Precision assessment of label-free psoriasis biomarkers with ultra-broadband optoacoustic mesoscopy

Aguirre, Juan; Schwarz, Michael; Garzorz, Natalie; Omar, Murad; Buehler, Andreas; Eyerich, Kilian; Ntziachristos, Vasilis

doi:10.1038/s41551-017-0068

Cited by 238 publications

(336 citation statements)

References 27 publications

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“…Therefore, even larger changes in this small volume are not detectable. Aguirre et al [40] could demonstrate the effect of psoriasis on dermal microvasculature by using raster scan photoacoustic mesoscopy in an ultra-broadband mode (10-180 MHz), which achieves a higher level of detail and resolution-to-depth ratio.…”

Section: Discussionmentioning

confidence: 99%

“…As the resolution of the photoacoustic system applied was not high enough to represent the changes in the structure of the capillary loops, the performed photoacoustic measurements are unsuitable for the described investigations. To make it suitable, the frequency of the ultrasonic transducer would have to be increased, so that a higher resolution can be achieved [40]. Consequently, laser scanning microscopy remains the method of choice for the detection of pathological changes in psoriatic skin.…”

Section: Discussionmentioning

confidence: 99%

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Application of Photoacoustic Methods and Confocal Microscopy for Monitoring of Therapeutic Response in Plaque Psoriasis

Ossadnik

Philipp

Bost

et al. 2018

Skin Pharmacol Physiol

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Psoriasis is prone to relapses and requires long-term therapy that may induce a range of adverse effects; therefore, an efficient and early detection of relapses is desirable. In this study, photoacoustic imaging and confocal laser scanning microscopic (CLSM) methods were investigated for their suitability in psoriasis follow-up examinations. Using a high-resolution photoacoustic system, the vascular structures of 11 psoriatic patients and 6 healthy volunteers were investigated. No differences were detected with respect to the average vessel diameter and vasculature per unit volume in the tissue of healthy volunteers and non-lesional and lesional skin areas of psoriatic patients. By means of CLSM, the diameters of the dermal papillae of 6 volunteers and 6 psoriatic patients were determined. The diameters of the dermal papillae of the healthy volunteers (0.074 ± 0.006 mm) revealed no significant difference when compared to non-lesional skin areas of psoriatic patients (0.079 ± 0.005 mm). The results obtained for the lesions in psoriatic patients showed a significant difference (Wilcoxon test, p = 0.028) between the diameters of the dermal papillae of the lesional skin areas (0.114 ± 0.012 mm) and the non-lesional skin areas (0.079 ± 0.005 mm). Thus, CLSM can be applied for monitoring psoriasis follow-up examinations.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Application of Photoacoustic Methods and Confocal Microscopy for Monitoring of Therapeutic Response in Plaque Psoriasis

Ossadnik

Philipp

Bost

et al. 2018

Skin Pharmacol Physiol

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“…These real-time, whole-body PAT systems are extremely powerful when tracking exogenously labeled drug molecules in pharmacokinetic studies on small animal models, thereby enabling biomedical researchers to test new drugs and monitor longitudinal therapy in the future. (2) The spatial resolutions of PAT have been pushing the existing limits through the use of high-frequency wideband ultrasound detection [22, 58]. Agurirre et al recently reported an ultra-broadband PAM system for human skin imaging with a spatial resolution of 7 μm, enabled by an ultrasonic detection band of 10–180 MHz [22].…”

Section: Advances In Pat Implementationsmentioning

confidence: 99%

“…(2) The spatial resolutions of PAT have been pushing the existing limits through the use of high-frequency wideband ultrasound detection [22, 58]. Agurirre et al recently reported an ultra-broadband PAM system for human skin imaging with a spatial resolution of 7 μm, enabled by an ultrasonic detection band of 10–180 MHz [22]. Guggenheim et al .…”

Section: Advances In Pat Implementationsmentioning

confidence: 99%

Recent progress in photoacoustic molecular imaging

Yao

Wang

2018

Current Opinion in Chemical Biology

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By acoustically detecting the optical absorption contrast, photoacoustic (PA) tomography (PAT) has broken the penetration limits of traditional high-resolution optical imaging. Through spectroscopic analysis of the target's optical absorption, PAT can identify a wealth of endogenous and exogenous molecules and thus is inherently capable of molecular imaging with high sensitivity. PAT's molecular sensitivity is uniquely accompanied by non-ionizing radiation, high spatial resolution, and deep penetration in biological tissues, which other optical imaging modalities cannot achieve yet. In this concise review, we summarize the most recent technological advancements in PA molecular imaging and highlight the novel molecular probes specifically made for PAT in deep tissues. We conclude with a brief discussion of the opportunities for future advancements.

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“…PAI and US signals, for example, are detectable using the same equipment, making this pair attractive, as illustrated by a handheld hybrid US-MSOT probe for the visualisation of sentinel lymph nodes and the determination of their metastatic status in melanoma; in a 20-patient clinical trial 149 , indocyanine green (ICG) and melanin contrast were detected up to 5 cm in depth, and delivered 100% sensitivity and 48.6% specificity for metastatic status, as verified by histology. A similar US-MSOT system demonstrated simultaneous imaging of haemoglobin oxygen saturation and flow (Doppler US) in the vasculature of healthy human volunteers 150 , and frequency splitting in ultra-broadband optoacoustics identified inflammation parameters for the staging of psoriasis 151 . However, most state-of-the-art MSOT systems are still in prototypical design phase, which is a hurdle to regulatory approval and clinical adoption.…”

Section: Combining Data From Different Molecular Contrastsmentioning

confidence: 99%

Multiplexed imaging for diagnosis and therapy

et al. 2017

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Complex molecular and metabolic phenotypes depict cancers as a constellation of different diseases with common themes. Precision imaging of such phenotypes requires flexible and tuneable modalities capable of identifying phenotypic fingerprints by using a restricted number of parameters whilst ensuring sensitivity to dynamic biological regulation. Common phenotypes can be detected by in vivo imaging technologies, and effectively define the emerging standards for disease classification and patient stratification in radiology. However, for the imaging data to accurately represent a complex fingerprint, the individual imaging parameters need to be measured and analysed in relation to their wider spatial and molecular context. In this respect, targeted palettes of molecular imaging probes facilitate the detection of heterogeneity in oncogene-driven alterations and their response to treatment, and lead to the expansion of rational-design elements for the combination of imaging experiments. In this Review, we evaluate criteria for conducting multiplexed imaging, and discuss its opportunities for improving patient diagnosis and the monitoring of therapy.

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Precision assessment of label-free psoriasis biomarkers with ultra-broadband optoacoustic mesoscopy

Cited by 238 publications

References 27 publications

Application of Photoacoustic Methods and Confocal Microscopy for Monitoring of Therapeutic Response in Plaque Psoriasis

Application of Photoacoustic Methods and Confocal Microscopy for Monitoring of Therapeutic Response in Plaque Psoriasis

Recent progress in photoacoustic molecular imaging

Multiplexed imaging for diagnosis and therapy

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