Precision targeted ruthenium(<scp>ii</scp>) luminophores; highly effective probes for cell imaging by stimulated emission depletion (STED) microscopy

Byrne, Aisling; Burke, Christopher S.; Keyes, Tia E.

doi:10.1039/c6sc02588a

Cited by 73 publications

(92 citation statements)

References 63 publications

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“…, the capability of light-induced switching between ‘on’ and ‘off’ states. 19 Many conventional fluorophores including organic dyes and fluorescent proteins have been utilized for live-cell nanoscopy, 15,20,21 whereas there are only very few reports employing nanoparticles for this purpose. 22–24 Development of NPs for super-resolution imaging (sriNPs) can greatly enrich the toolbox of robust optical nanoprobes for biological studies.…”

Section: Introductionmentioning

confidence: 99%

Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells

Gao

Wang

et al. 2017

Chem. Sci.

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Section: Introductionmentioning

confidence: 99%

Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells

Gao

Wang

et al. 2017

Chem. Sci.

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“…Suitable STED probes can be used as cargos for selective visualisation of organelles. In this sense, ruthenium polypyridyl complexes have been conjugated with targeted and untargeted peptide sequences (nuclear localisation sequences, endoplasmic reticulum directing sequences, untargeted octa‐arginine sequences), thus allowing high‐resolution imaging of the endoplasmic reticulum and the cell nucleus …”

Section: Othersmentioning

confidence: 99%

“…In this sense, ruthenium polypyridyl complexes have been conjugated with targeted and untargeted peptides equences (nuclear localisation sequences,e ndoplasmic reticulumd irecting sequences, untargeted octa-arginines equences), thus allowing highresolution imaging of the endoplasmicr eticulum and the cell nucleus. [112] Another technique for high-resolution studies is correlative light and electron microscopy (CLEM), [113] in which samples of live cells are examined by fluorescence microscopy and then processed for electron microscopy. [113] Thed evelopment of the technique and related equipment has provided the potential to acquire both images almosts imultaneouslyi nanew gener-ation of integrated CLEM microscopes.…”

Section: Othersmentioning

confidence: 99%

Where in the Cell Is our Cargo? Methods Currently Used To Study Intracellular Cytosolic Localisation

2018

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The internalisation and delivery of active substances into cells is a field of growing interest for chemical biology and therapeutics. As we move from small-molecule-based drugs towards bigger cargos, such as antibodies, enzymes, nucleases or nucleic acids, the development of efficient delivery systems becomes critical for their practical application. Different strategies and synthetic carriers have been developed; these include cationic lipids, gold nanoparticles, polymers, cell-penetrating peptides (CPPs), protein surface modification etc. However, all of these methodologies still present limitations relating to the precise targeting of the different intracellular compartments and, in particular, difficulties in access to the cellular cytosol. Additionally, the precise quantification of the cellular uptake of a compound is not enough to demonstrate delivery and/or functional activity. Therefore, methods to determine cellular distributions of cargos and carriers are of critical importance for identifying the barriers that are blocking the activity. Herein we survey the different techniques that can currently be used to track and to monitor the subcellular localisation of the synthetic compounds that we deliver inside cells.

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“…Fluorescence imaging as a feasible method has been widely used to visualize the activity of biological processes that influence the behavior of cancers or responsiveness to therapeutic drugs in a simple, convenient, and diversified way . However, conventional fluorescence imaging exhibits aggregation‐caused quenching effect which is not suitable for long‐term tracking in the cell nucleus . Fortunately, Tang's group observed an intriguing phenomenon named the aggregation‐induced emission (AIE) effect in 2001 .…”

Section: Introductionmentioning

confidence: 99%

Construction of Bovine Serum Albumin/AIE‐Based Quaternary Complexes for Efficient Gene Transfection

Liu

Pang

et al. 2018

Macromolecular Bioscience

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High transfection efficiency and superior cell imaging are required for cationic polymers‐based gene delivery system to afford high therapeutic effect but its high toxicity and unstable cell imaging are easily ignored. In this study, cationic amino poly(glycerol methacrylate) derivative (PGMA‐EDA) is used to incorporate bovine serum albumin (BSA) and aggregation‐induced emission (AIE) molecular (tetraphenylethylene derivatives, TPE) as an efficient carrier for gene transfection and intracellular imaging. The obtained polymer/pDNA‐TPE/BSA (PDTB) quaternary nanoparticles (NPs) not only exhibit efficient gene transfection but also show excellent biocompatibility. After inclusion of TPE/BSA (TB) NPs, BSA promoted dissociation of the complexes upon being protonated and the lipophilic TPE‐reduced endosomal membrane stability, which enhanced endosomal escape of pDNA payload, finally resulting in an excellent gene transfection. On the other hand, less positive surface charge of PDTB NPs than that of the binary PD complexes, as well as the addition of biocompatible BSA, both factors contribute to the improved cell viability. Moreover, the AIE feature of TPE compared to aggregation‐caused quenching character of conventional fluorophores enables the complex with stably tracking the delivery of pDNA into cancer cells. Therefore, the newly developed PDTB complexes may be a promising candidate vector for traceable, safe, and effective gene delivery.

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Precision targeted ruthenium(ii) luminophores; highly effective probes for cell imaging by stimulated emission depletion (STED) microscopy

Abstract: Using precision peptide targeting to discrete cell organelles, it is demonstrated that Ru(ii) polypyridyl complexes are highly effective probes for stimulated emission depletion microscopy.

Cited by 73 publications

References 63 publications

Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells

Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells

Where in the Cell Is our Cargo? Methods Currently Used To Study Intracellular Cytosolic Localisation

Construction of Bovine Serum Albumin/AIE‐Based Quaternary Complexes for Efficient Gene Transfection

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