-low temperature conditions, making the construction of a biological sample bank for allergic diseases feasible. This will facilitate researchers in quickly obtaining samples, conducting technical research, and translating findings, thereby promoting the development of the field of allergy through integration of industry, academia, and research.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Impact of ultra-low temperature storage on serum sIgE detection and allergic disease biobank feasibility

Chang,

Hu,

Pan

et al. 2023

Sci Rep

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show abstract

“…This is also a necessity for creating a serum sample bank for allergic patients. It has been proved that the allergen sIgE in serum can be stored stably at−20°C for 17 days ( 6 ), The study by Østergaard et al found that neither storage at 5°C for at least 10 days nor the type of storage tubes that were frozen for short periods of time affected sIgE levels ( 7 ). However, there is a lack of studies on the storage stability of serum allergen sIgE for longer periods of time under different temperature conditions.…”

Section: Introductionmentioning

confidence: 99%

Allergy patient-specific IgE antibody shows significantly stability during 3 months of storage at multiple temperatures from −80 to 25°C

Huang,

Zhu,

Xiao

et al. 2023

Front. Allergy

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The detection of allergen-specific IgE antibodies is an important biomarker for the diagnosis and treatment monitoring of allergic diseases. And the pre-analytical phase is an important part of the overall quality of the laboratory. In this study, 44 patients with allergic diseases (including 23 patients with allergic rhinitis, 12 patients with allergic rhinitis and asthma, and 9 patients with allergic dermatitis) were included in the outpatient center of the Department of Allergy, the First Affiliated Hospital of Guangzhou Medical University. We mixed the serums of the above 44 patients (approximately 0.8 ml of serum volume per patient) into a large volume of serum pool (about 35 ml in total) and divided into 26 parts. And 26 serum samples were stored at 4 different temperatures for 90 days to observe the stability of sIgE antibodies to 16 allergens in serum. The results show that serum sIgE antibody titers in patients with allergic diseases show significant stability during 90 days of storage, even at room temperature. Good stability even after up to 10 freeze-thaw cycles under low temperature storage conditions.

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Pre-analytical stability of ocrelizumab in serum after delayed centrifugation of whole blood

Shim,

Carrasco-Triguero,

Fischer

2024

AAPS Open

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Preanalytical temperature and storage stability of specific IgE antibodies in serum

Cited by 3 publications

References 9 publications

Impact of ultra-low temperature storage on serum sIgE detection and allergic disease biobank feasibility

Impact of ultra-low temperature storage on serum sIgE detection and allergic disease biobank feasibility

Allergy patient-specific IgE antibody shows significantly stability during 3 months of storage at multiple temperatures from −80 to 25°C

Pre-analytical stability of ocrelizumab in serum after delayed centrifugation of whole blood

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