2019
DOI: 10.1038/s41587-019-0013-6
|View full text |Cite
|
Sign up to set email alerts
|

Precise RNA editing by recruiting endogenous ADARs with antisense oligonucleotides

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

2
179
0
1

Year Published

2019
2019
2022
2022

Publication Types

Select...
3
3
3

Relationship

1
8

Authors

Journals

citations
Cited by 215 publications
(190 citation statements)
references
References 40 publications
2
179
0
1
Order By: Relevance
“…Several systems were recently developed to recruit ADAR enzymes to specific sites for site-directed RNA editing [23][24][25][26][27][28][29] , providing novel tools to study biological function and a safer and reversible alternative to gene therapy 23,[47][48][49] . These RNA engineering approaches use antisense RNA oligoribonucleotides as guide RNAs (gRNAs) to recruit either engineered or endogenous ADAR enzymes.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Several systems were recently developed to recruit ADAR enzymes to specific sites for site-directed RNA editing [23][24][25][26][27][28][29] , providing novel tools to study biological function and a safer and reversible alternative to gene therapy 23,[47][48][49] . These RNA engineering approaches use antisense RNA oligoribonucleotides as guide RNAs (gRNAs) to recruit either engineered or endogenous ADAR enzymes.…”
Section: Discussionmentioning
confidence: 99%
“…Predictive models derived through systematic mutagenesis would not only promote our understanding of the most prevalent type of RNA editing, but would also greatly advance the emerging field of ADAR-mediated transcriptome engineering, by informing the design of antisense RNA guides for therapeutic RNA editing. Prototypes of ADAR RNA guides were recently demonstrated to enable programmable RNA editing by forming dsRNA with target sites and recruiting endogenous ADAR proteins [23][24][25][26][27][28][29] . Improving the specificity and efficiency of programmable editing through rational design of guide/target duplexes will be critical as this new technology continues to be developed for clinical applications.…”
Section: Introductionmentioning
confidence: 99%
“…Additionally, development of treatments specifically aimed at the reversal of deleterious editing effects can also be pursued, e.g., via a combination of short‐term stimulation of expression of ADAR editing targets, administration of antagonists to the edited protein versions, and/or temporary suppression of ADAR editing. Replacement‐focused therapies similar to those of paralysis treatment via stimulation of neural growth or directed editing could prove promising too …”
Section: Other Future Research Prospectsmentioning
confidence: 99%
“…Many of these studies utilized fusions of the ADAR deaminase domain with a heterologous RNA binding protein, for example Cas13 or the bacteriophage λN peptide, with a RNA guide specific for the target RNA substrate [13,[15][16][17][18][19][20][21]. Endogenous RNAs have been shown to be repaired by programmable RNA editing in cell lines and primary cells [17][18][19]22]. A recent study demonstrated repair in mouse models of Duchenne muscular dystrophy and ornithine transcarbamylase deficiency [21], establishing the utility of this approach for both muscle and liver.…”
Section: Introductionmentioning
confidence: 99%