Preclinical evaluation of immunogenicity, efficacy and safety of a recombinant plant-based SARS-CoV-2 RBD vaccine formulated with 3M-052-Alum adjuvant

Immunogenicity of a recombinant plant-produced respiratory syncytial virus F subunit vaccine in mice

“…3M-052 is a synthetic small molecule adjuvant that acts as a toll-like receptor (TLR) 7/8 agonist, activating specific receptors in the immune system and triggering and enhancing the immune response. These adjuvants have been selected based on their safety profile, ability to enhance the immune response to the desired level, and compatibility with the various vaccine antigens [ [41] , [42] , [43] , [44] ]. In mice, RSV-F-Fc fusion protein induced RSV-specific titer after intramuscular injection with a 2-dose prime–boost strategy.…”

Nicotiana benthamiana as a potential source for producing anti-dengue virus D54 neutralizing therapeutic antibody

“…Moreover, the transient expression with agroinfiltration is more appropriate for producing therapeutic anti-infective agents since it is more flexible for protein switching [ 44 ]. For scalability, this method is extensively used for industrial-scale production of therapeutic proteins, including the Baiya SARs-CoV-2 protein subunit vaccine, currently in phase 2 clinical trials [ 47 ]. The produced protein by this method is sufficiently supplied throughout clinical study.…”

Plant-produced RBD and cocktail-based vaccine candidates are highly effective against SARS-CoV-2, independently of its emerging variants

“…Since RBD of SARS-CoV-2 is the primary target for potent virus-neutralizing antibodies ( Barnes et al., 2020 ; Brouwer et al., 2020 ; Piccoli et al., 2020 ; Wang et al., 2020 ; Yang et al., 2020 ), it is frequently used for COVID-19 vaccine development ( Diego-Martin et al., 2020 ; Rattanapisit et al., 2020 ; Shin et al., 2021 ; Siriwattananon et al., 2021 ; Phoolcharoen et al., 2023 ) and as an antigen in serological assays and diagnostic reagents ( Amanat and Krammer, 2020 ; Rattanapisit et al., 2020 ; Makatsa et al., 2021 ; Jirarojwattana et al., 2023 ). Several research groups have utilized N. benthamiana plants to produce different amino acid regions of RBD variants of SARS-CoV-2 ( Diego-Martin et al., 2020 ; Rattanapisit et al., 2020 ; Shin et al., 2021 ; Siriwattananon et al., 2021 ).…”