Precisely mapping a major gene conferring resistance to Hessian fly in bread wheat using genotyping-by-sequencing

Abstract

BackgroundOne of the reasons hard red winter wheat cultivar ‘Duster’ (PI 644016) is widely grown in the southern Great Plains is that it confers a consistently high level of resistance to biotype GP of Hessian fly (Hf). However, little is known about the genetic mechanism underlying Hf resistance in Duster. This study aimed to unravel complex structures of the Hf region on chromosome 1AS in wheat by using genotyping-by-sequencing (GBS) markers and single nucleotide polymorphism (SNP) markers.ResultsDoubled haploid (DH) lines generated from a cross between two winter wheat cultivars, ‘Duster’ and ‘Billings’ , were used to identify genes in Duster responsible for effective and consistent resistance to Hf. Segregation in reaction of the 282 DH lines to Hf biotype GP fit a one-gene model. The DH population was genotyped using 2,358 markers developed using the GBS approach. A major QTL, explaining 88% of the total phenotypic variation, was mapped to a chromosome region that spanned 178 cM and contained 205 GBS markers plus 1 SSR marker and 1 gene marker, with 0.86 cM per marker in genetic distance. The analyses of GBS marker sequences and further mapping of SSR and gene markers enabled location of the QTL-containing linkage group on the short arm of chromosome 1A. Comparative mapping of the common markers for the gene for QHf.osu-1Ad in Duster and the Hf-resistance gene for QHf.osu-1A74 in cultivar ‘2174’ showed that the two Hf resistance genes are located on the same chromosome arm 1AS, only 11.2 cM apart in genetic distance. The gene at QHf.osu-1Ad in Duster has been delimited within a 2.7 cM region.ConclusionTwo distinct resistance genes exist on the short arm of chromosome 1A as found in the two hard red winter cultivars, 2174 and Duster. Whereas the Hf resistance gene in 2174 is likely allelic to one or more of the previously mapped resistance genes (H9, H10, H11, H16, or H17) in wheat, the gene in Duster is novel and confers a more consistent phenotype than 2174 in response to biotype GP infestation in controlled-environment assays.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1297-7) contains supplementary material, which is available to authorized users.

Development and Validation of KASP Markers for Wheat Streak Mosaic Virus Resistance Gene Wsm2

“…Recent advances in high‐throughput genotyping technology have enabled the discovery of thousands of molecular markers in wheat, mainly single‐nucleotide polymorphisms (SNPs). The development of high‐density SNP markers in hexaploid wheat are widely used to detect the association between markers and traits in genetic mapping experiments (Cabral et al, 2014; Forrest et al, 2014; Jighly et al, 2015; Li et al, 2015; Assanga et al, 2016). A recent study used the 90K Infinium iSelect SNP array to generate a high‐resolution genetic map for identification of SNPs tightly linked to Wsm2 in a recombinant inbred line (RIL) population derived from a cross of CO960293–2 × ‘TAM 111’ (Assanga et al, 2016).…”

Genetic characterization and deployment of a major gene for grain yield on chromosome arm 1BS in winter wheat

“…In a previous study (Li et al 2015), a doubled haploid (DH) population was developed from two locally adapted winter wheat cultivars, "Duster" (PI 644016) and "Billings" (PI 656843), both of which are released by the Oklahoma Agricultural Experiment Station. Duster and Billings have occupied significant acreages in the Southern Great Plains due to their outstanding yielding ability (Edwards et al 2012;Hunger et al 2014).…”

“…The Duster × Billings DH population was genotyped using genotyping-by-sequencing (GBS) approach, and a total of 2358 GBS markers were subjected to linkage mapping for the DH lines (Li et al 2015). The GBS marker sequences were used to blast IWGSC RefSeq v1.0 (https://urgi.versailles.inra.fr/blast/?dbgroup= wheat_iwgsc_refseq_v1_chromosomes), and the resulting physical locations were ordered along each chromosome.…”

Avirulence gene mapping in the Hessian fly (Mayetiola destructor) reveals a protein phosphatase 2C effector gene family

“…2). By convention, the chromosomes composing a HF zygote"s genome are grouped into eight "S" and a variable number (28)(29)(30)(31)(32) of "E" chromosomes (Stuart et al, 2012). The S chromosomes are divided further into two autosome pairs (A1 and A2) and two "X" chromosome pairs (X1 and X2).…”