Preclinical induced membrane model to evaluate synthetic implants for healing critical bone defects without autograft

DeBaun, Malcolm R.; Stahl, Alexander M.; Daoud, Adam I.; Chen, Pan; Bishop, Julius A.; Gardner, Michael J.; Yang, Yunzhi Peter

doi:10.1002/jor.24153

Cited by 25 publications

(41 citation statements)

References 53 publications

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“…The time point for pharmaceutical interventions as well as the concentrations of BMP-7 and bisphosphonate used were determinants in the success of the healing. Of note, insertion of a 3D printed polycaprolactone (PLC)/β-tricalcium phosphate (β-TCP) scaffold in a rat IM model has been shown to support bone formation (DeBaun et al, 2018). It was not the case when a natural coral scaffold (99% calcium carbonate and 1% organic material) was used to fill in the IM in a sheep model (Viateau et al, 2007).…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

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The Masquelet technique: Current concepts, animal models, and perspectives

Klein

Monet

Barbier

et al. 2020

J Tissue Eng Regen Med

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Bone reconstruction within a critical‐sized defect remains a real challenge in orthopedic surgery. The Masquelet technique is an innovative, two‐step therapeutic approach for bone reconstruction in which the placement of a poly (methylmethacrylate) spacer into the bone defect induces the neo‐formation of a tissue called “induced membrane.” This surgical technique has many advantages and is often preferred to a vascularized bone flap or Ilizarov's technique. Although the Masquelet technique has achieved high clinical success rates since its development by Alain‐Charles Masquelet in the early 2000s, very little is known about how the process works, and few animal models of membrane induction have been developed. Our successful use of this technique in the clinic and our interest in the mechanisms of tissue regeneration (notably bone regeneration) prompted us to develop a surgical model of the Masquelet technique in rats. Here, we provide a comprehensive review of the literature on animal models of membrane induction, encompassing the defect site, the surgical procedure, and the histologic and osteogenic properties of the induced membrane. We also discuss the advantages and disadvantages of those models to facilitate efforts in characterizing the complex biological mechanisms that underlie membrane induction.

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Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

“…The replacement of the IM by a PLC-based film did not improve bone regeneration in rat. (DeBaun et al, 2018).…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

The Masquelet technique: Current concepts, animal models, and perspectives

Klein

Monet

Barbier

et al. 2020

J Tissue Eng Regen Med

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“…Treatment of bone defects of a critical size remains a serious challenge in surgery and orthopedics. Segment defects are the result of various injuries, infections, resections of tumors of various localization, as well as congenital deformity of bone development (1,2,4). Conceivably, bone defects of a critical size do not heal spontaneously, despite the surgical stabilization (3,4).…”

Section: Introductionmentioning

confidence: 99%

“…Segment defects are the result of various injuries, infections, resections of tumors of various localization, as well as congenital deformity of bone development (1,2,4). Conceivably, bone defects of a critical size do not heal spontaneously, despite the surgical stabilization (3,4). The method of using autologous bone grafts in reconstructive surgery for congenital malformations and defects caused by some trauma or disease, as well as for cosmetic procedures in certain parts of the body, have their disadvantages, such as a limited bone scraping, non-identity of bone shape, discomfort and pain in the area of the donor site.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional TCP scaffolds enriched with Erythropoietin for stimulation of vascularization and bone formation

et al. 2019

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In our work, we conducted studies to evaluate the reconstruction of an artificially created critical radial defect in rats with the use of tri-calcium phosphate scaffold enriched with erythropoietin (ЕРО). A model of the bone defect on the radius forearm in rats of a critical size has been developed. This model allows to conduct the study without the use of osteosynthesis. EPO is a well-known hormone which regulates formation of the red blood cells (2, 3). ЕРО increases the expression of VEGF and promotes angiogenesis (3, 4). Therefore, EPO may have a great potential for use as a growth factor for angiogenesis and play its particular role in the bone tissue regeneration. There are no published studies that describe interactions between EPO and biomaterials for development of the bone tissue. Thus, the purpose of this study was to evaluate the interaction between EPO and tri-calcium phosphate scaffold (TCP) with a well-studied biocompatibility and the given porosity, as well as to determine whether EPO in the enriched TCP will promote the bone regeneration. The X-ray analysis was performed in 10 days, 28 days and 3 months after the surgery. The histological analysis was performed in 28 days and 3 months after the surgery. The results have demonstrated that the complex of TCP scaffold with erythropoietin is a promising growth factor for stimulating the development of the bone tissue, since the TCP scaffold enriched with erythropoietin is very easy to obtain in a noninvasive and simple procedure, as well as the complex promotes interaction with the surrounding tissues and induces the bone regeneration.

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“…We used the filaments and subcutaneous implantation as a simple screening test before we test them in bone defects. In our other studies, we implanted 3D-printed TCP/PCL scaffolds of single formulation (20% TCP/80% PCL) into large bone defects in rabbit femoral heads [13,14] and rat femurs [46]. Both implantations showed that our scaffolds promoted bone ingrowth into the porous structure and retained structural integrity, suggesting excellent biocompatibility and osteointegration.…”

Section: Histological Examinationmentioning

confidence: 84%

The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue Engineering

et al. 2020

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We evaluated the effect of electron beam (E-beam) sterilization (25 kGy, ISO 11137) on the degradation of β-tricalcium phosphate/polycaprolactone (β-TCP/PCL) composite filaments of various ratios (0:100, 20:80, 40:60, and 60:40 TCP:PCL by mass) in a rat subcutaneous model for 24 weeks. Volumes of the samples before implantation and after explantation were measured using micro-computed tomography (micro-CT). The filament volume changes before sacrifice were also measured using a live micro-CT. In our micro-CT analyses, there was no significant difference in volume change between the E-beam treated groups and non-E-beam treated groups of the same β-TCP to PCL ratios, except for the 0% β-TCP group. However, the average volume reduction differences between the E-beam and non-E-beam groups in the same-ratio samples were 0.76% (0% TCP), 3.30% (20% TCP), 4.65% (40% TCP), and 3.67% (60% TCP). The E-beam samples generally had more volume reduction in all experimental groups. Therefore, E-beam treatment may accelerate degradation. In our live micro-CT analyses, most volume reduction arose in the first four weeks after implantation and slowed between 4 and 20 weeks in all groups. E-beam groups showed greater volume reduction at every time point, which is consistent with the results by micro-CT analysis. Histology results suggest the biocompatibility of TCP/PCL composite filaments.

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Preclinical induced membrane model to evaluate synthetic implants for healing critical bone defects without autograft

Cited by 25 publications

References 53 publications

The Masquelet technique: Current concepts, animal models, and perspectives

The Masquelet technique: Current concepts, animal models, and perspectives

Three-dimensional TCP scaffolds enriched with Erythropoietin for stimulation of vascularization and bone formation

The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue Engineering

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