Preclinical evaluation of antihyperglycemic activity of Clerodendron infortunatum leaf against streptozotocin-induced diabetic rats

Abstract

IntroductionClerodendron infortunatum Linn. (Verbenaceae), commonly known as Bhant in Hindi, is a small shrub occurring throughout the plains of India, which is traditionally used for several medicinal purposes. The aim of the present study was to evaluate the preclinical antihyperglycemic activity of the methanol extract of the leaves of C. infortunatum (MECI) in Wistar rats.MethodsHyperglycemia was induced in rats by a single intraperitoneal injection of streptozotocin (STZ, 65 mg/kg body weight). Three days after STZ induction, the hyperglycemic rats were treated with MECI intraperitoneally at the doses of 250 and 500 mg/kg body weight daily for 15 days. Glibenclamide (0.5 mg/kg, orally) was used as a reference drug. The fasting blood glucose levels were measured on every fifth day during the 15 days of treatment. Serum biochemical parameters such as glutamate pyruvate transaminase, glutamate oxaloacetate transaminase, alkaline phosphatase, cholesterol, and total protein were estimated. Antioxidant properties were assessed by estimating hepatic lipid peroxidation, reduced glutathione (GSH), and catalase (CAT).ResultsMECI at the doses of 250 and 500 mg/kg intraperitoneally significantly (P<0.001) and dose-dependently reduced and normalized blood glucose levels as compared to that of the STZ control group. Serum biochemical parameters were significantly (P<0.001) restored towards normal levels in MECI-treated rats as compared to the STZ control. MECI treatment also significantly (P<0.001) decreased lipid peroxidation and recovered GSH levels and CAT activity towards normal values, as compared to the STZ control.ConclusionThe present study demonstrated that the leaves of C. infortunatum had remarkable preclinical antihyperglycemic activity in STZ-induced diabetic rats.

Antidiabetic Potential of Perillyl Alcohol, a Monoterpene on High-Fat Diet Low-Dose Streptozotocin-Induced Diabetes in Experimental Rats

“…From the OGTT data, it is clear that administration of POH at the dose 50 mg/kg and 100 mg/kg effectively prevented the increase in plasma glucose level without causing hypoglycemia in rats as efficiently as the reference drug glibenclamide. This result of OGTT confirms that the reduction of intestinal glucose transporter is similar to the finding [27]. Treatment with HFD and low dose of STZ (35 mg/kg)-induced diabetic rats started reducing FBG levels in a dose-dependent manner after 10 days, 20 days, and made them normoglycemic after 30 days.…”

In vitro antioxidant and in vivo antidiabetic, antihyperlipidemic activity of linseed oil against streptozotocin‐induced toxicity in albino rats

“…GSH may play an important role in protecting the tissue from peroxidative attack. STZ significantly increased the level of tissue GSH, which contradicts the findings of the previous studies 32. The present effect could possibly be attributed to the increased biogenesis of GSH due to oxidative stress as a consequence of feedback mechanism.…”

Hypoglycaemic effect ofMelothria heterophyllain streptozotocin-induced diabetic rats

“…Four days after diabetes induction, the OGTT was performed by feeding glucose in the form of a solution through orally to STZ-induced diabetic rats fasted for 18 h. The animals were divided into nine groups, each having six animals. Group-I served normal control and received normal saline only; the animals of group II were administered STZ only; group III-IV were treated with EEMH 200 and 400 mg/kg, b.w., p.o; group V-VI with GA 2 and 4 mg/kg, b.w., p.o; group VII-VIII with RU 2 and 4 mg/kg, b.w., p.o and group IX was treated with glibenclamide (a known hypoglycemic agent) 0.5 mg/kg (Das et al, 2011), respectively. One hour later, glucose (2 g/kg) was administrated.…”