Preclinical development of gastro-protective botanical candidate from Woodfordia fruticosa  Chemical standardization, efficacy, pharmacokinetics and safety pharmacology

Khan, Inshad Ali; Singh, Amarinder; Mindala, Durga Prasad; Meena, Samdarshi; Vij, Bhavana; Yadav, Arvind Kumar; Roy, Sumit; Nandi, Utpal; Katare, Anil Kumar; Jaglan, Sundeep; Singh, Deepika; Gupta, Ajai Prakash; Gupta, Prasoon; Singh, Surjeet; Tikko, Manoj K.; Singh, Gurdarshan; Vishwakarma, Ram A.

doi:10.1016/j.jep.2019.112023

Cited by 11 publications

(5 citation statements)

References 12 publications

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“…The antioxidant and antimicrobial activities of the extracts obtained in our research have been therefore evaluated to find possible applications of cereal by-products in the dermo-functional and cosmetic industries, as natural preservatives, but other exploitations in the food sector as fortification agents and antioxidant additives may be considered ( Galanakis, 2018 ). Starting to the fact that WHO or any regulatory bodies stress on the importance of qualitative and quantitative methods for characterizing botanicals (quantifying biomarkers and chemical markers and fingerprinting their profiles) with the aim of achieving the standardization of botanicals and guaranteeing a reproducible safety and efficacy ( Khan et al, 2019 ), we chemically characterized the obtained extracts with various approaches. Briefly, the main goals of the present study were to investigate the contribution of the sonication effect during chemical hydrolysis in order to apply a more sustainable and effective extraction method from durum wheat and rice by-products, compared to those commonly used, to standardize the obtained extracts and to propose a possible industrial application.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and antimicrobial extracts obtained from agricultural by-products: Strategies for a sustainable recovery and future perspectives

Guerrini

Burlini

Lorenzo

et al. 2020

Food and Bioproducts Processing

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Section: Introductionmentioning

confidence: 99%

Antioxidant and antimicrobial extracts obtained from agricultural by-products: Strategies for a sustainable recovery and future perspectives

Guerrini

Burlini

Lorenzo

et al. 2020

Food and Bioproducts Processing

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“…The pharmacokinetic study of MEF M2 was based on the estimation of the most stable marker in plasma [ 18 ]. Mahanine was found to be the most stable, major marker and was therefore chosen for pharmacokinetics studies.…”

Section: Resultsmentioning

confidence: 99%

Preclinical Development of Mahanine-Enriched Fraction from Indian Spice Murraya koenigii for the Management of Cancer: Efficacy, Temperature/pH stability, Pharmacokinetics, Acute and Chronic Toxicity (14-180 Days) Studies

Satyavarapu

Sinha

Mandal

2020

BioMed Research International

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Murraya koenigii is well documented in the Indian ancient medical text “Charaka Samhita.” The carbazole alkaloid “mahanine” from this plant exhibited anticancer activity against several cancers. Here, we have taken a comprehensive study to standardize the method for the preparation of a mahanine-enriched fraction (MEF) with the highest yield and defined markers. Our optimized method produced MEF having the highest amount of mahanine, a major marker, with excellent in vitro antiproliferative activity against ovarian and breast cancer cells as evidenced by decreased cell viability by MTT assay. Moreover, it exhibited condensed and fragmented nuclei by DAPI staining and increased annexin V-/PI-stained cells after MEF treatment, indicating apoptosis. It also exhibited good efficacy in ovarian and breast cancer syngeneic mice models, with an ED50 of 300 mg/kg body weight (BW). MEF is stable up to 40°C for ≥3 months. Its biological activity remains unchanged at a wide range of pH (1-10) for up to ~3 hours, indicating a safe oral route of administration. Additionally, the comparative pharmacokinetics of MEF and mahanine in rats showed a 31% higher bioavailability of mahanine in MEF-fed rats compared to rats fed with mahanine alone. Furthermore, mice fed with MEF at 5000 mg/kg BW single dose, 300-1500 mg/kg BW/day for 14 days, and 300 mg/kg BW/day for 28, 90, and 180 days for subacute, subchronic, chronic studies, respectively, did not show any significant clinical signs of toxicity, behavioral changes, mortality, organ weights, serum biochemistry, and hematological parameters indicating no/minimum toxicity for up to 180 days. To the best of our knowledge, this is the first report showing the pH/temperature stability and chronic toxicity studies of MEF along with in vivo efficacy against breast cancer. Taken together, our study will enhance the commercial value of this highly potential medicinal plant and will be helpful as a reference material for its clinical development.

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“…The main phytochemicals found in the W. fruticosa are tannins, polyphenolic groups, and flavonoids, and other chemical constituents are saponins, essential oil, carbohydrate etc 5 . According to previous researches W. fruticosa flowers extract has anti-inflammatory 6 , gastroprotective 7 , hepatoprotective 8 , antioxidant 9 , Alzheimer's disease 10 , and wound healing activity 11 . The main objective of this work was to explore the phytochemical profile, antioxidant activity, and in vitro anti-diabetic properties of the W. fruticosa flower.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Antidiabetic activity of hydroalcoholic flower extract of Woodfordia fruticosa (L.) Kurz

Giri

Dey

Dua

2022

J. Drug Delivery Ther.

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Woodfordia fruticosa (L.) Kurz, (W. fruticosa) a member of the Lythraceae family, commonly known as dhaiphul, has been used in traditional medicine to cure common illnesses since ancient times. The most effective part of this plant is the flower which has a high demand in the national as well as the international market of the eastern south part of Asian countries. The present study focuses on exploring the presence of phytochemicals, antioxidants, and antidiabetic potential of hydroalcoholic extract of flower of W. fruticosa. Phytochemical test showed the presence of saponin, flavonoid, carbohydrates, alkaloids, tannin, anthraquinone, and triterpenoids in the extract, and it showed an effective result of total phenolic content, total flavonoid Content with the value of 371.96 ± 2.86 mg of gallic acid equivalent per gram of the extract and 113.82 ± 0.178 mg of quercetin equivalent per gram of dry weight. In vitro antioxidant assay, for the DPPH assay, the IC50 values were found 30.85 ± 0.36 μg/ml and 22.35 ± 0.54 μg/ml for the extract of W. fruticosa flower and ascorbic acid respectively and for the FRAP Assay, the reducing power assay of plant extract is lesser than standard (Ascorbic acid). W. fruticosa also showed significant hyperglycemic activity. In accordance with results, we can conclude that plant consists of a high polyphenolic and flavonoid group which help in antioxidant activity as well as the hydroalcoholic extract shows mild activity against diabetes. Keywords: Woodfordia fruticosa, phytochemical, hydroalchoholic, antioxidants, antidiabetic, DPPH.

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Preclinical development of gastro-protective botanical candidate from Woodfordia fruticosa Chemical standardization, efficacy, pharmacokinetics and safety pharmacology

Cited by 11 publications

References 12 publications

Antioxidant and antimicrobial extracts obtained from agricultural by-products: Strategies for a sustainable recovery and future perspectives

Antioxidant and antimicrobial extracts obtained from agricultural by-products: Strategies for a sustainable recovery and future perspectives

Preclinical Development of Mahanine-Enriched Fraction from Indian Spice Murraya koenigii for the Management of Cancer: Efficacy, Temperature/pH stability, Pharmacokinetics, Acute and Chronic Toxicity (14-180 Days) Studies

Antioxidant and Antidiabetic activity of hydroalcoholic flower extract of Woodfordia fruticosa (L.) Kurz

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