Precise base editing of non-allelic acetolactate synthase genes confers sulfonylurea herbicide resistance in maize

Li, Yanmin; Zhu, Jinjie; Wu, Hao; Liu, Changlin; Huang, Changling; Lan, Jinhao; Zhao, Yanming; Xie, Chuanxiao

doi:10.1016/j.cj.2019.10.001

Cited by 69 publications

(36 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the products of transgenic approach are GM, and the food safety, environmental, and biosafety management procedures for GM products are cumbersome [ 93 ]. Although the safety regulation of genome editing products is still controversial [ 32 ], the edited plants without the introduction of exogenous genes [ 94 ] have not been regulated in many countries, offering an advantage over plants generated by transgenic approaches [ 95 ].…”

Section: Mechanism and Approaches For Development Of Herbicide Resistant Plants/cropsmentioning

confidence: 99%

“…Another research using the same base editing system in maize achieved the targeted amino acid substitution in two nonallelic ZmALS1 and ZmALS2 genes. Intriguingly, the targeted mutations in these genes conferred significantly higher resistance to chlorsulfuron herbicide in maize [ 95 ]. Another base editing tool, adenine base editing (ABE), has been used in rice by Liu and team.…”

Section: Mechanism and Approaches For Development Of Herbicide Resistant Plants/cropsmentioning

confidence: 99%

See 1 more Smart Citation

Herbicide Resistance: Another Hot Agronomic Trait for Plant Genome Editing

Hussain

Ding

Alariqi

et al. 2021

Plants

View full text Add to dashboard Cite

Weeds have continually interrupted crop plants since their domestication, leading to a greater yield loss compared to diseases and pests that necessitated the practice of weed control measures. The control of weeds is crucial to ensuring the availability of sufficient food for a rapidly increasing human population. Chemical weed control (herbicides) along with integrated weed management (IWM) practices can be the most effective and reliable method of weed management programs. The application of herbicides for weed control practices calls for the urgency to develop herbicide-resistant (HR) crops. Recently, genome editing tools, especially CRISPR-Cas9, have brought innovation in genome editing technology that opens up new possibilities to provide sustainable farming in modern agricultural industry. To date, several non-genetically modified (GM) HR crops have been developed through genome editing that can present a leading role to combat weed problems along with increasing crop productivity to meet increasing food demand around the world. Here, we present the chemical method of weed control, approaches for herbicide resistance development, and possible advantages and limitations of genome editing in herbicide resistance. We also discuss how genome editing would be effective in combating intensive weed problems and what would be the impact of genome-edited HR crops in agriculture.

show abstract

Section: Mechanism and Approaches For Development Of Herbicide Resistant Plants/cropsmentioning

confidence: 99%

Section: Mechanism and Approaches For Development Of Herbicide Resistant Plants/cropsmentioning

confidence: 99%

Herbicide Resistance: Another Hot Agronomic Trait for Plant Genome Editing

Hussain

Ding

Alariqi

et al. 2021

Plants

View full text Add to dashboard Cite

show abstract

“…Both CBE and ABE have been successfully applied in various model plants and crops ( Chen et al., 2019 ). They are widely used to introduce targeted substitutions in major genes to improve important agricultural traits, including plant height, flowering time, disease resistance, and herbicide resistance ( Chen et al., 2017 , Lu and Zhu, 2017 , Shimatani et al., 2017 , Kang et al., 2018 , Li et al., 2018 , Li et al., 2019a , Tian et al., 2018 , Bastet et al., 2019 , Zhang et al., 2019 , Wu et al., 2020 ). Base editors were also employed to disrupt genes in plants by creating early stop codons or inducing transcript mis-splicing ( Kang et al., 2018 , Xue et al., 2018 , Li et al., 2019b , Veillet et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Development of Plant Prime-Editing Systems for Precise Genome Editing

Liu

et al. 2020

Plant Communications

165

123

View full text Add to dashboard Cite

Prime-editing systems have the capability to perform efficient and precise genome editing in human cells. In this study, we first developed a plant prime editor 2 (pPE2) system and test its activity by generating a targeted mutation on an HPT -ATG reporter in rice. Our results showed that the pPE2 system could induce programmable editing at different genome sites. In transgenic T 0 plants, pPE2-generated mutants occurred with 0%–31.3% frequency, suggesting that the efficiency of pPE2 varied greatly at different genomic sites and with prime-editing guide RNAs of diverse structures. To optimize editing efficiency, guide RNAs were introduced into the pPE2 system following the PE3 and PE3b strategy in human cells. However, at the genomic sites tested in this study, pPE3 systems generated only comparable or even lower editing frequencies. Furthemore, we developed a surrogate pPE2 system by incorporating the HPT -ATG reporter to enrich the prime-edited cells. The nucleotide editing was easily detected in the resistant calli transformed with the surrogate pPE2 system, presumably due to the enhanced screening efficiency of edited cells. Taken together, our results indicate that plant prime-editing systems we developed could provide versatile and flexible editing in rice genome.

show abstract

“…Herbicide tolerance has been incorporated into the allotetraploid oilseed rape targeting the ALS gene with a human A3A cytidine deaminase or a rat cytidine deaminase fused to a Cas9 nickase and uracil glycosylase inhibitor (Cheng et al, 2020; Wu et al, 2020). CBE systems have also been used to mutagenize the ALS gene to produce herbicide‐tolerant maize and watermelon (Tian et al, 2018; Li et al, 2020g). Tolerance to sulfonylurea‐, imidazolinone‐ and aryloxyphenoxy propionate‐type herbicides has been achieved in wheat by targeting the ALS and acetyl‐coenzyme A carboxylase genes with a CBE system (Zhang et al, 2019c).…”

Section: Base Editingmentioning

confidence: 99%

Genome editing for plant research and crop improvement

Zhan¹,

Zhu

et al. 2021

JIPB

View full text Add to dashboard Cite

The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a profound impact on plant biology, and crop improvement. In this review, we summarize the state-of-the-art development of CRISPR technologies and their applications in plants, from the initial introduction of random small indel (insertion or deletion) mutations at target genomic loci to precision editing such as base editing, prime editing and gene targeting. We describe advances in the use of class 2, types II, V, and VI systems for gene disruption as well as for precise sequence alterations, gene transcription, and epigenome control.

show abstract

Precise base editing of non-allelic acetolactate synthase genes confers sulfonylurea herbicide resistance in maize

Cited by 69 publications

References 31 publications

Herbicide Resistance: Another Hot Agronomic Trait for Plant Genome Editing

Herbicide Resistance: Another Hot Agronomic Trait for Plant Genome Editing

Development of Plant Prime-Editing Systems for Precise Genome Editing

Genome editing for plant research and crop improvement

Contact Info

Product

Resources

About