Prebiotic agavin in juvenile totoaba,
            <i>Totoaba macdonaldi</i>
            diets, to relieve soybean meal‐induced enteritis: Growth performance, gut histology and microbiota

Fuentes‐Quesada, José Pablo; Cornejo‐Granados, Fernanda; Mata-Sotres, José Antonio; Ochoa‐Romo, Juan Pablo; Rombenso, Artur N.; Guerrero‐Rentería, Yanet; Lazo, Juan Pablo; Pohlenz, Camilo; Ochoa‐Leyva, Adrián; Viana, Marı́a Teresa

doi:10.1111/anu.13151

Cited by 19 publications

(8 citation statements)

References 86 publications

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“…The reduction in microbial population diversity is usually observed in diseased fish when compared to healthy individuals [ 49 ], and this decrease also suggests the effectiveness of the protective barrier provided by the commensal microbiota could have been compromised [ 50 ]. However, similar to the present study, a lower diversity was observed for totoaba treated with diets containing moderate inclusion levels of soybean meal and supplemented with the prebiotic Agavin [ 51 ]. The high relative abundance of Pediococcus spp., as observed in the relative abundance taxa plot, may be the main driver of the reduced diversity for the intestinal microbiota of prebiotic and Pre + Pro fed red drum.…”

Section: Discussionsupporting

confidence: 88%

Dietary Supplementation of a Commercial Prebiotic, Probiotic and Their Combination Affected Growth Performance and Transient Intestinal Microbiota of Red Drum (Sciaenops ocellatus L.)

Yamamoto

Ellis

Bowles

et al. 2022

Animals

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In the present study, the potential synergism between beneficial lactic acid bacteria (Pediococcus acidilactici) contained in a probiotic and a mixture of fermentable complex carbohydrates and autolyzed brewer’s yeast (or prebiotic) were explored in red drum. Four experimental diets were formulated from practical ingredients, and the basal diet was supplemented with either probiotic, prebiotic, or both supplements. Red drum juveniles (~5.5 g) were offered the four experimental diets for 56 days, and at the end of the feeding trial fish fed diets supplemented with probiotic had significantly better weight gain than those fed the non-supplemented diets, and higher protein content in their whole-body composition. Transient intestinal microbiome alpha and beta diversity were significantly affected by the dietary treatments. Interestingly, a higher relative abundance of the lactic acid genus Pediococcus was observed for fish fed diets supplemented with the prebiotic. A higher relative abundance was also observed for the predicted functions of the microbial metagenome, and many of these pathways involved the biosynthesis of essential amino acids, vitamins, and nucleotides. Even though no potential synergistic effect was observed, the individual inclusion of these prebiotic and probiotic supplements positively affected the intestinal health and growth performance of red drum, respectively.

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Section: Discussionsupporting

confidence: 88%

Dietary Supplementation of a Commercial Prebiotic, Probiotic and Their Combination Affected Growth Performance and Transient Intestinal Microbiota of Red Drum (Sciaenops ocellatus L.)

Yamamoto

Ellis

Bowles

et al. 2022

Animals

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“…This improvement in the FCA could be related to the significantly higher protein efficiency ratio in the diet obtained with a 2% agavin enriched diet compared to the basal diet, suggesting that changes in the microbiota could be associated with increased protein intake efficiency. In this regard, the same effect was previously observed in the fish Totoaba macdonaldi using diets enriched with agavin 51 . Interestingly, a higher proportion of agavin in the shrimp diet (AG10) maintains the same growth performance parameters as the shrimps fed with the BD diet.…”

Section: Discussionsupporting

confidence: 83%

“…Interestingly, the protein efficiency ratio was significantly higher in the diet with 2% agavin than the control, suggesting that the enriched Bradyrhizobium could participate in nitrogen fixation, triggering the increase in protein efficiency ratio. A similar process was recently proposed for Totoaba macdonaldi , a predatory fish in which a diet supplemented with 2% agavin also showed higher protein efficiency via enrichment of Sinorhizobium , a nitrogen-fixing bacteria genus 51 . On the other hand, the Bacillaceae family and specifically the Bacillus genus were enriched in the intestines of the AG10 diet.…”

Section: Discussionmentioning

confidence: 53%

Agavin induces beneficial microbes in the shrimp microbiota under farming conditions

