Precisely Controlled Delivery of Abaloparatide through Injectable Hydrogel to Promote Bone Regeneration

Ning, Ziyu; Tan, Baoyu; Chen, Bin; Lau, Dzi Shing Aaron; Wong, TM; Sun, Tianhao; Peng, Songlin; Li, Zhaoyang; Lu, William W.

doi:10.1002/mabi.201900020

Cited by 32 publications

(22 citation statements)

References 33 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SEM indicated that GelMA and GA/GelMA are porous, which contributes to osteogenesis. Ning et al (Ning et al, 2019) reported that injectable photo-crosslinked GelMA hydrogel promoted the healing of bone defects in rats. However, pure GelMA hydrogel is difficult to greatly promote bone regeneration in the absence of any bioactive agent or pretreatment (mineralization in vitro), limiting its application in bone repairing (Zheng et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Glycyrrhizic Acid Promotes Osteogenic Differentiation of Human Bone Marrow Stromal Cells by Activating the Wnt/β-Catenin Signaling Pathway

Bai

Hang

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

Glycyrrhizic acid (GA) is a major triterpene glycoside isolated from liquorice root that has been shown to inhibit osteoclastogenesis. However, there have been no reports regarding the effect of GA on osteogenic differentiation. Therefore, this study was performed to explore the effects and mechanism of action of GA on osteogenesis. A CCK-8 array was used to assess cell viability. The osteogenic capability was investigated by real-time quantitative PCR, western blotting and immunofluorescence analyses. ALP staining and ARS were used to evaluate ALP activity and mineralization, respectively. GA-GelMA hydrogels were designed to verify the therapeutic effects of GA in vivo by radiographic analysis and histological evaluation. Our results show that GA had no significant influence on the viability or proliferation of human bone marrow stromal cells (hBMSCs). GA promoted osteogenic differentiation and enhanced calcium deposition. Furthermore, ratio of active β-catenin and total β-catenin protein increased after treatment with GA. Wnt/catenin signaling inhibitor partially attenuated the effects of GA on osteogenic differentiation. In a mouse femoral fracture model, GA-GelMA hydrogels accelerated bone healing. Our results show that GA promotes the osteogenic differentiation of hBMSCs by modulating the Wnt/β-catenin signaling pathway. GA-GelMA hydrogels promoted bone fracture healing. GA has potential as a cost-effective treatment of bone defects.

show abstract

Section: Discussionmentioning

confidence: 99%

Glycyrrhizic Acid Promotes Osteogenic Differentiation of Human Bone Marrow Stromal Cells by Activating the Wnt/β-Catenin Signaling Pathway

Bai

Hang

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…SEM indicated that GelMA and GA/GelMA are porous, which contributes to osteogenesis. Ning et al (Ning et al, 2019) reported that injectable photo-crosslinked GelMA hydrogel promoted the healing of bone defects in rats. We designed a GA-delivered GelMA hydrogel, which was used in a mouse femoral fracture model, and the results of micro-CT and histological evaluation indicated that it had a superior effect on bone-fracture healing.…”

Section: Discussionmentioning

confidence: 99%

Glycyrrhizic acid promotes osteogenic differentiation of human bone mesenchymal stem cells by activating the Wnt/β-catenin signalling pathway

Xue

Bai

et al. 2020

Preprint

View full text Add to dashboard Cite

Background and Purpose Glycyrrhizic acid (GA) is a major triterpene glycoside isolated from liquorice root that has been shown to inhibit osteoclastogenesis. However, there have been no reports regarding the effect of GA on osteogenic differentiation. Therefore, this study was performed to explore the effects and mechanism of action of GA on osteogenesis. Experimental Approach A CCK-8 array was used to assess cell viability. The osteogenic capability was investigated by real-time quantitative PCR, western blotting and immunofluorescence analyses. ALP staining and ARS were used to evaluate ALP activity and mineralisation, respectively. GA-GelMA hydrogels were designed to verify the therapeutic effects of GA in vivo by radiographic analysis and histological evaluation. Key Results Our results show that GA had no significant influence on the viability or proliferation of human bone mesenchymal stem cells (hBMSCs). GA promoted osteogenic differentiation and enhanced calcium deposition. Furthermore, levels of active β-catenin protein increased after treatment w ith GA. Wnt/catenin signalling inhibitor partially reversed the effects of GA on osteogenic differentiation. In a mouse femoral fracture model, GA-GelMA hydrogels accelerated bone healing. Conclusion and Implications Our results show that GA promotes the osteogenic differentiation of hBMSCs by modulating the Wnt/β-catenin signalling pathway. GA-GelMA hydrogels promoted bone fracture healing. GA has potential as a cost-effective treatment of bone defects. Title:Glycyrrhizic acid promotes osteogenic differentiation of human bone mesenchymal stem cells by activating the Wnt/β-catenin signalling pathway Running title: Glycyrrhizic acid promotes osteogenesis through Wnt/β-catenin pathway

show abstract

“…Covalent conjugation/ physical absorption [ 167,168] TRSAW (PTHrP107-111) -Covalent conjugation [ 169] RKVRGPPVSCIKRDSPIQ (LP2) -Covalent conjugation/ physical absorption [ 170] DGEA…”

Section: Angiogenesismentioning

confidence: 99%

Supramolecular Peptide Nanofiber Hydrogels for Bone Tissue Engineering: From Multihierarchical Fabrications to Comprehensive Applications

Hao

Wang

et al. 2022

Advanced Science

View full text Add to dashboard Cite

Bone tissue engineering is becoming an ideal strategy to replace autologous bone grafts for surgical bone repair, but the multihierarchical complexity of natural bone is still difficult to emulate due to the lack of suitable biomaterials. Supramolecular peptide nanofiber hydrogels (SPNHs) are emerging biomaterials because of their inherent biocompatibility, satisfied biodegradability, high purity, facile functionalization, and tunable mechanical properties. This review initially focuses on the multihierarchical fabrications by SPNHs to emulate natural bony extracellular matrix. Structurally, supramolecular peptides based on distinctive building blocks can assemble into nanofiber hydrogels, which can be used as nanomorphology-mimetic scaffolds for tissue engineering. Biochemically, bioactive motifs and bioactive factors can be covalently tethered or physically absorbed to SPNHs to endow various functions depending on physiological and pharmacological requirements. Mechanically, four strategies are summarized to optimize the biophysical microenvironment of SPNHs for bone regeneration. Furthermore, comprehensive applications about SPNHs for bone tissue engineering are reviewed. The biomaterials can be directly used in the form of injectable hydrogels or composite nanoscaffolds, or they can be used to construct engineered bone grafts by bioprinting or bioreactors. Finally, continuing challenges and outlook are discussed.

show abstract

Precisely Controlled Delivery of Abaloparatide through Injectable Hydrogel to Promote Bone Regeneration

Cited by 32 publications

References 33 publications

Glycyrrhizic Acid Promotes Osteogenic Differentiation of Human Bone Marrow Stromal Cells by Activating the Wnt/β-Catenin Signaling Pathway

Glycyrrhizic Acid Promotes Osteogenic Differentiation of Human Bone Marrow Stromal Cells by Activating the Wnt/β-Catenin Signaling Pathway

Glycyrrhizic acid promotes osteogenic differentiation of human bone mesenchymal stem cells by activating the Wnt/β-catenin signalling pathway

Supramolecular Peptide Nanofiber Hydrogels for Bone Tissue Engineering: From Multihierarchical Fabrications to Comprehensive Applications

Contact Info

Product

Resources

About