Precise role of dermal fibroblasts on melanocyte pigmentation

Wang, Yinjuan; Viennet, Céline; Robin, Sophie; Berthon, Jean‐Yves; He, Li; Humbert, Philippe

doi:10.1016/j.jdermsci.2017.06.018

Cited by 124 publications

(112 citation statements)

References 89 publications

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“…Our results are consistent with previous work by Kim et al (), who suggested that temperature may affect the activity of key melanogenic enzyme tyrosinase. However, further research is required to gain more insights on the possible association between fibroblast‐melanocyte crosstalk in HSEs (Wang et al, ), lower epidermal region disorganization, culture temperature, and pigmentation.…”

Section: Discussionmentioning

confidence: 99%

Characterization of human skin equivalents developed at body's core and surface temperatures

Mieremet

Dijk

Boiten

et al. 2019

J Tissue Eng Regen Med

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Human skin equivalents (HSEs) are in vitro developed three‐dimensional models resembling native human skin (NHS) to a high extent. However, the epidermal lipid biosynthesis, barrier lipid composition, and organization are altered, leading to an elevated diffusion rate of therapeutic molecules. The altered lipid barrier formation in HSEs may be induced by standardized culture conditions, including a culture temperature of 37°C, which is dissimilar to skin surface temperature. Therefore, we aim to determine the influence of culture temperature during the generation of full thickness models (FTMs) on epidermal morphogenesis and lipid barrier formation. For this purpose, FTMs were developed at conventional core temperature (37°C) or lower temperatures (35°C and 33°C) and evaluated over a time period of 4 weeks. The stratum corneum (SC) lipid composition was analysed using advanced liquid chromatography coupled to mass spectrometry analysis. Our results show that SC layers accumulated at a similar rate irrespective of culture temperature. At reduced culture temperature, an increased epidermal thickness, a disorganization of the lower epidermal cell layers, a delayed early differentiation, and an enlargement of granular cells were detected. Interestingly, melanogenesis was reduced at lower temperature. The ceramide subclass profile, chain length distribution, and level of unsaturated ceramides were similar in FTMs generated at 37°C and 35°C but changed when generated at 33°C, reducing the resemblance to NHS. Herein, we report that culture temperature affects epidermal morphogenesis substantially and to a lesser extent the lipid barrier formation, highlighting the importance of optimized external parameters during reconstruction of skin.

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Section: Discussionmentioning

confidence: 99%

Characterization of human skin equivalents developed at body's core and surface temperatures

Mieremet

Dijk

Boiten

et al. 2019

J Tissue Eng Regen Med

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“…Keratinocytes will distribute melanin to the upper parts of the skin layer . Wang et al (2017) also mentioned that hormonal factors, family history and cosmetics are the three main triggering and aggravating factors for melanoma development. When a primary melanoma is formed, it activates some signals, which will provide conditions that make the primary tumor strong such as: (i) attachment to the wall of the blood and/or lymphatic vessels, enabling the movement through a new organ; (ii) enough nutrients to grow; and (iii) it is resistant to the immune system.…”

Section: Melanomamentioning

confidence: 99%

Melanoma in the Eyes of Mechanobiology

Brás

Radmacher

Sousa

et al. 2020

Front. Cell Dev. Biol.

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Skin is the largest organ of the human body with several important functions that can be impaired by injury, genetic or chronic diseases. Among all skin diseases, melanoma is one of the most severe, which can lead to death, due to metastization. Mechanotransduction has a crucial role for motility, invasion, adhesion and metastization processes, since it deals with the response of cells to physical forces. Signaling pathways are important to understand how physical cues produced or mediated by the Extracellular Matrix (ECM), affect healthy and tumor cells. During these processes, several molecules in the nucleus and cytoplasm are activated. Melanocytes, keratinocytes, fibroblasts and the ECM, play a crucial role in melanoma formation. This manuscript will address the synergy among melanocytes, keratinocytes, fibroblasts cells and the ECM considering their mechanical contribution and relevance in this disease. Mechanical properties of melanoma cells can also be influenced by pigmentation, which can be associated with changes in stiffness. Mechanical changes can be related with the adhesion, migration, or invasiveness potential of melanoma cells promoting a high metastization capacity of this cancer. Mechanosensing, mechanotransduction, and mechanoresponse will be highlighted with respect to the motility, invasion, adhesion and metastization in melanoma cancer.