Ochoa‐Romo

Cornejo‐Granados

López-Zavala

et al. 2022

Sci Rep

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Prebiotics and probiotics have shown a number of beneficial impacts preventing diseases in cultured shrimps. Complex soluble carbohydrates are considered ideal for fostering microbiota biodiversity by fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPS). Here we evaluated the growth performance and microbiota composition of the white shrimp Litopenaeus vannamei after dietary intervention using agavin as a FODMAP prebiotic under farming conditions. Adult L. vannamei were raised at a shrimp farm and the effect of agavin supplemented at 2% (AG2) or 10% (AG10) levels were compared to an agavin-free basal diet (BD). After 28 days-trial, the feed conversion ratio, total feed ingested, and protein efficiency ratio was significantly improved on animals fed with AG2. At the same time, no effect on growth performance was observed in AG10. Surprisingly, after sequencing the V3–V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD. The beta diversity analysis showed a clear significant microbiota clustering by agavin diets only in the hepatopancreas, suggesting that agavin supplementation had a more substantial deterministic effect on the microbiota of hepatopancreas than on the intestine. We analyzed the literature to search beneficial microbes for shrimp’s health and found sequences for 42 species in our 16S data, being significantly increased Lactobacillus pentosus, Pseudomonas putida and Pseudomonas synxantha in the hepatopancreas of the AG10 and Rodopseudomonas palustris and Streptococcus thermophiles th1435 in the hepatopancreas of the AG2, both compared to BD. Interestingly, when we analyzed the abundance of 42 beneficial microbes as a single microbial community "meta-community," found an increase in their abundance as agavin concentration increases in the hepatopancreas. In addition, we also sequenced the DNA of agavin and found 9 of the 42 beneficial microbes. From those, Lactobacillus lactis and Lactobacillus delbrueckii were found in shrimps fed with agavin (both AG2 and AG10), and Lysinibacillus fusiformis in AG10 and they were absent the BD diet, suggesting these three species could be introduced with the agavin to the diet. Our work provides evidence that agavin supplementation is associated with an increase of beneficial microbes for the shrimp microbiota at farming conditions. Our study provides the first evidence that a shrimp prebiotic may selectively modify the microbiota in an organ-dependent effect.

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“…Barreto‐Curiel et al (2018) characterized the intestinal microbiota of totoabas (±7.7 g) fed with extruded aquafeed where the Proteobacteria/Firmicutes ratio was 70%:25%. Fuentes‐Quesada et al (2020) characterized the intestinal microbiota of T . macdonaldi (60.8 ± 2.3 g) fed with soybean meal for 44 days and found Phylum Proteobacteria (±60%) and Firmicutes (±30%) as dominant groups of the transient microbiota.…”

Section: Discussionmentioning

confidence: 99%

Soy protein concentrate effects on gut microbiota structure and digestive physiology of Totoaba macdonaldi

Larios‐Soriano¹,

Zavala²,

López³

et al. 2021

J of Applied Microbiology

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Aims: Examine the effect of soy protein concentrate (SPC) on allochthonous microbiota, hindgut integrity, and liver tissue of totoaba (Totoaba macdonaldi).Methods and Results: Four diets were prepared: control diet (100% fishmeal) and experimental diets containing partial substitution of fishmeal by SPC (15%, 30% and 45% SPC). After 90 days, samples of the hindgut contents were taken to determine the taxonomic composition of the allochthonous microbiota through sequencing of the V3-V4 region of the 16S rRNA gene. Simultaneously, liver and hindgut samples were collected for examination by histological approaches. The SPC modulated the richness and abundance of the accessory microbiota, of which the main operational taxonomic unit showed an increase corresponding to the Phylum Firmicutes (Bacillales and Lactobacillales). With the increase in SPC, a slight decrease in mucosal fold width, a decrease in goblet cells and a slight distortion of the villi in the hindgut were observed. In the liver, SPC was observed to influence hepatocytes morphology through irregular and enlarged nuclei. Conclusion:The study demonstrates that Proteobacteria dominated the allochthonous microbiota of subadult totoaba, regardless of the diet. However, the SPC modulated the accessory bacteria communities and caused slight effects on the liver and gut of fish. Significances and Impact of the Study:To our knowledge, this is the first study that analyses the effects of SPC on allochthonous microbiota of subadults T. macdonaldi through new generation techniques such as DNA sequencing for metagenomic analysis.

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Prebiotic agavin in juvenile totoaba, Totoaba macdonaldi diets, to relieve soybean meal‐induced enteritis: Growth performance, gut histology and microbiota

Abstract: Most commercial diets tend to reduce fishmeal (FM) levels, where the poultry by-product meal (PBM) has been proved to be useful as partial replacement of FM

Cited by 19 publications

References 86 publications

Dietary Supplementation of a Commercial Prebiotic, Probiotic and Their Combination Affected Growth Performance and Transient Intestinal Microbiota of Red Drum (Sciaenops ocellatus L.)

Dietary Supplementation of a Commercial Prebiotic, Probiotic and Their Combination Affected Growth Performance and Transient Intestinal Microbiota of Red Drum (Sciaenops ocellatus L.)

Agavin induces beneficial microbes in the shrimp microbiota under farming conditions

Soy protein concentrate effects on gut microbiota structure and digestive physiology of Totoaba macdonaldi

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