Precise coexistence of regulatory peptides in the nerve fibers of the amphibian carotid labyrinth demonstrated by a combination of double immunofluorescence labelling and a multiple dye filter

Kusakabe, Tatsumi; Kawakami, Tadashi; Ono, Michio; Syoui, Noriko; Kurihara, Katsuyoshi; Takenaka, Toshifumi; Sawada, Hajime

doi:10.1016/0006-8993(96)00788-3

Cited by 4 publications

(2 citation statements)

References 15 publications

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“…The specimens were then cut at 10 m on a cryostat and mounted on poly-L-lysine-coated slides. The sections were processed for indirect double-labeling immunofluorescence as previously described (24). In brief, they were first incubated overnight at 4°C with guinea pig anti-NOS polyclonal antibody (1:1,000) and then incubated under the same conditions with rabbit antiserum directed against CGRP (1:1,500).…”

Section: Methodsmentioning

confidence: 99%

CGRP modulates mucin synthesis in surface mucus cells of rat gastric oxyntic mucosa

Ichikawa¹,

Ishihara

Kusakabe

et al. 2000

American Journal of Physiology-Gastrointestinal and Liver Physi

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We examined the effects of the calcitonin gene-related peptide (CGRP), including the possible participation of nitric oxide (NO), on mucin biosynthesis in the surface epithelium and remaining deep mucosa as well as the entire mucosa and compared the distribution of CGRP and NO synthase (NOS) using a combination of double immunofluorescence labeling and multiple dye filter. Pieces of tissue obtained from the corpus and antrum were incubated in a medium containing [(3)H]glucosamine and CGRP, with or without the NOS inhibitor. CGRP dose-dependently enhanced [(3)H]glucosamine incorporation into the corpus mucin but had no effect on antral mucin biosynthesis. The CGRP receptor antagonist, CGRP-(8-37), prevented the increase in (3)H-labeled corpus mucin. This stimulation of corpus mucin synthesis disappeared after removal of the surface mucus cell layer. CGRP activated the mucin biosynthesis in the surface mucus cells. In the full-thickness corpus mucosa, CGRP-induced activation was completely blocked by the NOS inhibitor. CGRP-immunoreactive fibers were intertwined within the surface mucus cell layer with type I NOS immunoreactivity. These results show that CGRP-stimulated mucin biosynthesis mediated by NO is limited to surface mucus cells of the rat gastric oxyntic mucosa.

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Section: Methodsmentioning

confidence: 99%

CGRP modulates mucin synthesis in surface mucus cells of rat gastric oxyntic mucosa

Ichikawa¹,

Ishihara

Kusakabe

et al. 2000

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…Recently, Kusakabe et al (1991Kusakabe et al ( , 1993aKusakabe et al ( , 1995b found several immunoreactive neuropeptides in the nerve fibers within the carotid labyrinths of several amphibian species and suggested that peptidergic innervation may also participate in the vascular regulatory function. The ontogeny and precise coexistence of several neuropeptides in the labyrinth have also been observed (Kusakabe, 1992c;Kusakabe et al, 1993cKusakabe et al, , 1994aKusakabe et al, , 1996d.…”

Section: Introductionmentioning

confidence: 92%

Carotid labyrinth of amphibians

Kusakabe

2002

Microscopy Res & Technique

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The amphibian carotid labyrinth is a characteristic maze-like vascular expansion at the bifurcation of the common carotid artery into the internal and external carotid arteries. The carotid labyrinths of anurans are spherical and those of urodeles are oblong. In the intervascular stroma of both anuran and urodelan carotid labyrinths, the glomus cells (type I cells, chief cells) are distributed singly or in clusters between connective tissue cells and smooth muscle cells. In fluorescence histochemistry, the glomus cells emit intense fluorescence for biogenic monoamines. In fine structure, the glomus cells are characterized by a number of dense-cored vesicles in their cytoplasm. The glomus cells have long, thin cytoplasmic processes, some of which are closely associated with smooth muscle cells, endothelial cells, and pericytes. Afferent, efferent, and reciprocal synapses are found on the glomus cells. The morphogenesis of the carotid labyrinth starts in the larvae at the point where the carotid arch descends to the internal gills. Through the early stages of larval development, the slightly expanded region of the external carotid artery becomes closely connected with the carotid arch. By the end of the foot stage, the expanded region becomes globular, and at the final stage of metamorphosis the carotid labyrinth is close to its adult form. In fine structure, the glomus cells appear as early as the initial stage of larval development. At the middle stages of development, the number of dense-cored vesicles increases remarkably. Distinct afferent synapses are found in juveniles, although efferent synapses can be seen during metamorphosis. The carotid labyrinth is innervated by nerve fibers containing several kinds of regulatory neuropeptides. Double-immunolabeling in combination with a multiple dye filter system demonstrates the coexistence of two different neuropeptides. The amphibian carotid labyrinth has been electrophysiologically confirmed to have arterial chemo- and baroreceptor functions analogous to those of the mammalian carotid body and carotid sinus. The ultrastructural characteristics of the glomus cells during and after metamorphosis suggest that the glomus cells contribute to the chemoreception after metamorphosis. The three-dimensional fine structure of vascular corrosion casts suggests that the amphibian carotid labyrinth has the appropriate architecture for controlling vascular tone and the findings throughout metamorphosis reveal that the vascular regulatory function begins at an early stage of metamorphosis. In addition, immunohistochemical studies suggest that the vascular regulation in the carotid labyrinth is under peptidergic innervation. Thus, the multiple functions of the carotid labyrinth underline the importance of this relatively small organ for maintenance of homeostasis and appropriate blood supply to the cephalic region.

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The Amphibian Carotid Labyrinth

Kusakabe¹

2019

Airway Chemoreceptors in the Vertebrates

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Precise coexistence of regulatory peptides in the nerve fibers of the amphibian carotid labyrinth demonstrated by a combination of double immunofluorescence labelling and a multiple dye filter

Cited by 4 publications

References 15 publications

CGRP modulates mucin synthesis in surface mucus cells of rat gastric oxyntic mucosa

CGRP modulates mucin synthesis in surface mucus cells of rat gastric oxyntic mucosa

Carotid labyrinth of amphibians

The Amphibian Carotid Labyrinth

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