Precise spatio‐temporal modulation of <scp>ACC</scp> synthase by <scp>MPK</scp>6 cascade mediates the response of rose flowers to rehydration

Meng, Yonglu; Ma, Nan; Zhang, Qian; You, Qi; Li, Na; Khan, Muhammad Ali; Liu, Xiaojing; Wu, Lin; Gao, Junping

doi:10.1111/tpj.12594

Cited by 35 publications

(29 citation statements)

References 66 publications

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“…Further research on rose flowers showed that, particularly in the gynoecia, protein levels of RhMPK6 are high during both dehydration and rehydration, but RhMPK6 kinase activity was observed only within the first hour of rehydration. Active RhMPK6 is able to phosphorylate and stabilize RhACS1, stimulating ET production ( Meng et al, 2014 ). The RhMPK6-RhACS1 module seems to be crucial for transduction of the rehydration signal and triggering of ET biosynthesis, which controls flower opening and senescence in rose.…”

Section: Mapk Kinase Cascades In Ethylene Biosynthesis and Signalingmentioning

confidence: 99%

Mitogen-Activated Protein Kinase Cascades in Plant Hormone Signaling

et al. 2018

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Mitogen-activated protein kinase (MAPK) modules play key roles in the transduction of environmental and developmental signals through phosphorylation of downstream signaling targets, including other kinases, enzymes, cytoskeletal proteins or transcription factors, in all eukaryotic cells. A typical MAPK cascade consists of at least three sequentially acting serine/threonine kinases, a MAP kinase kinase kinase (MAPKKK), a MAP kinase kinase (MAPKK) and finally, the MAP kinase (MAPK) itself, with each phosphorylating, and hence activating, the next kinase in the cascade. Recent advances in our understanding of hormone signaling pathways have led to the discovery of new regulatory systems. In particular, this research has revealed the emerging role of crosstalk between the protein components of various signaling pathways and the involvement of this crosstalk in multiple cellular processes. Here we provide an overview of current models and mechanisms of hormone signaling with a special emphasis on the role of MAPKs in cell signaling networks.One-sentence summary: In this review we highlight the mechanisms of crosstalk between MAPK cascades and plant hormone signaling pathways and summarize recent findings on MAPK regulation and function in various cellular processes.

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Section: Mapk Kinase Cascades In Ethylene Biosynthesis and Signalingmentioning

confidence: 99%

Mitogen-Activated Protein Kinase Cascades in Plant Hormone Signaling

et al. 2018

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“…The transcriptional and post-translational regulation of ethylene synthesis proved pivotal for plant development and ripening [ 34 , 52 – 54 ]. Previous studies demonstrated that wound- and ABA-induced ethylene synthesis mostly depended on post-transcriptional regulation [ 7 , 38 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

Oligogalacturonic acids promote tomato fruit ripening through the regulation of 1-aminocyclopropane-1-carboxylic acid synthesis at the transcriptional and post-translational levels

Zhou

Wang

et al. 2016

BMC Plant Biol

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BackgroundOligogalacturonic acids (OGs) are oligomers of alpha-1,4-linked galacturonosyl residues that are released from cell walls by the hydrolysis of polygalacturonic acids upon fruit ripening and under abiotic/biotic stress. OGs may induce ethylene production and fruit ripening, however, the mechanism(s) behind these processes is unknown.ResultsTomato cultivar ‘Ailsa Craig’ (AC) and mutant Neverripe, ripening inhibitor, non-ripening, and colorless non-ripening fruits were treated with OGs at different stages. Only AC fruits at mature green stage 1 showed an advanced ripening phenomenon, although transient ethylene production was detected in all of the tomato fruits. Ethylene synthesis genes LeACS2 and LeACO1 were rapidly up-regulated, and the phosphorylated LeACS2 protein was detected after OGs treatment. Protein kinase/phosphatase inhibitors significantly affected the ripening process induced by the OGs. As a potential receptor of OGs, LeWAKL2 was also up-regulated in their presence.ConclusionsWe demonstrated that OGs promoted tomato fruit ripening by inducing ethylene synthesis through the regulation of LeACS2 at transcriptional and post-translational levels.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-015-0634-y) contains supplementary material, which is available to authorized users.

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“…During dehydration and rehydration, the protein level of a specific MAP kinase, RhMPK6, was maintained at a constant high level. However, RhMPK6 activity was only detected within 1 h of rehydration [ 16 ]. Furthermore, activated RhMPK6 phosphorylated and stabilised RhACS1, a rate-limiting enzyme of ethylene biosynthesis, and resulted in an ethylene burst in gynoecia.…”

Section: Introductionmentioning

confidence: 99%

“…Previously, we found that a MAPKK protein, RhMKK9, could phosphorylate the RhMPK6 protein in vitro [ 16 ]. However, to date it is unknown whether RhMKK9 is the actual upstream component activating RhMPK6 in gynoecia during rehydration.…”

Section: Introductionmentioning

confidence: 99%

RhMKK9, a rose MAP KINASE KINASE gene, is involved in rehydration-triggered ethylene production in rose gynoecia

et al. 2017

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BackgroundFlower opening is an important process in the life cycle of flowering plants and is influenced by various endogenous and environmental factors. Our previous work demonstrated that rose (Rosa hybrida) flowers are highly sensitive to dehydration during flower opening and the water recovery process after dehydration induced ethylene production rapidly in flower gynoecia. In addition, this temporal- and spatial-specific ethylene production is attributed to a transient but robust activation of the rose MAP KINASE6-ACC SYNTHASE1 (RhMPK6-RhACS1) cascade in gynoecia. However, the upstream component of RhMPK6-RhACS1 is unknown, although RhMKK9 (MAP KINASE KINASE9), a rose homologue of Arabidopsis MKK9, could activate RhMPK6 in vitro. In this study, we monitored RhMKK2/4/5/9 expression, the potential upstream kinase to RhMPK6, in rose gynoecia during dehydration and rehydration.ResultsWe found only RhMKK9 was rapidly and strongly induced by rehydration. Silencing of RhMKK9 significantly decreased rehydration-triggered ethylene production. Consistently, the expression of several ethylene-responsive genes was down regulated in the petals of RhMKK9-silenced flowers. Moreover, we detected the DNA methylation level in the promoter and gene body of RhMKK9 by Chop-PCR. The results showed that rehydration specifically elevated the DNA methylation level on the RhMKK9 gene body, whereas it resulted in hypomethylation in its promoter.ConclusionsOur results showed that RhMKK9 possibly acts as the upstream component of the RhMKK9-RhMPK6-RhACS1 cascade and is responsible for water recovery-triggered ethylene production in rose gynoecia, and epigenetic DNA methylation is involved in the regulation of RhMKK9 expression by rehydration.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-017-0999-1) contains supplementary material, which is available to authorized users.

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Precise spatio‐temporal modulation of ACC synthase by MPK6 cascade mediates the response of rose flowers to rehydration

Cited by 35 publications

References 66 publications

Mitogen-Activated Protein Kinase Cascades in Plant Hormone Signaling

Mitogen-Activated Protein Kinase Cascades in Plant Hormone Signaling

Oligogalacturonic acids promote tomato fruit ripening through the regulation of 1-aminocyclopropane-1-carboxylic acid synthesis at the transcriptional and post-translational levels

RhMKK9, a rose MAP KINASE KINASE gene, is involved in rehydration-triggered ethylene production in rose gynoecia

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