Preclinical Experiments on the Release Behavior of Biodegradable Nanofibrous Multipharmaceutical Membranes in a Model of Four-Wall Intrabony Defect

Abstract

e Guided tissue regeneration (GTR) therapy has been widely used to regenerate lost periodontium from periodontal disease. However, in terms of regenerative periodontal therapy, a multidrug-loaded biodegradable carrier can be even more promising in dealing with periodontal disease. In the current study, we fabricated biodegradable nanofibrous collagen membranes that were loaded with amoxicillin, metronidazole, and lidocaine by an electrospinning technique. The in vitro release behavior and the cytotoxicity of the membranes were investigated. A four-wall intrabony defect was created in rabbits for in vivo release analysis. The bioactivity of the released antibiotics was also examined. The experimental results showed that the drug-loaded collagen membranes could provide sustainable release of effective amoxicillin, metronidazole, and lidocaine for 28, 56, and 8 days, respectively, in vivo. Furthermore, the bioactivity of the released antibiotics remained high, with average bioactivities of 50.5% for amoxicillin against Staphylococcus aureus and 58.6% for metronidazole against Escherichia coli. The biodegradable nanofibrous multipharmaceutical membranes developed in this study may provide a promising solution for regenerative periodontal therapy.

Lidocaine subgingival irrigation modulate the levels of prostaglandin E2 in gingival crevicular fluid after periodontal therapy

“…In a recent study a potent inhibition of PGE 1 and PGE 2 release was demonstrated when treating the burned skin in the intact animal with a topical local anesthetic cream (Yregård et al, 2001), thus confirming an earlier report showing reduced PGE 2 release from isolated pieces of gastric mucosa by lidocaine (Goel et al, 1994). These inhibitory effects on PGE 2 , known to play a significant role in the mechanisms responsible for inflammatory pain, could account for some of the potent analgesic effects of intravenous lidocaine reported in burn patients (Jönsson et al, 1999, Cassuto andTarnow, 2003) and in patients having undergone surgery (Cassuto et al, 1995;Chen et al, 2013).…”

“…Indeed, LD group revealed significant improvement to control PGE 2 levels compared to control group, 1 month and 3 months after periodontal treatment, suggesting that lidocaine subgingival irrigation can modulate inhibitory effect on inflammatory process. Recently, studies have correlated the high levels of PGE 2 in sites with periodontal disease and suggested that PGE 2 can mediate tissue destruction during periodontal disease (Chen et al, 2013;Sánchez et al, 2013). Kumar et al (2013) reported that chronic periodontitis subjects treated by non-surgical periodontal therapy-SRP have mean PGE 2 concentrations in GCF and statistically it reduced significantly after periodontal therapy.…”

Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration

“…To that end, several controlled-release systems, such as Atridox ® (doxycycline-based) and PerioChip ® (chlorhexidine-based), have been thoroughly used to locally deliver antimicrobial agents to the periodontal pocket, resulting in positive clinical effects [13, 14]. More recently, antimicrobial agents have been incorporated into GTR/GBR membranes, endowing to them antibacterial activity [6, 15]. Notwithstanding, it is well recognized that antibiotics may produce some important side-effects, mainly those related to bacterial strain resistance, which is a current global concern, since bacteria are becoming resistant to several antibiotic therapies [4, 6].…”

Impact of supramolecular interactions on swelling and release behavior of UPy functionalized HEMA‐based hydrogels

“…D, Comparison of the swelling ratio between PH‐S6 and PH‐C6. ** Toxicity level of the drug is as reported in the literature [Colour figure can be viewed at wileyonlinelibrary.com]…”