Precision measurements of dimensions, shape and mass density of spermatozoan heads in normal and subfertile human males

Duijn, C. Van; Voorst, C. van; Hellinga, G

doi:10.1016/0028-2243(72)90064-0

Cited by 15 publications

(10 citation statements)

References 12 publications

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“…Morphological damage of the tail and collapse of the neck piece caused by the shock waves were observed under electron microscopy. It is generally thought that the junction between the head and tail of the human sperma tozoa is most delicate [16], However, our experiments revealed that shock waves not only result in damage to the junction between the sperm head and tail, but also to the tail itself. Rüdiger et al [4] reported the histomorphological examination of rat testicles after 24 h and 3 months of exposure with 2,000 shock waves using a Wolf Piezolith 2200 lithotriptor.…”

Section: Discussionmentioning

confidence: 40%

Effects of Shock Waves on the Male Reproductive System

et al. 1993

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Although many reports concerning the effects of shock waves on the human body have been published, their effects on the male reproductive system are not well known. This report examines the effects of shock waves on human spermatozoa and rat testes. Semen samples were placed in water at 37 °C and 100, 200, 500, and 1,000 shock waves were applied. In proportion to an increasing number of shock waves, sperm motility and the percentage of viable sperm decreased, while the percentage of short-tailed sperm increased. In a second study 500 or 1,000 shock waves were applied to the testes of infant and adult male Wistar rats. Hematomas were observed macroscopically in the testes immediately after shock wave treatment. Light microscopy showed rupture of the basement membrane of the seminiferous tubules and outflow of spermatogenic cells. After 5 weeks, the treated testes appeared atrophic, with the absence of spermatogenic cells in the seminiferous tubules, except for Sertoli cells. Our data indicate that spermatozoa and the testis appear to be structurally damaged by shock waves.

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Section: Discussionmentioning

confidence: 40%

Effects of Shock Waves on the Male Reproductive System

et al. 1993

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“…It therefore appears that head dimensions may not be exclusively a result of aberrant P1/P2 ratios and may involve other unidentified defects during spermatid maturation, van Duijn et al (34) and Schmassman et al (20) showed sperm head dimensions to be higher in subfertile patients than in fertile patients, although these studies did not look at sperm protamine levels. The head dimensions reported by van Duijn et al (34) were similar to our findings. Thompson et al (28) also studied sperm head dimensions and showed that sperm that bind to the zona pellucida have different head dimensions than sperm that do not.…”

Section: Discussionmentioning

confidence: 98%

Human protamines and male infertility

Khara

Vlad

Griffiths

et al. 1997

J Assist Reprod Genet

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“…On the other hand, various authors have also attempted to relate male fertility to the sperm population distribution in an individual [van Duijn, 1975]. Although van Duijn [1975] reported that a skewed sperm population distribution is not necessarily related to low male fertility, the coefficient of variation of the biometric parameters of size and shape tends to be greater in humans with fertility problems [van Duijn et al, 1972]. No spermatic subpopulations were apparent in our study, as the distributions of the measurements taken were relatively homogeneous.…”

Section: Discussionmentioning

confidence: 99%

Use of computerized image analysis in the morphometric characterization of giant panda (Ailuropoda melanoleuca) spermatozoa obtained from the epididymis 4 hours postmortem

Pérez-Garnelo

Delclaux²,

Talavera³

et al. 2003

Zoo Biology

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The reproductive tract of Chu-Lin, a giant panda that had died at Madrid Zoo, was removed. Four hours postmortem the epididymides was cut with a blade, and the spermatozoa were collected and immersed in a freezing medium. The sample was kept under refrigeration at 51C. After 4 hr the sperm suspension was aspirated into 0.25-mL straws, which were held for 7 min in nitrogen vapors before they were plunged into liquid nitrogen. To characterize sperm population and distribution, a morphometric study of the triple-stained smears of the thawed semen sample was undertaken with the aid of computerized image analysis. Spermatozoa with intact acrosomes were selected and assessed using an immersion lens (1,000 Â ) and bright-field illumination. The software made it possible to take six basic linear measurements of each spermatozoon: maximum head width (HW), base of the head (BH), maximum head length (HL), midpiece length (ML), midpiece width (MW), and terminal piece length (TL). The system was connected to a data base from which derived parameters could be calculated: head form factor (HL/HW), total flagellum length (TFL), total sperm length (TSL), and the proportion of each of the sections in relation to the whole. The results of the morphometric study did not demonstrate the existence of sperm subpopulations, as the measurements of the 100 spermatozoa analyzed were relatively homogeneous with regard to their distribution for each of the parameters considered. The sperm head of the giant panda displayed a relatively rounded morphology, and the HL/HW was 1.2470.03. Mean head length was

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Precision measurements of dimensions, shape and mass density of spermatozoan heads in normal and subfertile human males

Cited by 15 publications

References 12 publications

Effects of Shock Waves on the Male Reproductive System

Effects of Shock Waves on the Male Reproductive System

Human protamines and male infertility

Use of computerized image analysis in the morphometric characterization of giant panda (Ailuropoda melanoleuca) spermatozoa obtained from the epididymis 4 hours postmortem

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