Precision engineering for localization, validation, and modification of allergenic epitopes

Pomés, Anna; Smith, Scott A.; Chruszcz, Maksymilian; Mueller, Geoffrey A.; Brackett, Nicole F.; Chapman, Martin D.

doi:10.1016/j.jaci.2023.12.017

Cited by 6 publications

(1 citation statement)

References 126 publications

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“…Advanced bioinformatic tools different from those defined by Codex ( 10 ), the classical FASTA algorithm, are for example similarity searches of 3D protein structure ( 11 , 12 ), machine learning based on mapping of IgE epitope and motif search ( 13 ) or new approaches considering allergen-IgE interaction ( 14 ). It is highly likely that these advanced bioinformatic tools will provide higher sensitivity, specificity, accuracy, and improve allergenicity prediction.…”

Section: Allergenicity Prediction In Risk Assessment—current State An...mentioning

confidence: 99%

Research gaps and future needs for allergen prediction in food safety

Fernandez,

Danisman,

Taheri Boroujerdi

et al. 2024

Front. Allergy

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The allergenicity and protein risk assessments in food safety are facing new challenges. Demands for healthier and more sustainable food systems have led to significant advances in biotechnology, the development of more complex foods, and the search for alternative protein sources. All this has increased the pressure on the safety assessment prediction approaches anchored into requirements defined in the late 90's. In 2022, the EFSA's Panel on Genetically Modified Organisms published a scientific opinion focusing on the developments needed for allergenicity and protein safety assessments of new products derived from biotechnology. Here, we further elaborate on the main elements described in this scientific opinion and prioritize those development needs requiring critical attention. The starting point of any new recommendation would require a focus on clinical relevance and the development of a fit-for-purpose database targeted for specific risk assessment goals. Furthermore, it is imperative to review and clarify the main purpose of the allergenicity risk assessment. An internationally agreed consensus on the overall purpose of allergenicity risk assessment will accelerate the development of fit-for-purpose methodologies, where the role of exposure should be better clarified. Considering the experience gained over the last 25 years and recent scientific developments in the fields of biotechnology, allergy, and risk assessment, it is time to revise and improve the allergenicity safety assessment to ensure the reliability of allergenicity assessments for food of the future.

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Section: Allergenicity Prediction In Risk Assessment—current State An...mentioning

confidence: 99%

Research gaps and future needs for allergen prediction in food safety

Fernandez,

Danisman,

Taheri Boroujerdi

et al. 2024

Front. Allergy

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The Potential of Human Monoclonal IgE Antibodies to Establish Biological Potency and Stability of Allergen Extracts

Rabin

2024

Curr Allergy Asthma Rep

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Purpose of Review Allergenic extracts are often standardized to control for potency, either by measuring concentrations of major allergens or “overall allergenicity” by competition for IgE in pooled sera from highly allergic subjects with a reference extract. Recent developments present an opportunity to use human mAb cloned from highly allergic subjects to define potency of allergenic extracts. Recent Findings Two recent developments present an opportunity for revising potency measurements of allergen extracts: cloning allergen specific IgE from allergic subjects and extensive epitope mapping of major allergenic proteins. Summary Because human IgE mAb recognize biologically relevant epitopes, they present a novel opportunity to determine the potencies of allergenic extracts and may contribute to the science base for allergen standardization.

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