Precipitating Antibody in Human Serum to an Antigen Present in Cultured Burkitt's Lymphoma Cells

Old, Lloyd J.; Boyse, Edward A.; Oettgen, Herbert F.; Harven, Étienne de; Geering, Gayla; Williamson, Barbara; Clifford, Peter

doi:10.1073/pnas.56.6.1699

Cited by 448 publications

(153 citation statements)

References 17 publications

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“…There is now both serological and cellular evidence (Anderson-Anvret et al, 1978;Klein, 1979;Saemundsen et al, 1982) in support of the association reported by Old et al (1966) (Klein, 1979).…”

supporting

confidence: 50%

Space-time clustering of nasopharyngeal carcinoma in Greenland Eskimos

Albeck¹,

Coleman²,

Nielsen³

et al. 1985

Br J Cancer

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supporting

confidence: 50%

Space-time clustering of nasopharyngeal carcinoma in Greenland Eskimos

Albeck¹,

Coleman²,

Nielsen³

et al. 1985

Br J Cancer

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“…Despite this predilection for the B cell system, the strongest and best documented link between EBV and a human cancer involves an epithelial tumour, undifferentiated nasopharyngeal carcinoma (NPC). The association with NPC, first revealed by serological evidence (Old et al, 1966), was later reinforced by nucleic acid hybridization data indicating that EBV DNA was consistently detectable in NPC biopsy material (zur Hausen et al, 1970;Nonoyama et al, 1973). Separation of the malignant epithelial cells from infiltrating lymphocytes either by cell culture (Wolf et al, 1973;Huang et al, 1974) or 0000-8170 © 1988 SGM by passage in nude mice (Klein et al, 1974) subsequently showed that the EBV genome, and indeed an EBV-coded nuclear antigen (EBNA), could be detected in the tumour cells.…”

Section: Introductionmentioning

confidence: 97%

Epstein--Barr Virus Gene Expression in Nasopharyngeal Carcinoma

Lw¹,

Dawson²,

Clark³

et al. 1988

Journal of General Virology

434

236

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SUMMARYEpstein-Barr virus (EBV), an agent with growth transforming potential for human B cells, is associated with certain B cell lymphomas in man and also with an epithelial tumour, undifferentiated nasopharyngeal carcinoma (NPC). Since B cell growth transformation is associated with the constitutive expression of a small number of EBV-coded latent proteins, the nuclear antigens EBNA 1, EBNA 2, EBNA 3 and EBNA-LP and the latent membrane protein (LMP), the present work sought to determine whether this same pattern of virus gene expression occurred in NPC. Tumour biopsies were taken from NPC patients from three areas of differing tumour incidence (Kenya, Algeria, Britain) and immediately snap-frozen, as were biopsies of non-EBV-related carcinomas for controls. Immunoblotting of PAGE-separated proteins with selected human sera identified 24 NPC biopsies clearly expressing EBNA 1. When the analysis was extended using selected human sera with antibodies against the other EBNAs, there was no detectable expression of EBNA 2, EBNA 3 or EBNA-LP in any of these 24 biopsies; their EBNA 2-negative status was confirmed using a monoclonal antibody (MAb) PE2 which was reactive in immunoblotting and in immunoprecipitation with EBNA 2A and EBNA 2B proteins. Similar experiments with two different LMP-specific MAbs, CS1 to 4 and S12, revealed heterogeneity between NPC biopsies; 9/24 biopsies were demonstrably LMP-positive, the degree of expression varying considerably between individual tumours in a manner which was not related to the level of EBNA 1 expression. None of the 24 NPC biopsies expressed detectable amounts of EBV lytic cycle antigens. A nude mouse-passaged NPC cell line, C15, likewise expressed EBNA 1 and LMP but none of the other EBV latent proteins nor lytic cycle antigens. This work identifies a novel type of EBV-cell interaction in NPC cells which is distinct from that seen in in vitro transformed B cell lines and from that seen to date in EBV-positive B cell lymphomas.

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“…Old et al (1966) first demonstrated the presence of precipitating antibodies to EBV-related antigens in sera from patients with the cancer. This discovery was followed by the demonstration that NPC patients in widely separated parts of the world had higher geometric mean titres (GMT) of antibodies to EB viral capsid antigen (VCA) than those of control groups, made up of patients with other head and neck cancers and normal subjects (de Schryver et al, 1969(de Schryver et al, .…”

mentioning

confidence: 99%

Epstein-Barr-virus-specific IgA and IgG serum antibodies in nasopharyngeal carcinoma

Ho¹,

Ng²,

Kwan³

et al. 1976

Br J Cancer

134

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Summary.-The sera of 73 patients with nasopharyngeal carcinoma (NPC), 28 patients with other carcinomas (OC) and 89 healthy subjects (HS) were tested for IgG and IgA antibodies to Epstein-Barr virus (EBV) viral capsid antigen (VCA). The majority of the NPC sera had IgG titres of 160 or above, whereas the majority of the other sera had titres below 160. For IgA reactivity to EBV-VCA,68 of 73 (93*2%) NPC sera had titres of > 10. In contrast, only 6 of 28 (21-4%) OC sera and none of the HS sera had such titres. The mean serum concentrations of IgG, IgA, IgM and C3' were also determined in 55 NPC and 20 OC patients and 18 HS. They were all significantly higher in the NPC sera than in the HS. Although the concentrations of IgG and C3' were not significantly different in the two carcinoma groups, the concentrations of IgA and IgM were significantly higher in the NPC group than in OC. These findings appear to reflect the intensity of EBV-specific antigenic stimulation in NPC, and the EBV-specific serum IgA reactivity may be a useful aid to the diagnosis of NPC, especially in cases with an occult primary tumour. It may be also of value as a screening test in people at high risk.

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Precipitating Antibody in Human Serum to an Antigen Present in Cultured Burkitt's Lymphoma Cells

Cited by 448 publications

References 17 publications

Space-time clustering of nasopharyngeal carcinoma in Greenland Eskimos

Space-time clustering of nasopharyngeal carcinoma in Greenland Eskimos

Epstein--Barr Virus Gene Expression in Nasopharyngeal Carcinoma

Epstein-Barr-virus-specific IgA and IgG serum antibodies in nasopharyngeal carcinoma

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