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“…Moreover, agents known to improve the levels of collagen will likely counteract the pilosebaceous pore wall slackening that occurs in conspicuous pores. In addition, paracrine signalling molecules induced by UV irradiation on keratinocytes and fibroblasts induce melanogenesis . Also, the effects of UV on increasing sebaceous gland activity should not be underestimated in the expression of skin pores also .…”

Section: Discussionmentioning

confidence: 99%

“…In an attempt to provide greater insight into the potential mechanism of action of HSA, particularly on the potential paracrine effect of fibroblasts after treatment, on the expression of pore and age spot problems, we examined changes in secretome of fibroblasts (cell culture conditioned media), determined by mass spectrometry‐based proteomics, that might influence the fibroblasts in an autocrine fashion or keratinocytes or melanocytes in a paracrine fashion . Dermal fibroblasts play a key role in ECM formation and melanocyte pigmentation .…”

Section: Discussionmentioning

confidence: 99%

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Bio‐derived hydroxystearic acid ameliorates skin age spots and conspicuous pores

Schütz

Rawlings²,

Wandeler

et al. 2019

Intern J of Cosmetic Sci

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IntroductionWe report on the preparation and efficacy of 10‐hydroxystearic acid (HSA) that improves facial age spots and conspicuous pores.MethodsThe hydration of oleic acid into HSA was catalyzed by the oleate hydratase from Escherichia coli. Following treatment with HSA, collagen type I and type III was assessed in primary human dermal fibroblasts together with collagen type III, p53 protein levels and sunburn cells (SBC) after UVB irradiation (1 J cm−2) by immunohistochemistry on human ex vivo skin. UVB‐induced expression of matrix metalloprotease‐1 (MMP‐1) was determined from full thickness skin by RT‐qPCR. Modification of the fibroblast secretome by HSA was studied by mass‐spectrometry‐based proteomics. In a full‐face, double blind, vehicle‐controlled trial HSA was assessed for its effects on conspicuous facial pore size and degree of pigmentation of age spots in Caucasian women over an 8‐week period.ResultsHSA was obtained in enantiomeric pure, high yield (≥80%). Collagen type I and type III levels were dose‐dependently increased (96% and 244%; P < 0.01) in vitro and collagen type III in ex vivo skin by +57% (P < 0.01) by HSA. HSA also inhibited UVB‐induced MMP‐1 gene expression (83%; P < 0.01) and mitigated SBC induction (−34% vs. vehicle control) and reduced significantly UV‐induced p53 up‐regulation (−46% vs. vehicle control; P < 0.01) in irradiated skin. HSA modified the fibroblast secretome with significant increases in proteins associated with the WNT pathway that could reduce melanogenesis and proteins that could modify dermal fibroblast activity and keratinocyte differentiation to account for the alleviation of conspicuous pores. Docking studies in silico and EC50 determination in reporter gene assays (EC50 5.5 × 10−6 M) identified HSA as a peroxisomal proliferator activated receptor‐α (PPARα) agonist. Clinically, HSA showed a statistically significant decrease of surface and volume of skin pores (P < 0.05) after 8 weeks of application and age spots became significantly less pigmented than the surrounding skin (contrast, P < 0.05) after 4 weeks.ConclusionHSA acts as a PPARα agonist to reduce the signs of age spots and conspicuous pores by significantly modulating the expression of p53, SBC, MMP‐1 and collagen together with major changes in secreted proteins that modify keratinocyte, melanocyte and fibroblast cell behavior.

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Precise role of dermal fibroblasts on melanocyte pigmentation

Cited by 124 publications

References 89 publications

Characterization of human skin equivalents developed at body's core and surface temperatures

Characterization of human skin equivalents developed at body's core and surface temperatures

Melanoma in the Eyes of Mechanobiology

Bio‐derived hydroxystearic acid ameliorates skin age spots and conspicuous pores

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